TISO NATASCIA

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Structure Department of Biology
Telephone 0498276302
Qualification Professore associato confermato
Scientific sector BIO/13 - APPLIED BIOLOGY
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Office hours
Wednesday from 12:00 to 13:00 Ufficio Prof. N. Tiso - I Piano Sud - Lab. 09-10 - Centro Interdipartimentale Vallisneri Si prega di concordare l'appuntamento via e-mail (natascia.tiso@unipd.it) o per telefono (049/8276302)
(updated on 29/04/2018 08:26)

Proposals for thesis
Master's thesis topics:

a) Use of zebrafish as a model for human arrhythmogenic cardiomyopathy
b) Use of zebrafish as a model for human mitochondrial disorders
c) Use of zebrafish as a model for the IUGR human condition
d) Analysis of pancreatic development and cell fate in the formation of the endocrine and exocrine compartment in zebrafish
e) Use of zebrafish for the analysis and treatment of human cancer cells

Bachelor's thesis; activities and techniques on display:

a) Practical activities for laboratory management (cleaning of work areas, sterilization of materials, instrumentation calibration and maintenance)
b) Basic techniques in molecular biology (nucleic acid extraction, PCR and genotyping, gel electrophoresis)
c) Embryological techniques on the zebrafish model (collection and manipulation of fertilized eggs, observation of developmental stages, fixation, immuno-histochemistry and in situ hybridization, in vivo and post-mortem observations and documentation by classical and confocal optical microscopy, in bright-field and fluorescence).

Curriculum Vitae
PUBLICATIONS:
64 publications; 3515 citations; 55 cit/article; h-index=25 (Google Scholar: h-index=26)
>70 international and > 30 national presentations

TITLES AND WORK EXPERIENCES
1994: Degree in Biological Sciences, Biomolecular Course - 110/110 cum laude
1996: Official State Exam as Professional Biologist
1997: Visiting Scientist at the University of Toronto, Ontario, Canada
1998: PhD in Genetic Sciences, Univ. of Bologna, Ferrara and Padua
1999-2000: Post-doctorate in Genetics of Hereditary Cardiomyopathies - Univ. Padua
2001-2007: EU Research Collaborator - Model organism: zebrafish (Danio rerio)
2005: Stage at MPI Tuebingen, Germany, Lab. of Nobel Prize Prof. C.N. Volhard
2007-2015: University Researcher - Dept. of Biology - University of Padua
2015-present: Associate Professor - Dept. of Biology - University of Padua

AWARDS AND SCHOLARSHIPS
1997: Telethon annual scholarship for training abroad
1997: "Lions Club Verona Europa" award for arrhythmogenic cardiomyopathy
2001: "Martino Arrigoni" Award for Congenital Cardiopathies, Rotary Club BL
2003: "Alberto Monroy" Award for the MBL course, USA, University of Palermo
2003: L'Oreal-UNESCO Award (on ARVD) for Women and Science in Italy
2003: Howard Hughes Medical Institute Award (MBL Embryology Course 2003) USA
2008-present: Annual Mobility Fellowship Winner, Univ. Padova / Univ. Freiburg Exchange
2013: Poster Prize COB, European Zebrafish Meeting, Barcelona, ​​Spain
2017: Poster Prize EASD-ISG / BCW, 2nd EASD-ISG / BCW Joint Meeting, Dresden, Germany

FUNDING
2002-2003: Young Researcher Funding on: ARVD - University of Padua
2004: EU Research Collaborator II Level, Co-PI EU Project ZF-MODELS
2008: Curator of the Didactic Fund Project (40,000 Euros) - Univ. of Padua
2009: PI - University Project (on: Alagille Syndrome). Euro 60,000 - Univ. of PD
2009-present: holder of DOR funds - Ministry of Education, University and Research
2009: Curator of the Didactic Fund Project (40,000 Euros) - Univ. of Padua
2009: "Large Equipment" Project Curator (Euro 220,000) - Univ. of Padua
2011: Visiting Scientist Project winner (Euro 5000) - Univ. of Padua
2011: Curator of the Didactic Fund Project (86,000 Euros) - Univ. of Padua
2012: PI Unit, Finalized Research Proj. (on: CH) (Euro 59.124) - UniMI
2013: Junior Post-doc Biennial Fellowship (on CH) - (25,000x2 Euros).
2013: Co-PI University Proj. "Optozen" (Euro 8,000) (on: Imaging in ZF) - UniPD
2014: Co-PI University Proj. "TIGRE" (55,000 euros) - UniPD
2015: PI in the project "POLYGON" (50,000 euros), AFM-Telethon, France
2018: Unit Pi, project "SHoCD" (350,000 euros) - Cariparo Foundation, Padova, Italy

TEACHING AND ACADEMIC ACTIVITIES
2007-2011: holder of the course "Molecular Biology of Development - Mod. B", Sc. Sciences, UniPD
2011-present: holder of the course "Model Organisms in Biology", School of Sciences, UniPD
2015-present: holder of the courses "Applied Biology" and "Genetics", School of Psychology, UniPD
Associations: AGI (Italian Genetic Association), EuFishBioMed European Consortium, Science Advisory and Healthcare Advisory Board, GSA (Genetics Society of America), ASBMB, SBMR, European Society for Fish Models in Biology and Medicine.
Reviewer for about 15 international journals with IF and peer review.

WEB SITES:
ZFIN: http://zfin.org/cgi-bin/webdriver?MIval=aa-persview.apg&OID=ZDB-PERS-020116-8
ResearchGate: https://www.researchgate.net/profile/Natascia_Tiso/

Research areas
Research Areas: Human Genetics, Genetics - Developmental Dynamics and Molecular Biology, Zebrafish (Danio rerio) and Human Pathology.

Research lines:

a) Use of zebrafish as a model for human arrhythmogenic cardiomyopathy

b) Use of zebrafish as a model for human mitochondrial disorders

c) Use of zebrafish as a model for the IUGR human condition

d) Analysis of pancreatic development and cell fate in the formation of the endocrine and exocrine compartment in zebrafish

e) Use of zebrafish for the analysis and treatment of human cancer cells

Techniques: production and analysis of transgenic zebrafish lines, biosensors and mutants; classic and confocal microscopy; microinjection of constructs, mRNA and morpholino oligos into zebrafish zygotes; xeno-transplantation of human tumor cells; immunohistochemistry and in situ hybridization on whole embryo; routine techniques in genetics and molecular biology; gene targeting via Crispr/Cas9 technology.

Publications
Most significant publications in the last 5 years:

Loss of cardiac Wnt/β-catenin signalling in Desmoplakin-deficient AC8 zebrafish models is rescuable by genetic and pharmacological intervention.
Giuliodori A, Beffagna G, Marchetto G, Fornetto C, Vanzi F, Toppo S, Facchinello N, Santimaria M, Vettori A, Rizzo S, Della Barbera M, Pilichou K, Argenton F, Thiene G, Tiso N, Basso C.
Cardiovasc Res. 2018 Mar 7. doi: 10.1093/cvr/cvy057.

Tcf7l2 plays pleiotropic roles in the control of glucose homeostasis, pancreas morphology, vascularization and regeneration.
Facchinello N, Tarifeño-Saldivia E, Grisan E, Schiavone M, Peron M, Mongera A, Ek O, Schmitner N, Meyer D, Peers B, Tiso N, Argenton F.
Sci Rep. 2017 Aug 29;7(1):9605. doi: 10.1038/s41598-017-09867-x.

Optical mapping of neuronal activity during seizures in zebrafish.
Turrini L, Fornetto C, Marchetto G, Müllenbroich MC, Tiso N, Vettori A, Resta F, Masi A, Mannaioni G, Pavone FS, Vanzi F.
Sci Rep. 2017 Jun 8;7(1):3025. doi: 10.1038/s41598-017-03087-z.

Sox10 contributes to the balance of fate choice in dorsal root ganglion progenitors.
Delfino-Machín M, Madelaine R, Busolin G, Nikaido M, Colanesi S, Camargo-Sosa K, Law EW, Toppo S, Blader P, Tiso N, Kelsh RN.
PLoS One. 2017 Mar 2;12(3):e0172947. doi: 10.1371/journal.pone.0172947. eCollection 2017.

Monitoring Wnt Signaling in Zebrafish Using Fluorescent Biosensors.
Facchinello N, Schiavone M, Vettori A, Argenton F, Tiso N.
Methods Mol Biol. 2016;1481:81-94. doi: 10.1007/978-1-4939-6393-5_9.

CD271 Down-Regulation Promotes Melanoma Progression and Invasion in Three-Dimensional Models and in Zebrafish.
Saltari A, Truzzi F, Quadri M, Lotti R, Palazzo E, Grisendi G, Tiso N, Marconi A, Pincelli C.
J Invest Dermatol. 2016 Oct;136(10):2049-2058. doi: 10.1016/j.jid.2016.05.116. Epub 2016 Jun 18.

JAG1 Loss-Of-Function Variations as a Novel Predisposing Event in the Pathogenesis of Congenital Thyroid Defects.
de Filippis T, Marelli F, Nebbia G, Porazzi P, Corbetta S, Fugazzola L, Gastaldi R, Vigone MC, Biffanti R, Frizziero D, Mandarà L, Prontera P, Salerno M, Maghnie M, Tiso N, Radetti G, Weber G, Persani L.
J Clin Endocrinol Metab. 2016 Mar;101(3):861-70. doi: 10.1210/jc.2015-3403. Epub 2016 Jan 13.

Venous-derived angioblasts generate organ-specific vessels during zebrafish embryonic development.
Hen G, Nicenboim J, Mayseless O, Asaf L, Shin M, Busolin G, Hofi R, Almog G, Tiso N, Lawson ND, Yaniv K.
Development. 2015 Dec 15;142(24):4266-78. doi: 10.1242/dev.129247. Epub 2015 Nov 2.

Generation and application of signaling pathway reporter lines in zebrafish.
Moro E, Vettori A, Porazzi P, Schiavone M, Rampazzo E, Casari A, Ek O, Facchinello N, Astone M, Zancan I, Milanetto M, Tiso N, Argenton F.
Mol Genet Genomics. 2013 Jun;288(5-6):231-42. doi: 10.1007/s00438-013-0750-z. Epub 2013 May 15. Review.

Wnt activation promotes neuronal differentiation of glioblastoma.
Rampazzo E, Persano L, Pistollato F, Moro E, Frasson C, Porazzi P, Della Puppa A, Bresolin S, Battilana G, Indraccolo S, Te Kronnie G, Argenton F, Tiso N, Basso G.
Cell Death Dis. 2013 Feb 21;4:e500. doi: 10.1038/cddis.2013.32. Erratum in: Cell Death Dis. 2013;4:e597.

List of taught course units in A.Y. 2018/19
Degree course code (?) Degree course track Course unit code Course unit name Credits Year Period Lang. Teacher in charge
PS1082 COMMON PSN1029563 6 1st Year First
semester
ITA NATASCIA TISO
PS1090 COMMON PSO2043598 6 2nd Year First
semester
ITA NATASCIA TISO
SC1166 COMMON SCP3054415 6 3rd Year Second
semester
ITA NATASCIA TISO