First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Medicine
Course unit
MEP5070485, A.A. 2019/20

Information concerning the students who enrolled in A.Y. 2018/19

Information on the course unit
Degree course Second cycle degree in
ME2193, Degree course structure A.Y. 2015/16, A.Y. 2019/20
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Number of ECTS credits allocated 10.0
Type of assessment Mark
Department of reference Department of Pharmaceutical and Pharmacological Sciences
E-Learning website
Mandatory attendance
Language of instruction English
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Teacher in charge SUSANNA MANDRUZZATO MED/04
Other lecturers PAOLA BRUN MED/07

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Core courses MED/07 Microbiology and Clinical Microbiology 6.0
Core courses MED/04 General Pathology 4.0

Course unit organization
Period First semester
Year 2nd Year
Teaching method frontal

Type of hours Credits Teaching
Hours of
Individual study
Laboratory 1.0 15 10.0 No turn
Lecture 9.0 72 153.0 No turn

Start of activities 30/09/2019
End of activities 18/01/2020
Show course schedule 2019/20 Reg.2015 course timetable

Examination board
Board From To Members of the board
4 Commissione a.a. 2019/20 02/12/2019 30/09/2020 MANDRUZZATO SUSANNA (Presidente)
RICHTER SARA (Membro Effettivo)
BRUN PAOLA (Supplente)
3 Commissione a.a. 2018/19 03/12/2018 30/11/2019 MANDRUZZATO SUSANNA (Presidente)
RICHTER SARA (Membro Effettivo)
BRUN PAOLA (Supplente)

Prerequisites: Diagnostic microbiology

Basic microbiology: general properties of bacteria, viruses, mycetes, protozoa; basic concepts in immunology and molecular biology.

Molecular Immunology

Principles of Immunology
Target skills and knowledge: Diagnostic microbiology

This course is concerned with diagnostic microbiology in order to control microorganisms within the environment and hence from contaminating the biological product.
The main goal of this course is provide theoretical and practical information on pharmaceutical microbiology, including main diagnostics procedures, protocols and guidelines for the identification and characterisation of micro-organisms, elements that are necessary for the microbiological quality controls during development and manufacturing of biotechnological products.

Molecular Immunology

This course has a specific reference to the biotechnological research in the field of immunology and pharmacology. The student will obtain a number of information upon which he/she will be able to build a solid knowledge in these fields.
Examination methods: Diagnostic microbiology

The final exam consists of multiple choice questions.
The teacher will propose during the course different practical and theoretical activities that will contribute to the final grade .

Molecular Immunology

The final exam consists of multiple choice questions, or, in selected cases, oral exam.
Assessment criteria: Diagnostic microbiology

Written exam: 70% of the final mark.
Evaluation of the other activities (learning activities, participation during the lessons and practical activity report): 30% of the final mark.

Molecular Immunology

Written exam: 100% of the final mark.
Course unit contents: Diagnostic microbiology

Microbiology and pharmaceuticals
GMP and regulations
Laboratory management and design
Microbiological culture media
Microbiology laboratory techniques
Bioburden determination
Specified and objectionable microorganisms
Microbial identification
Assessment of pharmaceutical water systems
Endotoxin and pyrogen testing
Sterilization and sterility assurance
Biological indicators: Measuring sterilization
Antibiotics and preservatives
Cleaning and disinfection
Cleanrooms and environmental monitoring
Rapid microbiological methods
Risk assessment
Manufacturing and validation
Microbiological data
Auditing the microbiology laboratory
Microbiological challenges to the pharmaceuticals and healthcare
Mycetes: general concepts and guidelines for identification and culture
Biofilms and Quorum Sensing
Study of complex microbial commuities

Molecular Immunology

Characteristics of the immune system. Innate and adaptive immunity. Identification of the pathogen by the cells of the innate system: PAMP and PRR. Toll-like receptors. Origin of the cells of the immune system. Recruitment of the leukocytes to the infection site. Natural killer cells and their role in the immune system.
Major histocompatibility complex: gene organization, protein structure and biological function. HLA typing.
Antibody structure. Monoclonal and polyclonal antibodies, generation of monoclonal antibody and their use in the lab and in the clinic. Evolution of monoclonal antibody. Monoclonal antibodies as drugs. B cell activation and antibody production: signal transduction and functional responses.
The innate response to viral infections.
Immunological tolerance: T Cell Development, Repertoire Selection and Immune Self Tolerance.
Transplantation immunology, Types of tranplant. Immune responses to allografts. Recognition of antigens, activation of alloreactive lymphocytes. Patterns and mechanisms of allograft rejection. Immunosuppressive drugs.
Maturation of T lymphocytes and signals for T cell activation. Balancing T cell activation and control. Functional responses of T lymphocytes: Th1, Th2, Th17. Decline of T cell responses.
Autoimmunity and autoimmune diseases. Mechanisms of autoimmune diseases.
Immunity to tumors. Immune responses to tumors. Tumor antigens. Evasion of immune responses by tumors. Immunotherapy for tumors. Recent advances in the immunotherapy of cancer.
Flow cytometry: principles and application in immunology
Planned learning activities and teaching methods: Diagnostic microbiology

The course is organised in lectures (40 hours) and laboratory activities (15 hours).
a. Frontal Lectures. The lectures, given by the teacher, will provide knowledge on the main diagnostics procedures and protocols, for identification and characterisation of micro-organisms, required for the microbiological quality controls during development and manufacturing of biotechnological products.
b. Learning activities, participation during the lessons. Students will be asked for specific learning activities during the frontal lectures.
c. Laboratory activities. The laboratory activities will include the execution of diagnostic assays under the teacher’s guide and a practical activity report. For the activities in b) and c) each student will receive a mark (that will contribute to 30% of the final mark).

Molecular Immunology

The course is organized in lectures (36 hours), including technical practical lessons.
a. Frontal lectures. The lectures, given by the teacher, are aimed at elucidating all the molecular mechanisms present in the program, with the help of the slides and a close interaction between the teacher and the students.
b. Technical practical lessons. During these lessons students will be given examples of several multiple choice question, similar to those of the final exam, that will be discussed between students and teacher.
Additional notes about suggested reading: Diagnostic microbiology

A copy of the power point slides will be handled to the students on Moodle.

Molecular Immunology

Copy of the slides will be given to the students on the Moodle platform.
Textbooks (and optional supplementary readings)
  • Tim Sandle, Pharmaceutical microbiology. Essentials for quality assurance and quality controls. Cambridge UK: Woodhead Publishing Elsevier, 2015. Cerca nel catalogo
  • Connie R. Mahon MS MT(ASCP) CLS (Author), Donald C. Lehman EdD MT(ASCP) SM(NRM) (Author), George M, Textbook of Diagnostic Microbiology, 5e. Mahon, Textbook of Diagnostic Microbiology, 2014. Cerca nel catalogo
  • Abbas, Abul K.; Pillai, Shiv, Cellular and molecular immunologyAbul K. Abbas, Andrew H. Lichtman, Shiv Pillai. Philadelphia [etc.]: Elsevier, 2018. Cerca nel catalogo
  • Janeway, Charles; Weaver, Casey T.; Mowat, Allan; Murphy, Kenneth, Janeway's Immunobiology. 9. ed. / Kenneth Murphy: Casey Weaver, with contributions by Allan Mowat ... [et al.], with acknowledgment to Charles A. Janeway Jr. ... [et al.]. - New York, London, Garland Science, --. Cerca nel catalogo
  • Parham Peter, The immune systemPeter Parham. New York: Taylor & Francis, 2015. Cerca nel catalogo
  • Abbas, Abul K.; Pillai, Shiv, Cellular and molecular immunologyAbul K. Abbas, Andrew H. Lichtman, Shiv Pillai. Philadelphia [etc.]: Elsevier, --. Cerca nel catalogo

Innovative teaching methods: Teaching and learning strategies
  • Laboratory
  • Auto correcting quizzes or tests for periodic feedback or exams

Innovative teaching methods: Software or applications used
  • Moodle (files, quizzes, workshops, ...)

Sustainable Development Goals (SDGs)
Good Health and Well-Being Quality Education Responsible Consumption and Production