PROVVEDI ROBERTA

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Telephone 0498272346
Qualification Professore associato confermato
Scientific sector BIO/19 - GENERAL MICROBIOLOGY
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Proposals for thesis
Role of SigE in the stringent response of Mycobacterium smegmatis and Mycobacterium tuberculosis

Curriculum Vitae
ROBERTA PROVVEDI (RP), was born in Siena on November 29th 1967. Resident in Padova, (Italy)
Present position: Associate Professor of Microbiology (SSD BIO/19 - General Microbiology) at the Department of Biology, University of Padova.
She is a member of the Italian Society of General Microbiology and Microbial Biotechnology (SIMGBM).

Education

-1992. Bachelor in Biology
-1999. PhD in Microbiology

Professional activity

After her Bachelor Degree, RP has always been involved in studies on Microbiology. She worked at the Institute of Microbiology, Department of Molecular Biology, University of Siena (1992-1995). From 1995 to 2000 she worked first as a PhD STUDENT and then as a RESEARCH ASSOCIATE at the Public Health Research Institute of New York, NY, USA. In 2000 she was back in Siena and in 2002 she moved to the Department of Histology, Microbiology and Medical Biotechnology of the University of Padova. In 2006 she had a position as RESEARCH ASSOCIATE and from 2015 she is ASSOCIATE PROFESSOR in Microbiology (SSD BIO/19 – General Microbiology) at the Department of Biology, University of Padova

Teaching experience

Courses teached by RP:

2009/2010. APPLIED MICROBIOLOGY, degree in Health Biology, University of Padova
2011/present. MICROBIOLOGY, degree in Biology, University of Padova
2011/present. MICROBIOLOGY AND DIAGNOSTIC LABORATORY, degree in Health Biology, University of Padova


Research areas
RP has principally worked in the fields of bacterial genetics and global regulation of gene expression. At the beginning of her career, RP studied conjugative transposons of Gram positive bacteria and developed biotechnological tools with the commensal Streptococcus gordonii. From 1995 to 2000 she was in New York (USA). During this period her research focused on the characterization of the molecular mechanisms involved in natural transformation in the Gram positive bacterium Bacillus subtilis. When she moved to Padova, she started a research focused on Mycobacterium tuberculosis (Mtb) with main interest on the relationship between virulence and global regulation of transcription.

Publications
Donà V, Rodrigue S, Dainese E, Palù G, Gaudreau L, Manganelli R, Provvedi R. (2008)
Evidence of complex transcriptional, translational, and posttranslational regulation of the
extracytoplasmic function sigma factor sigmaE in Mycobacterium tuberculosis. J Bacteriol.
190(17):5963-71.
Provvedi R, Kocíncová D, Donà V, Euphrasie D, Daffé M, Etienne G, Manganelli R, Reyrat JM.
(2008) SigF controls carotenoid pigment production and affects transformation efficiency and
hydrogen peroxide sensitivity in Mycobacterium smegmatis. J Bacteriol. 190(23):7859-63.
Milano A, Pasca MR, Provvedi R, Lucarelli AP, Manina G, Ribeiro AL, Manganelli R, Riccardi
G. (2009) Azole resistance in Mycobacterium tuberculosis is mediated by the MmpS5-MmpL5 efflux
system. Tuberculosis 89(1):84-90.
Provvedi R, Boldrin F, Falciani F, Palù G, Manganelli R. (2009) Global transcriptional response to
vancomycin in Mycobacterium tuberculosis. Microbiology. 155(Pt 4):1093-102.
Manganelli R, Provvedi R. (2010) An integrated regulatory network including two positive feedback
loops to modulate the activity of sigma(E) in mycobacteria. Mol Microbiol. 75(3):538-42.
Cascioferro A, Boldrin F, Serafini A, Provvedi R, Palù G, Manganelli R. (2010) Xer site-specific
recombination, an efficient tool to introduce unmarked deletions into mycobacteria. Appl Environ
Microbiol. 76(15):5312-6.
Casonato S, Provvedi R, Dainese E, Palù G, Manganelli R. (2014) Mycobacterium tuberculosis
requires the ECF sigma factor SigE to arrest phagosome maturation. PLoS One, 9(9):e108893. Erratum
in: PLoS One. 2014;9(12):e115990.
Delogu G, Provvedi R, Sali M, Manganelli R. (2015) Mycobacterium tuberculosis virulence:
insights and impact on vaccine development. Future Microbiol. 10(7):1177-94. Review.
Degiacomi G, Benjak A, Madacki J, Boldrin F, Provvedi R, Palù G, Kordulakova J, Cole ST,
Manganelli R. (2017) Essentiality of mmpL3 and impact of its silencing on Mycobacterium
tuberculosis gene expression. Sci Rep. 7:43495.
Rosado LA, Wahni K, Degiacomi G, Pedre B, Young D, de la Rubia AG, Boldrin F, Martens E,
Marcos-Pascual L, Sancho-Vaello E, Albesa-Jové D, Provvedi R, Martin C, Makarov V, Versées
W, Verniest G, Guerin ME, Mateos LM, Manganelli R, Messens J. (2017) The antibacterial
prodrug activator Rv2466c is a mycothiol-dependent reductase in the oxidative stress response of
Mycobacterium tuberculosis. J Biol Chem. 292(32):13097-13110.
Perrone R, Lavezzo E, Riello E, Manganelli R, Palù G, Toppo S, Provvedi R, Richter SN. (2017)
Mapping and characterization of G-quadruplexes in Mycobacterium tuberculosis gene promoter
regions. Sci Rep. 7(1):5743.
Montoya-Rosales A, Provvedi R, Torres-Juarez F, Enciso-Moreno JA, Hernandez-Pando R,
Manganelli R, Rivas-Santiago B. (2017) lysX gene is differentially expressed among Mycobacterium
tuberculosis strains with different levels of virulence. Tuberculosis (Edinb). 106:106-117.
Pisu D, Provvedi R, Espinosa DM, Payan JB, Boldrin F, Palù G, Hernandez-Pando R,
Manganelli R. (2017) The Alternative Sigma Factors SigE and SigB Are Involved in Tolerance and
Persistence to Antitubercular Drugs. Antimicrob Agents Chemother. 61(12). pii: e01596-17.
Boldrin F, Degiacomi G, Serafini A, Kolly GS, Ventura M, Sala C, Provvedi R, Palù G, Cole ST,
Manganelli R. (2017) Promoter mutagenesis for fine-tuning expression of essential genes in
Mycobacterium tuberculosis. Microb Biotechnol. 11(1):238-247.

List of taught course units in A.Y. 2018/19
Degree course code (?) Degree course track Course unit code Course unit name Credits Year Period Lang. Teacher in charge
SC1177 COMMON SCN1032639 8 1st Year First
semester
ITA ROBERTA PROVVEDI
SC1165 COMMON SCO2043653 6 2nd Year Second
semester
ITA ROBERTA PROVVEDI