First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Science
INDUSTRIAL BIOTECHNOLOGY
Course unit
GENOMICS AND TRANSCRIPTOMICS OF PROKARYOTES AND EUKARYOTES
SCP9088078, A.A. 2019/20

Information concerning the students who enrolled in A.Y. 2019/20

Information on the course unit
Degree course Second cycle degree in
INDUSTRIAL BIOTECHNOLOGY
SC1731, Degree course structure A.Y. 2014/15, A.Y. 2019/20
N0
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Number of ECTS credits allocated 6.0
Type of assessment Mark
Course unit English denomination GENOMICS AND TRANSCRIPTOMICS OF PROKARYOTES AND EUKARYOTES
Website of the academic structure http://biotecnologie.scienze.unipd.it/2019/laurea_magistrale
Department of reference Department of Biology
E-Learning website https://elearning.unipd.it/biologia/course/view.php?idnumber=2019-SC1731-000ZZ-2019-SCP9088078-N0
Mandatory attendance
Language of instruction Italian
Branch PADOVA
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge STEFANO CAGNIN BIO/18

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Educational activities in elective or integrative disciplines BIO/18 Genetics 2.0
Core courses BIO/18 Genetics 3.0
Core courses MED/04 General Pathology 1.0

Course unit organization
Period Second semester
Year 1st Year
Teaching method frontal

Type of hours Credits Teaching
hours
Hours of
Individual study
Shifts
Laboratory 1.0 16 9.0 No turn
Lecture 5.0 40 85.0 No turn

Calendar
Start of activities 02/03/2020
End of activities 12/06/2020
Show course schedule 2019/20 Reg.2014 course timetable

Examination board
Board From To Members of the board
1 GENOMICA E TRASCRITTOMICA DEI PROCARIOTI E DEGLI EUCARIOTI 2019-2020 01/10/2019 27/11/2020 CAGNIN STEFANO (Presidente)
DE PITTA' CRISTIANO (Membro Effettivo)
LANFRANCHI GEROLAMO (Supplente)

Syllabus
Prerequisites: Basic knowledge of molecular and cellular biology and genetics acquired during the Bachelor's degree.
Target skills and knowledge: The course has the objective of presenting the relationship between genome structure and functions also considering techniques for epigenomic analyses. During the course, both simple genomes (bacteria) and complex ones (eukaryotes) will be considered. Traditional gene concept will be revised. During the explanation of the course concepts, techniques used for their definition will be considered. Different applications of next-generation sequencing, qRT-PCR and digital PCR will be described, as well as the methods to study the function of miRNA and long non-coding RNA.
Students will apply in a practical laboratory one technology explained during lessons.
Examination methods: The exam will be an oral conversation to verify the comprehension of the practical experience, the ability of discuss the topics described during lessons and the ability to solve a problem applying techniques discussed in class.
Assessment criteria: During exams, the accuracy of responses and the capacity of the student to correlate related concepts will be evaluated. Moreover, the student will be asked to plan simple experiments to verify a hypothesis. The comprehension of practical exercises will be evaluated during exams.
Course unit contents: • Genomics
Structure of DNA in the regulation of transcription: epigenetic mechanisms in prokaryotes and eukaryotes and an outline of epigenetic regulation in plants. DNA and histone modifications: association with gene transcription (methylation, acetylation, sumoylation, ubiquitination).
Methods for evaluating epigenetic modifications (genomic and post-genomic analyses, analysis of epigenetic markers).
Organization and evolution of genomes. Repetitive DNA, gene families, and comparative genomics.
The mitochondrial genome: organization and duplication.
The transposable elements: DNA transposable elements and retrotransposons in prokaryotes and eukaryotes. Distribution in the genome of transposable elements, their regulation by epigenetic mechanisms. Transposon involvement in contraction and genomic divergence.
• Gene regulation and Transcriptomics
Genomic imprinting.
The transcription regulated by transcription factories: techniques for their study.
Gene expression using qRT-PCR technology, genomic and transcriptomic techniques applied to biotechnological research.
Post-transcriptional regulation of gene expression in prokaryotes and eukaryotes and techniques for their analysis.
CRISPR-CAS innovative technique applied to gene silencing or to induce epigenetic modifications.
Planned learning activities and teaching methods: Teaching activity includes theory lessons and practical sessions.
As far as classroom activities are concerned, students will have the opportunity to present what they have learned in previous courses that have not been studied by their colleagues. The parts of the course that overlap with the course of Molecular and Cellular Biology will be taken for granted. In addition, group activities will be carried out to encourage discussion. Interactive methods will be used to evaluate learning. During practical exercises, the student will set up a qRT-PCR experiment to determine the gene expression of specific target genes.
Additional notes about suggested reading: It is suggested to follow the course during the academic year. The presentation slides used by the teacher and all the articles used to construct the parts of the presentation that cannot be found in the proposed texts will be provided (via moodle).
Textbooks (and optional supplementary readings)
  • Gibson G., Muse S.V., Introduzione alla genomica.. --: Zanichelli, 2004. Cerca nel catalogo
  • Strachan T., Genetics and Genomics in Medicine. --: --, --. Cerca nel catalogo
  • Meneely P., Analisi genetica avanzata.. --: McGraw-Hill, 2012. Cerca nel catalogo

Innovative teaching methods: Teaching and learning strategies
  • Lecturing
  • Laboratory
  • Case study
  • Interactive lecturing
  • Working in group
  • Questioning
  • Problem solving
  • Flipped classroom
  • Peer feedback
  • Auto correcting quizzes or tests for periodic feedback or exams
  • Active quizzes for Concept Verification Tests and class discussions
  • Loading of files and pages (web pages, Moodle, ...)

Innovative teaching methods: Software or applications used
  • Moodle (files, quizzes, workshops, ...)
  • Kahoot

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