BARCACCIA GIANNI

Contacts
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Structure Department of Agronomy, Food, Natural Resources, Animals and the Environment
Telephone 0498272814
Qualification Professore ordinario
Scientific sector AGR/07 - AGRARIAN GENETICS
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Notice and additional information
Responsabile di BreedOmics – Servizi di analisi genomiche per il miglioramento genetico di specie agro-alimentari.


Tipologie dei servizi:

Estrazione e purificazione di DNA genomico totale ad alto peso molecolare
(isolamento di DNA genomico mediante mini-prep)

Isolamento di DNA genomico con sistema automatizzato
(isolamento di DNA genomico in piastre da 96 campioni)

Valutazione della qualità e della quantità di DNA genomico
(mediante analisi elettroforetica e spettrofotometrica o fluorimetrica)

Fingerprinting di DNA genomico mediante marcatori PCR-derivati di tipo STS
(analisi di marcatori SCAR in gel di agarosio con marcatura luminescente)

Fingerprinting di DNA genomico mediante marcatori PCR-RFLP
(analisi di marcatori CAPS in gel di agarosio con marcatura luminescente)

Fingerprinting di DNA genomico mediante marcatori AP-PCR e I-SSR
(analisi di marcatori multi-locus PCR-derivati con primer universali ed elettroforesi capillare)

Fingerprinting di DNA genomico mediante marcatori AFLP
(analisi di marcatori AFLP, S-SAP e M-AFLP con primer EcoRI/PstI e MseI/TaqI ed elettroforesi capillare)

Genotyping di DNA genomico mediante marcatori singolo-locus SSR
(analisi di genotipizzazione mediante SSR saggiati in multiplex attraverso elettroforesi capillare)

Barcoding di DNA mitocondriale e plastidiale mediante sequenziamento
(analisi di marcatori SNP di geni Cox1 e CytB e rbcL e trnH-psbA in matrici agro-alimentari)

Haplotyping di DNA mediante sequenziamento genico
(analisi di marcatori SNP di geni nucleari singola-copia per la definizione di aplotipi)

Elaborazione statistica dei dati sperimentali
(calcolo indici di diversità genetica e coefficienti di similarità genetica, gradi di omozigosi/eterozigosi,
determinazione di matrici di distanze genetiche, costruzione di alberi NJ e UPGMA, definizione di aplotipi e genotipi)


Caratterizzazione genetica di varietà (linee pure, cloni e ibridi F1)
(analisi dei parametri DUS e ACS in relazione al grado di omozigosi/eterozigosi e alla differenziazione
allelica tra genotipi utilizzando marcatori SSR mappati: 1 per braccio cromosomico del genoma X della specie)

Office hours
Ufficio del docente c/o DAFNAE Si riceve tutti i giorni previo appuntamento concordato telefonicamente o mediante posta elettronica
(updated on 15/07/2013 16:16)

Proposals for thesis
1) Genome analysis by means of PCR-derived molecular markers aimed at the construction of genetic linkage maps, identification of genes and marker-assisted selection (MAS);

2) Transcriptome analysis by means of mRNA profiling and NGS technologies, gene expression studies, and structural, bioinformatic and functional analysis of genes;

3) Analysis of genetic diversity and marker-assisted breeding (MAB): characterization of varieties and natural populations belonging to crops, trees and livestock species;

4) Development of molecular diagnostic assays for the genetic traceability of crop plants and fish derivatives using DNA fingerprinting and DNA barcoding.

Curriculum Vitae
Prof. Gianni Barcaccia, PhD: Curriculum vitae

Current position
Full Professor of Plant Genetics and Genomics, School of Agriculture Sciences and Veterinary Medicine, University of Padova, Italy (www.unipd.it) and Adjunct Professor of Plant Breeding, University of Georgia, Athens, USA (www.uga.edu)
Head of the Laboratory of Genomics for Plant Breeding (www.giannibarcaccia.com)
Director of the Department of Agronomy, Food, Natural resources, Animals and Environment – DAFNAE
University of Padova, Italy (www.dafnae.unipd.it)

Education
1991 – M.Sc. in Agricultural Science with Magna cum Laude, Plant Breeding Institute, Faculty of Agriculture, University of Perugia, Italy
1992 – 1995 Ph.D. in Crop Science, Genetics of Plant Reproductive Systems, Faculty of Agriculture, University of Padova, Italy
1996 – 1997 Post Doctoral Fellow in Plant Population Genetics, University of Perugia, Italy

Teaching Activities
2001 – present: Professor of Plant Genetics, also in charge of Applied Genomics, Genetic Traceability of Food Products and Plant Breeding, School of Agriculture Science and Veterinary Medicine, University of Padova (www.agrariamedicinaveterinaria.unipd.it/en)

Scientific Publications
Author of more than 100 peer-reviewed articles (101 and 106 records are available in both the Web of Science and Scopus databases, respectively, and 56 records in the NCBI PubMed database) of which 70% as first or last author or corresponding author (www.researchgate.net/profile/Gianni_Barcaccia/publications).
Books and Book Chapters: 3 academic books (1st and 2nd editions) and 8 book chapters.
Scientific publications in Italian journals: 25 articles on national journals and 2 monographs.

Research Impact Metrics
Main scientific production statistics: about 2.000 citations with H-index=26 in Scopus (35 in Google Scholar and 37 in Research Gate), total IF of publications: 225

Selected Patents
Discovery and analysis of nuclear male sterility in leaf chicory (Cichorium intybus L). International Patent Application PCT/EP2011/058765 and European Patent EP2713705-B1 (https://encrypted.google.com/patents/EP2713705B1)

Last Publications
Several original research articles and reviews were recently published in a Research Topic of Frontiers of Plant Science (www.frontiersin.org/research-topics/8183/genetics-and-genomics-of-plant-reproduction-for-crop-breeding), including Galla et al. (2019) Ovule gene expression analysis in sexual and aposporous apomictic Hypericum perforatum L. (Hypericaceae) accessions (doi: 10.3389/fpls.2019.00654), Alagna et al. (2019) The paradox of self-fertile varieties in the context of self-incompatible genotypes in olive (doi: 10.3389/fpls.2019.00725), Palumbo et al. (2019) Genomics of flower organ identity in grapevine (Vitis vinifera L.) (doi: org/10.3389/fpls.2019.00316), Palumbo et al. (2019) Construction of the first SNP-based linkage map using genotyping-by-sequencing and mapping of the male-sterility gene in leaf chicory. (doi.org/10.3389/fpls.2019.00276).

Padova, 31 October 2019

Research areas
Main Research Topics
1) Plant reproductive systems, mainly genetic control of fertilization barriers (male-sterility and self-incompatibility) and seed formation (i.e. apomixis) in crop and model species
2) Genome analysis by means of PCR-derived molecular markers aimed at the construction of genetic linkage maps, identification of genes and marker-assisted selection (MAS)
3) Transcriptome analysis by means of mRNA profiling and NGS technologies, gene expression studies, and structural, bioinformatic and functional analysis of genes
4) Analysis of genetic diversity and marker-assisted breeding (MAB): characterization of varieties and natural populations belonging to crops, trees and livestock species
5) Development of molecular diagnostic assays for the genetic traceability of crop plants and fish derivatives using DNA fingerprinting and DNA barcoding

Research Funding and Main Projects
In the last 10 years, scientific collaborations have been developed and maintained with several Italian and International groups. Among the research programs coordinated, it is worth mentioning several Projects of Relevant National Interest funded by the Ministry of University and Research, the Ministry of Agriculture Food and Forest Policies and the so-called Strategic Projects of Athenaeum funded by the University of Padova. Furthermore, research contracts have been regularly stipulated with Italian Seed Companies, mainly dealing with horticulture crops, and with Veneto Agriculture, the regional agency dealing with agriculture, forestry and food.

Publications
Scientific Publications

Author of more than 100 peer-reviewed articles (101 and 106 records are available in both the Web of Science and Scopus databases, respectively, and 56 records in the NCBI PubMed database) of which 70% as first or last author or corresponding author (www.researchgate.net/profile/Gianni_Barcaccia/publications).
Books and Book Chapters: 3 academic books (1st and 2nd editions) and 8 book chapters.
Scientific publications in Italian journals: 25 articles on national journals and 2 monographs.

Several original research articles and reviews were recently published in a Research Topic of Frontiers of Plant Science (www.frontiersin.org/research-topics/8183/genetics-and-genomics-of-plant-reproduction-for-crop-breeding), including Galla et al. (2019) Ovule gene expression analysis in sexual and aposporous apomictic Hypericum perforatum L. (Hypericaceae) accessions (doi: 10.3389/fpls.2019.00654), Alagna et al. (2019) The paradox of self-fertile varieties in the context of self-incompatible genotypes in olive (doi: 10.3389/fpls.2019.00725), Palumbo et al. (2019) Genomics of flower organ identity in grapevine (Vitis vinifera L.) (doi: org/10.3389/fpls.2019.00316), Palumbo et al. (2019) Construction of the first SNP-based linkage map using genotyping-by-sequencing and mapping of the male-sterility gene in leaf chicory. (doi.org/10.3389/fpls.2019.00276).

All scientific publications can be found and downloaded visiting the personal webpage (www.giannibarcaccia.com/publications/).

List of taught course units in A.Y. 2019/20
Degree course code (?) Degree course track Course unit code Course unit name Credits Year Period Lang. Teacher in charge
IF0362 002LE AVP5073497 8 1st Year (2019/20) First
semester
ENG GIANNI BARCACCIA
IF0362 001LE MVN1031739 10 1st Year (2019/20) First
semester
ITA GIANNI BARCACCIA
AV2293 COMMON AVP5073598 8 1st Year (2019/20) Second
semester
ENG GIANNI BARCACCIA
AG0056 COMMON AGL1000104 8 2nd Year (2019/20) First
semester
ITA GIANNI BARCACCIA