PONTARIN GIOVANNA

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Structure Department of Biology
Telephone 0498276283
Qualification Ricercatore a tempo determinato di tipo B
Scientific sector BIO/06 - COMPARATIVE ANATOMY AND CITOLOGY
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Proposals for thesis
Tesi triennale: Tesi compilativa con stesura e discussione di un lavoro scientifico pubblicato.
Tesi magistrale: il candidato contribuirà allo sviluppo di un'ipotesi di ricerca scientifica sia con un’attiva esperienza in laboratorio con metodologie attinenti alla tematica, sia elaborando e discutendo i dati in maniera critica nell'elaborato finale.
Gli argomenti riguardano lo studio del ruolo delle glutaminasi Gls1 e Gls2 nelle cellule endoteliali. Nelle parte sperimentale verranno utilizzate tecniche di biologia cellulare, biochimica, biologia molecolare.

Curriculum Vitae
Current position: Researcher RTD A, Department of Biology, University of Padova

Education: 2001 Laurea in Biological Sciences, University of Padova
2006 diploma in Clinical Pathology (scuola specializzazione), University of Padova
Professional experience:
2001-04 Fellowship, Department of Biology, University of Padova
2005-09 Research fellowship, Department of Biology, University of Padova
2009-11 Fellowship, Department of Biology, University of Padova
2011-13 Research fellowship, Department of Biology, University of Padova

Teaching: “Cell Biology” and “Techniques in mammalian culture cells” for the undergraduate students of Biotechnology master degree.

International research experience:
2005 - visiting scientist in the laboratory of Prof. Lars Thelander at the Department of Medical Biochemistry and Biophysics, Umea University, Sweden.
2014 - visiting scietist in the laboratory of Prof. Bruce Stillman at Cold Spring Harbor Laboratory, New York, U.S.A. with short-term EMBO fellowship.

Research activities: My specific interests have concerned the metabolic pathways regulating the intracellular concentration of DNA precursors (dNTPs). In particular I focused on mitochondrial dNTP (mt dNTP) pools. I developed a new method to purify mt dNTPs from mammalian cells and I investigated on mitochondrial diseases due to impaired dNTP metabolism. I set up a cellular model and studied the human mitochondrial syndrome MNGIE (Mitochodrial NeuroGastroIntestinal Encephalomyopathy) due to thymidine phosphorylase deficiency. I investigated the role of the novel ribonucleotide reductase subunit p53R2 related to mtDNA maintenance and nuclear DNA repair. I studied the physiological function of SAMHD1 as the major catabolic enzyme degrading the dNTPs related to the cell cycle progression.
At the moment I am interested in endothelial cell metabolism and in particular in the glutaminolysis pathway which is largely unexplored in vascular system. Taking advantage of my previous knowledge I started a new line of research to study the role of the glutaminases GLS1 and GLS2 related to cell proliferation and redox homeostasis using actively growing HUVECs as a model of endothelial cells during angiogenesis in normal and pathological conditions.

Research areas
Vascular metabolism in health and disease.

List of taught course units in A.Y. 2019/20
Degree course code (?) Degree course track Course unit code Course unit name Credits Year Period Lang. Teacher in charge
IF1839 COMMON SCP3056723 12 3rd Year (2019/20) First
semester
ITA LIVIO TRAINOTTI
IF1839 COMMON SCP7081217 6 3rd Year (2019/20) First
semester
ITA LIVIO TRAINOTTI
IF1839 COMMON SCP7081218 6 3rd Year (2019/20) First
semester
ITA LIVIO TRAINOTTI
IN2374 COMMON INP7078929 9 1st Year (2019/20) Second
semester
ITA GIANFRANCO SANTOVITO