First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Science
INDUSTRIAL CHEMISTRY
Course unit
ANALYTICAL TECHNIQUES
SC01123012, A.A. 2019/20

Information concerning the students who enrolled in A.Y. 2019/20

Information on the course unit
Degree course Second cycle degree in
INDUSTRIAL CHEMISTRY
SC1170, Degree course structure A.Y. 2015/16, A.Y. 2019/20
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Number of ECTS credits allocated 6.0
Type of assessment Mark
Course unit English denomination ANALYTICAL TECHNIQUES
Department of reference Department of Chemical Sciences
Mandatory attendance No
Language of instruction Italian
Branch PADOVA
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge SARA BOGIALLI CHIM/01
Other lecturers MARCO FRASCONI CHIM/01

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Core courses CHIM/01 Analytical Chemistry 6.0

Course unit organization
Period Second semester
Year 1st Year
Teaching method frontal

Type of hours Credits Teaching
hours
Hours of
Individual study
Shifts
Laboratory 1.0 12 13.0 No turn
Lecture 5.0 40 85.0 No turn

Calendar
Start of activities 02/03/2020
End of activities 12/06/2020
Show course schedule 2019/20 Reg.2015 course timetable

Examination board
Examination board not defined

Syllabus
Prerequisites: Knowledge of instrumental analysis: mass spectrometry, molecular UV-Vis spectroscopy, fluorescence, electroanalytical chemistry (amperometric methods).
Target skills and knowledge: This course will supply knowledge on planning and managing the most suitable analytical procedures in order to comply with different aims. Screening and confirmatory methods will be described, with different applications in bioanalytic, food, clinical and forensic sciences.
In particular, the course will focus on:
- sample preparation techniques
- screening and confirmatory methods for analysis of biochemical compounds in food, environmental and clinical analysis
- fundamentals of validation protocols
- fundamentals of international guidelines on screening and confirmatory methods
The second part of the course is meant to provide students with a practical overview of current screening analytical techniques with emphasis on bioanalytical applications. At the end of the course the student will be able to understand the instrumental and chemical foundations underlying these techniques and to identify the most appropriate screening and confirmation method to solve an analytical problem.
Examination methods: Oral, two modules
Reports of the activities carried out in lab are mandatory. The oral exam consists of questions on the laboratory activities and on the core topics of the course.
Assessment criteria: Level of understanding of chemical and physical principles underlying the analytical methods.
Knowledge of the fundamentals of instruments, protocols and processes.
Knowledge of the technical and scientific terminology.
Ability to streamline the learned information in order to highlight the most suitable analytical protocols to be used for various applications.
Course unit contents: The course is organised in two modules:
-screening methods (20 h+ 6 h in lab)
-confirmatory methods (20 h+ 6 h in lab).

Screening methods module:
- Introduction to analytical screening methods
- Enzymatic methods. Analytical methods based on the enzyme-substrate reaction. Immobilization of enzymes. Enzymatic sensors.
- Immunological methods. Kinetic and thermodynamic aspect of the antibody-antigen interaction. Immunoenzymatic techniques (ELISA): competitive and non-competitive tests. Optical immunosensors for food, environmental and clinical analysis.
- DNA hybridization and sequencing. Methods to detect gene mutation. DNA microarrays and analysis of gene expression. Aptamers: structure and properties. Fluorescent and electrochemical aptamer-based biosensors.
- Biomimetic sensors. Molecular imprinted polymers: design, preparation and applications.
- Nanomaterials for analytical applications. Integrated nanoparticle–biomolecule hybrid systems for the development of optical sensors.

Laboratory activities:
- Development of an optical sensor for the detection of neurotransmitters.
- Development of an enzymatic assay for the determination of glucose.


Confirmatory methods module:
-hyphenatedsystems for confirmatory analysis
-chromatography coupled to spectrophotometry and mass spectrometry
-sample preparation techniques for solid, liquid and gas
-on-line and off-line techniques
-guidelines for confirmatory methods
-data interpretation
-the analytical reports

Laboratory activities:
-Preparation of samples by using off-line protocols followed by detection with confirmatory systems
-comparative analysis of real samples with screening and confirmatory methods
Planned learning activities and teaching methods: -Lessons with video, simulations, case studies, personal study of selected publications
-Laboratory experiences: sampling, sample treatment and determination of chemicals in environmental matrices by using instrumental methods (LC-UV/FLD; LC-MS; GC-MS, etc.)
Additional notes about suggested reading: -Books
-Lecture notes available on personal websites and moodle.
Textbooks (and optional supplementary readings)
  • Mikkelsen, Corton, Bioanalytical Chemistry. --: John Wiley and Sons, Inc, 2004. Cerca nel catalogo
  • Robert K. Boyd, Trace Quantitative Analysis by Mass Spectrometry 1st Edition with Introduction to Mass Spectrometry Set. --: John Wiley and Sons, Inc, 2012. Cerca nel catalogo

Innovative teaching methods: Teaching and learning strategies
  • Problem based learning
  • Case study
  • Problem solving
  • Loading of files and pages (web pages, Moodle, ...)

Innovative teaching methods: Software or applications used
  • Moodle (files, quizzes, workshops, ...)

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