First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Science
BIOLOGY
Course unit
MOLECULAR BIOLOGY (MOD. B)
SCP4068096, A.A. 2019/20

Information concerning the students who enrolled in A.Y. 2018/19

Information on the course unit
Degree course First cycle degree in
BIOLOGY
SC1165, Degree course structure A.Y. 2008/09, A.Y. 2019/20
N0
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Number of ECTS credits allocated 7.0
Type of assessment Mark
Course unit English denomination MOLECULAR BIOLOGY (MOD. B)
Website of the academic structure http://biologia.scienze.unipd.it/2019/laurea
Department of reference Department of Biology
E-Learning website https://elearning.unipd.it/biologia/course/view.php?idnumber=2019-SC1165-000ZZ-2018-SCP4068096-N0
Mandatory attendance
Language of instruction English, Italian
Branch PADOVA

Lecturers
Teacher in charge MARIA EUGENIA SORIANO GARCIA - CUERVA BIO/11

Integrated course for this unit
Course unit code Course unit name Teacher in charge
SCP4068094 MOLECULAR BIOLOGY AND GENETICS (I.C.) MAURO AGOSTINO ZORDAN

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Basic courses BIO/11 Molecular Biology 7.0

Course unit organization
Period First semester
Year 2nd Year
Teaching method frontal

Type of hours Credits Teaching
hours
Hours of
Individual study
Shifts
Laboratory 1.0 16 9.0 No turn
Lecture 6.0 48 102.0 No turn

Calendar
Start of activities 30/09/2019
End of activities 18/01/2020
Show course schedule 2019/20 Reg.2008 course timetable

Examination board
Examination board not defined

Syllabus

Common characteristics of the Integrated Course unit

Prerequisites: Biochemistry Cell Biology
Target skills and knowledge: The students should understand the relationship between organization and function of the molecules such as nucleic acids and protein inside the cell. The course will provide the basic elements to analyze genome expression using molecular genetics tools.
Examination methods: oral or written with open questions and or multiple choice
Assessment criteria: Acquisition of a specific and appropriate language on the proposed field.
Understanding the levels of regulation of genome expression in bacterial and eukaryotic cells

Specific characteristics of the Module

Course unit contents: THE ORIGIN OF THE MOLECULAR BIOLOGY
The nature of genetic material, the double helix, the central dogma

DNA STRUCTURE AND TOPOLOGY
DNA structures (A, B, Z): chemical structure, parameters, stability, alternative local conformations: cruciforms, hairpins, triple helix, unpaired structures, curvature.
DNA topology: Fuller equation and parameters.

TOPOISOMERASES
Function, types, and mechanisms of action.

RNA STRUCTURE
Chemical structure and topology
Chemical modifications, secondary and tertiary structures
Conformation-function correlation

THE GENETIC CODE
Deciphering, structure, and evolution.
Reading and ORF phases.
Amber and Ochre mutants.

ORGANIZATION OF GENETIC MATERIAL
Viruses, bacteria and eukaryotic DNA
Packaging levels
The nucleosome: components, assembly, and post-translational modifications. Distribution and positioning during replication and transcription.
Chromatin: structure, conformation, and functionality. Centromeres and Telomeres
Atypical organizations

ORGANIZATION OF GENETIC INFORMATION
In prokaryotes: operons
In eukaryotes: coding and non-coding regions
Genetic profiles

DNA REPLICATION.
Proposed models
Origin of replication: identification and regulation
Mechanism and phases of the replication process.
Replication machinery
Telomeres and nucleosomal DNA (epigenetics)
The faithfulness of DNA replication

TRANSCRIPTION IN PROCARIOTS.
Transcription unit, RNA polymerase and Phases
Promoters
Closed and open complex: sigma and abortion phase factors.
Extension and termination rho-dependent and independent
Regulation of transcription: Operons classification
Lactose and tryptophan operons

FAGO LAMBDA
Regulation of the lytic and lysogenic cycle

TRANSCRIPTION IN EUKARYOTES.
RNA polymerase phases I, II and III: Characteristics, differences, and functions.
Promoters and transcription factors.
Complex mediator.
Regulation of transcription: Activators, co-activators and silencers. Distal and proximal sites.
Chromatin remodeling and methylation. Genetic imprinting

RNA MATURATION.
Eukaryotes: splicing, categories of introns, splicing mechanisms, Self-splicing;
Catalytic RNA, evolutionary implications; enzymes with RNA components and proteins; small nuclear RNAs.

TRANSLATION.
Ribosomal RNA and tRNA; ribosomes; protein synthesis; starting and elongation factors. Ribosome as a molecular machine.
Modifications of mRNA: polyadenylation and CAP. Problem of eukaryotic regulation as a combinatorial system. Examples of regulation at the level of chromatin modifiers: I’RNA as a regulator, siRNA, miRNA,snRNA.

TECHNIQUES:
Agarose gel electrophoresis
Southern blot
Cloning: restriction enzymes, plasmids and ligases
Polymerase Chain reaction (PCR)
Sequencing techniques
Silencing: siRNA
Acrylamide gel and western blot electrophoresis

LABORATORY ACTIVITY
Analysis of a transposon in the human genome. extraction of DNA from saliva and amplification of the locus PV 92 and study of the polymorphism.
Planned learning activities and teaching methods: Taught lessons and laboratory sessions
Interactive quizzes and presentations on specific topics will contribute to consolidate knowledge and to increase the score obtained in the exam.
Additional notes about suggested reading: An e-learning platform is active where the slides used during lessons and other papers in English are present.
Powerpoint presentations are mostly in English.
Textbooks (and optional supplementary readings)
  • Francesco Amaldi, Piero Benedetti, Graziano Pesole, Paolo Plevani, Biologia Molecolare, Terza Edizione. --: Ambrosiana, 2018. Terza edizione Cerca nel catalogo

Innovative teaching methods: Teaching and learning strategies
  • Lecturing
  • Laboratory
  • Problem based learning
  • Case study
  • Working in group
  • Action learning
  • Problem solving
  • Active quizzes for Concept Verification Tests and class discussions
  • Use of online videos
  • Loading of files and pages (web pages, Moodle, ...)

Innovative teaching methods: Software or applications used
  • Moodle (files, quizzes, workshops, ...)