First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Science
INDUSTRIAL BIOTECHNOLOGY
Course unit
LARGE-SCALE CELL CULTURES AND BIOMOLECULES PRODUCTION
SCN1037574, A.A. 2018/19

Information concerning the students who enrolled in A.Y. 2018/19

Information on the course unit
Degree course Second cycle degree in
INDUSTRIAL BIOTECHNOLOGY
SC1731, Degree course structure A.Y. 2014/15, A.Y. 2018/19
N0
bring this page
with you
Number of ECTS credits allocated 8.0
Type of assessment Mark
Course unit English denomination LARGE-SCALE CELL CULTURES AND BIOMOLECULES PRODUCTION
Website of the academic structure http://biotecnologie.scienze.unipd.it/2018/laurea_magistrale
Department of reference Department of Biology
E-Learning website https://elearning.unipd.it/biologia/course/view.php?idnumber=2018-SC1731-000ZZ-2018-SCN1037574-N0
Mandatory attendance
Language of instruction English
Branch PADOVA
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge CHIARA RAMPAZZO BIO/06

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Educational activities in elective or integrative disciplines CHIM/08 Pharmaceutical Chemistry 3.0
Core courses BIO/06 Comparative Anatomy and Citology 5.0

Course unit organization
Period Second semester
Year 1st Year
Teaching method frontal

Type of hours Credits Teaching
hours
Hours of
Individual study
Shifts
Lecture 8.0 64 136.0 No turn

Calendar
Start of activities 25/02/2019
End of activities 14/06/2019

Examination board
Board From To Members of the board
7 PRODUZIONI INDUSTRALI DI CELLULE E BIOMOLECOLE 2018-2019 01/10/2018 30/11/2019 RAMPAZZO CHIARA (Presidente)
MOGNATO MADDALENA (Supplente)
6 PRODUZIONI INDUSTRIALI DI CELLULE E BIOMOLECOLE 2017/2018 01/10/2017 25/11/2018 RAMPAZZO CHIARA (Presidente)
GANDIN VALENTINA (Membro Effettivo)
MOGNATO MADDALENA (Supplente)

Syllabus
Prerequisites: Students are expected to have knowledge of cellular and molecular biology and of biochemistry.
Target skills and knowledge: The aims of the course is to provide students with the basic knowledge on large-scale mammalian cell cultures in the upstream and downstream phases of the industrial production process. The course is designed to give students the necessary theoretical tools and information to be able to know:
1) the procedures to isolate a clonal mammalian cell line for the overproduction of a specific biomolecule,
2) how to select the right bioreactor based on the properties of the biomolecule to be produced,
3) how to improve cellular vitality in a bioreactor,
4) how to improve the process productivity by optimizing medium composition and cellular metabolism,
5) how to expand in large scale embryonal or adult stem cell cultures for cell therapy applications.
6) which are the current biomolecules of pharmaceutical interest and how they can be produced.
Examination methods: The final exam will be oral and organized in two parts. Students will be evaluated collegially by both professors on the knowledge acquired on all the material proposed during the course.
First part (5 CFU) is described in the course contents at section 1, 2 and 3. The second part (3 CFU) is described in the course content at section 4. The final grade is expressed as a weighted average between the two parts.
Assessment criteria: The purpose of the oral examination is to assess the student knowledge on all the topics illustrated in the programme of the lecture course.
The criteria to evaluate student’s knowledge are:
1) understanding of the topics covered in the course
2) critical thinking skills
3) completeness of the acquired knowledge
4) capacity for analysis and synthesis
Course unit contents: 1) Overview of the biopharmaceutical industry. Upstream and downstream processes. GMP/GLP regulatory requirements for processing biopharmaceuticals. Lab/pilot scale process to implement full manufacturing scale. Consistency and robustness in a fermentation process. Large scale mammalian cell culture. How to calibrate oxygen, pH, nutrients and metabolites, cell density and viability in the bioreactor. Design of cell culture medium without serum and with low content of proteins.

2) Design of large scale cell culture process for mammalian cell culture. How to improve cell viability in a process. Cell line engineering techniques and common host cell lines used. Bioreactor operation mode: batch, fed batch, continuous and perfusion culture. Selection of bioreactor type (spinner flask, stirred tank). Attachment systems for cell cultivation in adhesion (plates, roller bottle, and stacked plate system) packed bed bioreactor, microcarriers, fluidized bed bioreactor, hollow fiber and wave bioreactor. Perfusion systems for cell cultivation (hollow fiber, spin filter, acoustic cell separation, alternating tangential flow (ATF) system). Scaffold and matrix in bioreactors. Expression of cloned proteins in mammalian cells, e.g. interferon and insulin. Large scale production of monoclonal antibodies and their use. Vaccine process development in mammalian cells and manufacturing of vaccines.

3) Large scale Embryonic and adult stem cell cultures and their application in cell therapy.

4) Biomolecules of pharmaceutical interest. Cytokines: interleukins and interferons. Hormons: insulin and growth hormone. Enzymes: tissue plasminogen activator and DNase. Erithropoietin. Heparin. Monoclonal antibodies: pharmaceutical and therapeutic properties. Monoclonal antibody-based drugs for antitumor, immunosuppressive, antithrombotic, antiviral, antiasthma and antiangiogenic therapies.
Planned learning activities and teaching methods: The course is organized in lectures and group work:
1) The knowledge provided by the program is presented in the lectures using ppt, images, diagrams and videos. The teaching is interactive, with questions and presentation of research studies (based on articles and reviews) to promote critical thinking and discussion in the classroom.
2) the group activities consist of problem solving and flipped classroom exercises.
Additional notes about suggested reading: As supplementary materials, power point slides of all lectures, research paper and review will be available on https://elearning.unipd.it/biologia/. Recent scientific publication on international journal useful for the understanding of specific topics will be suggested.
Textbooks (and optional supplementary readings)
  • S.S.Ozturk AND W.S Hu, CELL CULTURE TECHNOLOGY FOR PHARMACEUTICAL AND CELL-BASED THERAPIES. --: TAYLOR AND FRANCIS, 2006. Cerca nel catalogo
  • M. Butler, CELL CULTURE AND UPSTREAM PROCESSING. --: TAYLOR AND FRANCIS, 2007. Cerca nel catalogo

Innovative teaching methods: Teaching and learning strategies
  • Lecturing
  • Problem based learning
  • Interactive lecturing
  • Working in group
  • Questioning
  • Problem solving
  • Flipped classroom
  • Use of online videos

Innovative teaching methods: Software or applications used
  • Moodle (files, quizzes, workshops, ...)

Sustainable Development Goals (SDGs)
Good Health and Well-Being Industry, Innovation and Infrastructure