First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Science
MOLECULAR BIOLOGY
Course unit
MOLECULAR AND CELL BIOLOGY OF PLANTS
SCP8085062, A.A. 2018/19

Information concerning the students who enrolled in A.Y. 2018/19

Information on the course unit
Degree course Second cycle degree in
MOLECULAR BIOLOGY
SC2445, Degree course structure A.Y. 2018/19, A.Y. 2018/19
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Degree course track Common track
Number of ECTS credits allocated 9.0
Type of assessment Mark
Course unit English denomination MOLECULAR AND CELL BIOLOGY OF PLANTS
Website of the academic structure http://biologiamolecolare.scienze.unipd.it/2018/laurea_magistrale_biologiamolecolare
Department of reference Department of Biology
E-Learning website https://elearning.unipd.it/biologia/course/view.php?idnumber=2018-SC2445-000ZZ-2018-SCP8085062-N0
Mandatory attendance
Language of instruction English
Branch PADOVA
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge BARBARA BALDAN BIO/01
Other lecturers LORELLA NAVAZIO BIO/01

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Core courses BIO/01 General Botanics 7.0
Core courses BIO/04 Vegetal Physiology 2.0

Course unit organization
Period First semester
Year 1st Year
Teaching method frontal

Type of hours Credits Teaching
hours
Hours of
Individual study
Shifts
Laboratory 1.0 16 9.0 2
Lecture 8.0 64 136.0 No turn

Calendar
Start of activities 01/10/2018
End of activities 18/01/2019

Examination board
Board From To Members of the board
1 MOLECULAR AND CELL BIOLOGY OF PLANTS 2018-2019 01/10/2018 30/11/2019 BALDAN BARBARA (Presidente)
NAVAZIO LORELLA (Membro Effettivo)
DALLA VECCHIA FRANCESCA (Supplente)

Syllabus
Prerequisites: Students should have already acquired a basic knowledge of Cell Biology, Plant Biology, Biochemistry and Molecular Biology.
Target skills and knowledge: The aim of this course is, on the basis of the knowledge acquired during the first level degree, to study in depth some known subjects and to tackle new plant biology topics from a molecular and cellular point of view.
Examination methods: To verify the acquired knowledge, the exam will be in written form, with open questions on theoretical topics dealt with during the course, as well as questions concerning the practical activity carried out in the laboratory. The active partecipation to the discussions proposed during teaching classrooms will also be considered.
Assessment criteria: Evaluation criteria will be:
- the degree of knowledge, learning and understanding of topics;
- the capability to answer to the proposed questions with critical aptitude and correct scientific language;
- the aptitude to present and discuss topics during the teaching classrooms.
Course unit contents: Ca2+-mediated signal transduction in response to biotic and abiotic stresses in plants: Ca2+, an intracellular second messenger; methods of measuring intracellular Ca2+ concentration; calcium transients and calcium signatures (4h).
Plant hormones (auxins, gibberellins, cytokinins, ethylene, abscissic acid): biosynthesis, actions, transport and developmental effects; signal transduction pathways (16h).
Growth and development: Shoot and root apical meristems: their establishment and maintenance. Determination of the developmental axes and the involved genes. Molecular aspects of lateral organ formation (6h).
Blue light and red light responses: light perception, signal transduction and plant responses to light environmental conditions (6h).
Plant reproductive development: floral meristem development, floral organ identity genes, ABCDE model to explain the flower development; the control of flowering (8h).
Molecular aspects in micro and macro-gametogenesis; self-incompatibility during the pollen-pistil interactions; genes involved in control of double fertilization; embryo, seed and fruit development (14h).
Plant–microorganism interactions: cellular and molecular surveys about mycorrhiza, Rhizobium-Leguminosae symbiosis and plant-Agrobacterium interaction (10h).

16h (1 CFU) of practical work are planned on the following topics:
1) Somatic embryogenesis in the model system Daucus carota, tobacco micropropagation
2) Isolation of protoplasts from plant cell suspension cultures; fluorescence imaging of intracellular compartments
3) Protein extraction and quantification from Arabidopsis thaliana cell cultures stably expressing the calcium-sensitive photoprotein aequorin
4) Analysis of protein expression by SDS-PAGE and immunoblotting
Planned learning activities and teaching methods: The course combines theoretical lessons with laboratory. The lab experience has some main goals: to understand and apply the acquired knowledge to practical activities (comprehension and application of experimental protocols) and to gain ability of evaluation (critical discussion of the obtained results).
Additional notes about suggested reading: All the powerpoint slides used during lectures will be provided on the e-learning platform. Concerning some subjects, teachers will also provide specific papers from specialized literature.
Textbooks (and optional supplementary readings)
  • Lincoln Taiz, Eduardo Zeiger, Ian M. Møller, and Angus Murphy, Plant Physiology and Development. --: Sinauer Associates, 2015. Sixth Edition Cerca nel catalogo
  • Buchanan B, Gruissem W, Jones RL, Biochemistry and Molecular Biology of Plants, 2nd Edition. --: Wiley, 2015. Cerca nel catalogo

Innovative teaching methods: Teaching and learning strategies
  • Lecturing
  • Laboratory
  • Case study
  • Interactive lecturing
  • Working in group
  • Questioning
  • Active quizzes for Concept Verification Tests and class discussions
  • Use of online videos
  • Loading of files and pages (web pages, Moodle, ...)

Innovative teaching methods: Software or applications used
  • Moodle (files, quizzes, workshops, ...)

Sustainable Development Goals (SDGs)
Zero Hunger Responsible Consumption and Production Climate Action Life on Land