First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Science
INDUSTRIAL BIOTECHNOLOGY
Course unit
MOLECULAR SYNTHETIC BIOTECHNOLOGY
SCP7081257, A.A. 2018/19

Information concerning the students who enrolled in A.Y. 2018/19

Information on the course unit
Degree course Second cycle degree in
INDUSTRIAL BIOTECHNOLOGY
SC1731, Degree course structure A.Y. 2014/15, A.Y. 2018/19
N0
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Number of ECTS credits allocated 6.0
Type of assessment Mark
Course unit English denomination MOLECULAR SYNTHETIC BIOTECHNOLOGY
Website of the academic structure http://biotecnologie.scienze.unipd.it/2018/laurea_magistrale
Department of reference Department of Biology
E-Learning website https://elearning.unipd.it/biologia/course/view.php?idnumber=2018-SC1731-000ZZ-2018-SCP7081257-N0
Mandatory attendance
Language of instruction Italian
Branch PADOVA
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge ELISABETTA BERGANTINO BIO/11
Other lecturers PAOLO LAVEDER BIO/18

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Core courses BIO/11 Molecular Biology 3.0
Core courses MED/04 General Pathology 3.0

Course unit organization
Period Second semester
Year 1st Year
Teaching method frontal

Type of hours Credits Teaching
hours
Hours of
Individual study
Shifts
Lecture 6.0 48 102.0 No turn

Calendar
Start of activities 25/02/2019
End of activities 14/06/2019
Show course schedule 2019/20 Reg.2014 course timetable

Examination board
Board From To Members of the board
2 BIOTECNOLOGIA SINTETICA MOLECOLARE 2018-2019 01/10/2018 30/11/2019 BERGANTINO ELISABETTA (Presidente)
LAVEDER PAOLO (Membro Effettivo)
FILIPPINI FRANCESCO (Supplente)
1 BIOTECNOLOGIA SINTETICA MOLECOLARE 2017/2018 01/10/2017 25/11/2018 BERGANTINO ELISABETTA (Presidente)
DE BERNARD MARINA (Membro Effettivo)
FILIPPINI FRANCESCO (Supplente)

Syllabus
Prerequisites: Basic knowledge of molecular biology (Molecular Biology of prokaryotic and eukaryotic organisms); principles of genetic engineering.
Target skills and knowledge: Subjects of the course are principles and techniques of genetic manipulation of organisms, with particular regards to the production of useful molecules and recombinant proteins in both well-established and innovative systems. Different host systems will be described, as used in the lab-scale but also in their extension to the industrial application.
Principles, techniques of production and applications of antibodies and antibody fragments.
Examination methods: Written exams with open questions.
Assessment criteria: Verification of the acquisition of the knowledge of the contents and of an appropriate and specific lexicon on the topics of the course.
Course unit contents: First part (Synthetic biology)
- Protein expression in E.coli: analysis, design and modification of factors affecting expression of cloned genes.
- Protein expression in yeast: Saccharomyces cerevisiae vs Pichia pastoris, similarities and peculiarities. The problem of protein glycosylation.
- Protein expression in insect cells: elements of the molecular biology of baculovirus, engineering of its genome; bacmids; ‘humanized’ insect cells.
- Genome editing: meganucleases, ZF-nucleases, TALEN and CRISPR/Cas technology. Cre and FLP recombinases.
- Protein engineering: rational-, semirational-design and directed evolution. Examples in biocatalysis, bioremediation, useful proteins for the research and in biomedicine. Protein synthesis in cell-free systems.
- Metabolic engineering in microorganisms and animal cells (CHO).

Second part (Recombinant antibodies)
- Production of monoclonal, chimeric, iperchimeric and human antibodies. Applications.
- Production of antibody fragments.
- Application of phage-display technique to the selection of antibody fragments.
- Design of antibodies for therapeutic use (antibodies conjugated to toxins or anti-tumoral drugs).
Planned learning activities and teaching methods: Classroom lectures.
Additional notes about suggested reading: Powerpoint presentations of lessons, articles and specific reviews will be accessible on the web at the address:
http://elearning.scienze.unipd.it
Textbooks (and optional supplementary readings)
  • Glick, Pasternak, Patten, Molecular Biotecnology – principles and applications of recombinant DNA - 4th edition. --: ASM press - Ed. italiana distribuita da Zanichelli, --. Cerca nel catalogo

Innovative teaching methods: Teaching and learning strategies
  • Lecturing
  • Working in group
  • Questioning
  • Peer feedback
  • Peer assessment
  • Active quizzes for Concept Verification Tests and class discussions
  • Loading of files and pages (web pages, Moodle, ...)

Innovative teaching methods: Software or applications used
  • Moodle (files, quizzes, workshops, ...)
  • Kahoot / Poll Everywhere

Sustainable Development Goals (SDGs)
Good Health and Well-Being Decent Work and Economic Growth Industry, Innovation and Infrastructure Responsible Consumption and Production Climate Action