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Structure Department of Biology
Telephone 0498276456
Qualification Professore associato confermato
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Office hours
Tuesday from 10:30 to 12:30 Dipartimento di Biologia 5° piano sud
(updated on 25/06/2018 13:38)

Proposals for thesis
Relationships between telomere metabolism and dNTP pool sizes. The aim of the project is to examine the effect of dNTP imbalance on telomere length by quantitative fluorescence in situ hybridization (Q-FISH) and quantitative PCR.

Deoxynucleotide imbalance and mtDNA stability in mammalian cells. The aim of the project is to understand cellular mechanisms for the maintenance of mtDNA integrity and copy number by Next Generation Sequencing and quantitative PCR.

Curriculum Vitae
Affiliation: Dept. of Biology

Position: Associate Professor at the Department of Biology of the University of Padova, Italy

Education and academic career:
1994: Laurea in Biological Sciences, University of Padova
1995: Erasmus Fellowship, Department of Genetics, University of Stockholm, Sweden
1996-1998: PhD student at the Department of Biology, University of Padova
1997: EMBO short-term Fellowship, Department of Biochemistry, Karolinska Institute, Stockholm, Sweden
1998: EMBO short-term Fellowship, Department of Biochemistry, Karolinska Institute, Stockholm, Sweden
1999-2003: Postdoctoral fellow, Department of Biology, University of Padova
2004-2017: Assistant Professor in Cell Biology, Department of Biology, University of Padova
2017-present: Associate Professor in Cell Biology, Department of Biology, University of Padova

Co-author of 30 peer-reviewed papers
Total citation: 956
H factor: 18

Research areas
Regulation of DNA precursors in mammalian cells during proliferation and quiescence: implications for the stability of the nuclear and mitochondrial genomes in health and disease.
Disturbances of the relative concentrations and the dynamics of DNA precursor (dNTP) pools affect the accuracy and efficiency of DNA synthesis. Main effects are increased frequency of mutations, structural alterations of chromosomes, destabilization or depletion of the mitochondrial genome. We study the mechanisms that keep dNTP pools in tune with the needs of nuclear and mitochondrial DNA replication and repair. The enzyme network includes both synthetic and catabolic enzymes and we analyze their functional interactions in cultured mammalian cells.

Publications (last 10 years)

G Santinon, I Brian I, A Pocaterra, P Romani, E Franzolin, C Rampazzo, A Bicciato, and S. Dupont (2018) “dNTP metabolism links YAP/TAZ and mechanical cues to cell growth and oncogene-induced senescence” EMBO Journal. e97780.

D Pajalunga, E Franzolin, M Stevanoni, S Zribi, N Passaro, A Gurtner, S Donsante, D Loffredo, L Losanno, V Bianchi, A Russo, C Rampazzo and M Crescenzi (2017) “A defective dNTP pool hinders DNA replication in cell cycle-reactivated terminally differentiated muscle cells.” Cell Death and Differentiation, 24(5):774-784.

C Rampazzo, MG Tozzi, C Dumontet, L P Jordheim (2016) “The druggability of intracellular nucleotide degrading enzymes”. Cancer Chemotherapy and Pharmacology 77: 883-893.

E Franzolin, C Salata, V Bianchi, C Rampazzo (2015) “The Deoxynucleoside Triphosphate Triphosphohydrolase Activity of SAMHD1 Protein Contributes to the Mitochondrial DNA Depletion Associated with Genetic Deficiency of Deoxyguanosine Kinase”. Journal of Biological Chemistry 290:25986-96.

C Miazzi, P Ferraro, G Pontarin, C Rampazzo, P Reichard, and V Bianchi (2014) “Allosteric regulation of the human and mouse deoxyribonucleotide triphosphohydrolase sterile α-motif /histidine-aspartate domain containing protein 1 (SAMHD1)” Journal of Biological Chemistry 289: 18339-18346.

E. Franzolin, G. Pontarin, C. Rampazzo, C. Miazzi, P Ferraro, E. Palumbo, P. Reichard and V. Bianchi (2013) “The deoxynucleotide triphosphohydrolase SAMHD1 is a major regulator of DNA precursor pools in mammalian cells” Proceedings of the National Academy of Sciences of the United States of America 110:14272-7.

M. Frangini, E. Franzolin, F. Chemello, P. Laveder, C. Romualdi, V. Bianchi and C. Rampazzo (2013) “Synthesis of mitochondrial DNA precursors during myogenesis, an analysis in purified C2C12 myotubes” Journal of Biological Chemistry 288: 5624-5635.

E. Franzolin, C. Miazzi, M. Frangini, E. Palumbo, C. Rampazzo, V. Bianchi (2012) “The pyrimidine nucleotide carrier PNC1 and mitochondrial trafficking of thymidine phosphates in cultured human cells”. Experimental Cell Research 318: 2226-2236.

G. Pontarin, P. Ferraro, C. Rampazzo, G. Kollberg, E. Holme, P.Reichard, V. Bianchi (2011) “Deoxyribonucleotide metabolism in cycling and resting human fibroblasts with a missense mutation in p53R2, a subunit of ribonucleotide reductase”. Journal of Biological Chemistry 286:11132-40.

C. Rampazzo, C. Miazzi, E. Franzolin, G. Pontarin, P. Ferraro, M. Frangini, P. Reichard, V Bianchi. (2010) “Regulation by degradation, a cellular defence against deoxyribonucleotide pool imbalances”. Mutation Research 703: 2-10.

M. Frangini, C. Rampazzo, E. Franzolin, MC. Lara, MR. Vilà, R. Martí, V. Bianchi. (2009) “Unchanged thymidine triphosphate pools and thymidine metabolism in two lines of thymidine kinase 2-mutated fibroblasts”. FEBS Journal. 276: 1104-1113.

G. Pontarin, A. Fijolek, P. Pizzo, P. Ferraro, C. Rampazzo, T. Pozzan, L. Thelander, P. Reichard, V. Bianchi (2008) “ Ribonucleotide reduction is a cytosolic process in mammalian cells independently of DNA damage” Proceedings of the National Academy of Sciences of the United States of America 105: 17801-17806.

C. Rampazzo, S. Fabris, E. Franzolin, K. Crovatto, M Frangini, and V. Bianchi “ Mitochondrial thymidine kinase and the enzymatic network regulating thymidine triphosphate pools in cultured human cells” (2007) Journal of Biological Chemistry 282: 3

List of taught course units in A.Y. 2018/19
Degree course code (?) Degree course track Course unit code Course unit name Credits Year Period Lang. Teacher in charge
SC2445 COMMON SCP8085218 9 1st Year First
SC1731 COMMON SCN1037574 8 1st Year Second
SC1731 COMMON SCN1037601 6 1st Year First