First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Agricultural Sciences and Veterinary Medicine
BIOTECHNOLOGIES FOR FOOD SCIENCE
Course unit
GENOMICS AND BIONFORMATICS
MVN1031739, A.A. 2019/20

Information concerning the students who enrolled in A.Y. 2019/20

Information on the course unit
Degree course Second cycle degree in
BIOTECHNOLOGIES FOR FOOD SCIENCE
IF0362, Degree course structure A.Y. 2017/18, A.Y. 2019/20
N0
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Degree course track BIOTECNOLOGIE PER L'ALIMENTAZIONE [001LE]
Number of ECTS credits allocated 10.0
Type of assessment Mark
Course unit English denomination GENOMICS AND BIONFORMATICS
Website of the academic structure https://www.agrariamedicinaveterinaria.unipd.it/
Department of reference Department of Comparative Biomedicine and Food Science
E-Learning website https://elearning.unipd.it/scuolaamv/
Mandatory attendance No
Language of instruction Italian
Branch LEGNARO (PD)
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge GIANNI BARCACCIA AGR/07
Other lecturers ENRICO MASSIMILIANO NEGRISOLO BIO/05

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Educational activities in elective or integrative disciplines BIO/05 Zoology 4.0
Core courses BIO/13 Applied Biology 6.0

Course unit organization
Period First semester
Year 1st Year
Teaching method frontal

Type of hours Credits Teaching
hours
Hours of
Individual study
Shifts
Practice 2.0 16 34.0 No turn
Laboratory 1.0 8 17.0 No turn
Lecture 7.0 56 119.0 No turn

Calendar
Start of activities 30/09/2019
End of activities 18/01/2020
Show course schedule 2019/20 Reg.2017 course timetable

Syllabus
Prerequisites: The student needs to know the basic aspects of formal genetics and especially of molecular genetics, with particular reference to genes as hereditary units that govern transcriptional and/or translational processes.
Target skills and knowledge: Acquire a theoretical and technical knowledge of genomics for the analysis of genome structure and gene function, with particular reference to agri-food species.
Examination methods: Students will deal with both a written examination (midterm test) and an oral examination (at the end of the course).
Assessment criteria: The student will be evaluated on the basis of theoretical knowledge on structural and functional genomics, and the ability to use this knowledge to plan experiments of genomics applied to specific questions of the agro-food area.
Course unit contents: Classroom teaching: Techniques for the sequencing of genomes; Genome analysis using RFLP and PCR-derived molecular markers; Genetic characterization by means of fingerprinting and genotyping; Genomic DNA sequencing by NGS platforms; Study of gene expression through Northern blot hybridization, RT-PCR and Real-Time PCR; Analysis of the trascriptome: identification of ESTs by RNAseq, SSH and DD techniques, and Microarrays; Analysis of proteome through the construction of 2D-PAG maps; Study of the gene function by using T-RNA and RNAi lines, and over-expression.

Laboratory training: Activities related to DNA isolation, amplification by PCR, restriction and sequencing.
Planned learning activities and teaching methods: The course includes classroom lectures and practical laboratory activities, for a total of 48 hours.
Additional notes about suggested reading: The materials used for frontal lessons and practical activities (pdf files of presentations) of Genomics will be made available on request.

Testo di riferimento: Gianni Barcaccia e Mario Falcinelli (2019) Genetica e Genomica, vol. 3 Genomica e Biotecnologie genetiche (seconda edizione accresciuta e aggiornata). Liguori Editore, Napoli.
Textbooks (and optional supplementary readings)
  • Gianni Barcaccia e Mario Falcinelli, Genetica e Genomica. Vol. 3 Genomica e Biotecnologie genetiche. Napoli: Liguori Editore, 2019. (seconda edizione aggiornata e accresciuta) Cerca nel catalogo