First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Agricultural Sciences and Veterinary Medicine
BIOTECHNOLOGIES FOR FOOD SCIENCE
Course unit
TRACEABILITY TOOLS FOR SPECIES AUTHENTICATION
AVP5073338, A.A. 2019/20

Information concerning the students who enrolled in A.Y. 2019/20

Information on the course unit
Degree course Second cycle degree in
BIOTECHNOLOGIES FOR FOOD SCIENCE
IF0362, Degree course structure A.Y. 2017/18, A.Y. 2019/20
N0
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Degree course track BIOTECHNOLOGIES FOR FOOD SCIENCE [002LE]
Number of ECTS credits allocated 8.0
Type of assessment Mark
Course unit English denomination TRACEABILITY TOOLS FOR SPECIES AUTHENTICATION
Website of the academic structure https://www.agrariamedicinaveterinaria.unipd.it/
Department of reference Department of Comparative Biomedicine and Food Science
E-Learning website https://elearning.unipd.it/scuolaamv/
Mandatory attendance No
Language of instruction English
Branch LEGNARO (PD)
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge Teacher in charge not defined yet.
Other lecturers MARTINO CASSANDRO AGR/17

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Core courses AGR/07 Agrarian Genetics 8.0

Course unit organization
Period Second semester
Year 1st Year
Teaching method frontal

Type of hours Credits Teaching
hours
Hours of
Individual study
Shifts
Practice 3.0 24 51.0 No turn
Laboratory 1.0 8 17.0 No turn
Lecture 4.0 32 68.0 No turn

Calendar
Start of activities 02/03/2020
End of activities 12/06/2020
Show course schedule 2019/20 Reg.2017 course timetable

Syllabus
Prerequisites: The student needs to know the basic aspects of genomics, with particular reference to the analysis of DNA polymorphisms using molecular markers.

Propedeuticità: None
Target skills and knowledge: Acquire a theoretical and technical knowledge on applied genomics and biotechnologies for the implementation and correct interpretation of molecular tools for marker-assisted selection and diagnostic assays useful for the genetic identification of species, plant varieties, animal breeds and individuals, including the genetic traceability of their food derivatives.
Examination methods: To be defined
Assessment criteria: The student will be evaluated on the basis of theoretical knowledge on structural analysis of genomic DNA, and the ability to use this knowledge to implement diagnostic assays aimed at the genetic traceability of agro-food products of animal and plant origin.
Course unit contents: The five main molecular markers PCR-based used for food traceability and breeding purposes: AFLP, RAPD, SSR, SNP and CAPS

Three generations of sequencing methods: first generation sequencing (Sanger method), NGS (Illumina, SOLiD, 454, Ion Torrent), third generation sequencing (PacBio, MinIon)

Molecular identification of species in fresh and complex food matrixes through nuDNA, cpDNA and mtDNA barcoding. Metabarcoding through NGS techniques. Metagenomics.

Principles of breeding. Definition of pure lines, synthetics, F1 hybrids, clones, multiline landraces. Molecular identification of commercial varieties and landraces and of their food derivatives by means of DNA profiling, exploiting dominant (e.g. RAPD) and codominant markers (e.g. SSR and SNPs)

Genetic similarity statistics, homozygosis and heterozygosis degree for breeding purposes and population analyses. Examples of marker assisted selection (MAS) and marker assisted breeding (MAB) in crop species. Use of molecular markers for EDV (essentially derived variety) and DUS (Distinct, Uniform, Stable) criteria testing

Genetically modified organisms (GMO) and genetic engineering techniques. Three generations of GMO. Principles of EU legislation related to the commercialization of GMO-based food products. GMO Traceability through protein- and DNA-based methods.
Planned learning activities and teaching methods: The course includes:
lectures/practice = 64 hours
Additional notes about suggested reading: The materials used for lessons and practice (powerpoint presentations and scientific publications) will be made available on request.
Textbooks (and optional supplementary readings)