DACASTO MAURO

Contacts
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Structure Department of Comparative Biomedicine and Food Science
Telephone 0498272935
Qualification Professore ordinario
Scientific sector VET/07 - VETERINARY PHARMACOLOGY AND TOXICOLOGY
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Office hours
People can meet Prof. Dacasto at his home office (Agripolis Legnaro, Dept. of Comparative Biomedicine and Food Science, "Museo" building, first floor) It would be far better to make an appointment with Prof. Dacasto (tel. +39.049.827.2935; email: mauro.dacasto@unipd.it)
É possibile incontrare il Prof. Dacasto presso il proprio ufficio (Agripolis Legnaro, Dipartimento di Biomedicina comparata e Alimentazione, Edificio "Museo", primo piano) É preferibile prendere appuntamento telefonando direttamente al Prof. Dacasto (tel. +39.049.827.2935) o inviando una email (mauro.dacasto@unipd.it)
(updated on 13/08/2018 10:45)

Proposals for thesis
PROPOSED DEGREE THESES OR INTERNSHIPS

Topic
Subjects or projects for degree theses or internships are linked to Proff. Dacasto and Giantin’s research topics.
In particular:
• Comparative in vitro and in vivo studies on the expression and regulation of drug metabolizing enzymes and afflux/efflux drug transporters
• Xenobiotic molecular and cellular toxicity
• Veterinary oncology and anticancer chemotherapy
• Veterinary Pharmacogenetics and Toxicogenomics

Length
Variable, from 1-3 months and up to 2 years (depending from the subject, the Bachelor/Master of Science to whom the student belongs and, consequently, his/her frequency of laboratories)

Vacancy
Variable (depending on the available subjects/projects)

Requirements
• availability to attend either the Pharmacogenetic/Toxicogenomic than the Cellular Biology Laboratories of the Division of Veterinary Pharmacology & Toxicology research unit
• interest for basic and applied pharmaco-toxicological sciences

Methodologies the student might learn
Molecular (including NGS) and cellular biology techniques

For further information, send an email to Prof. Dacasto (mauro.dacasto@unipd.it) or contact him by phone (+39.049.827.2935)

Curriculum Vitae


Lecturer's Curriculum (PDF): D0636A0EC09B30872F59A9DCE0BCD96E.pdf

Research areas
Research Topics

a. Comparative in vitro and in vivo studies on the expression and regulation of drug metabolizing enzymes (P450-dependent monooxygenases, conjugative enzymes, drug transporters).
In particular:
a.1. importance of constitutive factors, e.g. sex, age, species, breed/strain, individual susceptibility (pharmacogenetics);
a.2. identification of P450 isoforms responsible for the oxidative metabolism of different chemotherapeutics and contaminants;
a.3. effects of the exposure to xenobiotics (including also natural compounds, diet constituents and illicit growth promoters), on the expression and regulation of drug metabolizing enzymes and drug transporters (i.e. induction/inhibition phenomena).

b. Xenobiotic molecular and cellular toxicity.
This topic includes:
b.1. xenobiotic cytotoxicity testing;
b.2. in vitro studies on biochemical and molecular mechanisms of action of xenobiotics (i.e. apoptosis/necrosis) on target or surrogate tissues (i.e. liver, immune system).

c. Veterinary oncology and anti-cancer chemotherapy
c.1. Next Generations Sequencing approach to companion animal neoplasms
c.2. patient geno- and phenotyping (pharmacogenetics);
c.3. in vitro/in vivo studies on new therapeutic targets and characterization of their bio-molecular effects

d. Veterinary Pharmacogenetics and Toxicogenomics
In particular:
d.1. studies concerning the effects of xenobiotics (including natural compounds, diet constituents) on the transcriptome of veterinary species;
d.2. studies devoted to the definition of biochemical/molecular biomarkers of internal dose, effect or susceptibility (e.g. genetic polymorphisms) to be used for screening purposes, in biomonitoring as well as in the clinics.

Methods and techniques

1. Biochemical techniques (subcellular fractions isolation, enzymatic activities, immunoblotting)
2. Cell biology techniques (cultures and transfection of established cell lines, primary cell cultures, cytotoxicity testing, enzymatic activity in culture)
3. Molecular biology techniques (DNA/RNA isolation, PCR end point, Real time PCR, cloning, sequencing, cDNA microarray, Next Generation Sequencing approaches, e.g., RNA-seq).

Research Unit Staff

Principal Investigator
Mauro DACASTO (Full Prof., DVM, PhD, Dipl. ECVPT)
Members
Francesca CAPOLONGO (Associate Prof., MSc, PhD, limited to topic a)
Mery GIANTIN (Associate Prof.,. DVM, PhD)
Rosa Maria LOPPARELLI (Technician, MSc, PhD)
Lorena LUCATELLO (Technician, MSc, PhD, limited to research topic a)
Roberta MERLANTI (Assistant Prof., DVM, PhD, limited to research topic a)
Marianna PAULETTO (Assistant Prof., MSc, PhD)
Eleonora ZORZAN (MSc, PhD, post-doc)

Publications


Lecturer's Publications (PDF): D0636A0EC09B30872F59A9DCE0BCD96E.pdf

List of taught course units in A.Y. 2018/19
Degree course code (?) Degree course track Course unit code Course unit name Credits Year Period Lang. Teacher in charge
MV0991 COMMON AVP7080706 7 4th Year Second
semester
ITA FLAVIANA GOTTARDO
IF0362 002LE AVP5073779 8 2nd Year First
semester
ENG MERY GIANTIN
AV2378 COMMON AVP7077493 8 2nd Year Second
semester
ENG MAURO DACASTO
IF0362 001LE AVP6075240 12 2nd Year First
semester
ITA MERY GIANTIN
MV0991 COMMON AVP7079917 9 3rd Year Annual ITA MAURO DACASTO