First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Agricultural Sciences and Veterinary Medicine
Course unit
MVO2045628, A.A. 2019/20

Information concerning the students who enrolled in A.Y. 2018/19

Information on the course unit
Degree course Second cycle degree in
IF0362, Degree course structure A.Y. 2017/18, A.Y. 2019/20
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Number of ECTS credits allocated 6.0
Type of assessment Mark
Course unit English denomination PHYLOGENETIC ANALYSIS OF FOOD ANIMALS
Website of the academic structure
Department of reference Department of Comparative Biomedicine and Food Science
E-Learning website
Mandatory attendance No
Language of instruction Italian
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Other lecturers TOMASO PATARNELLO BIO/05

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Core courses BIO/13 Applied Biology 6.0

Course unit organization
Period First semester
Year 2nd Year
Teaching method frontal

Type of hours Credits Teaching
Hours of
Individual study
Practice 1.0 8 17.0 No turn
Lecture 5.0 40 85.0 No turn

Start of activities 30/09/2019
End of activities 18/01/2020
Show course schedule 2019/20 Reg.2017 course timetable

Prerequisites: The student must know the basic techniques of molecular biology. He needs also a suitable preparation to use the computer, acquired by attending the Bioinformatics course of the first year.
Pre-requisites: None
Target skills and knowledge: The student will learn the evolutionary processes that generate the diversity of species. She/he will learn the theoretical basis for the study of phylogenetic relationships among species of food interest, as well as the use of the software needed to produce phylogenetic trees. The student will learn to know the ichthyic edible species (especially crustaceans and molluscs). She/he will learn how to get the proper identification, to the species level, of complete animal samples or portions of them, by applying a multidisciplinary approach that combines techniques of classical zoology, molecular biology and bioinformatics.
Examination methods: Written and oral examination
Assessment criteria: The student will be evaluated on the basis of the knowledge and the ability to use molecular and computer tools needed to identify, at species-level, the different animal taxa.
Course unit contents: Theoretical part
Principles of population genetics. Concepts and different definitions of population. The Hardy-Weinberg equilibrium (HWE) as the fundamental theorem of population genetics. The evolutionary forces that control the genetic variability. The study of the population genetics of a species: methods and tools. Molecular markers for population genetics. The key markers: advantages, disadvantages and applications. The microsatellite markers. Most common software for population genetics: applications and limits.
Principles of biological systematics. Methods of biological classification: theoretical aspects and applications relating to edible species.
Phylogenetic reconstruction. Main methods used in phylogenetic reconstructions: theory, algorithms, and limits. Molecular identification of species: approaches and markers. The edible molluscs: anatomy, natural history and systematics. The edible crustaceans: anatomy, natural history and systematics.
Dissection of Mollusks of food interest. identification of edible molluscs through the use of dichotomous keys based on morphological and anatomical characters.
Dissection of crustaceans of food interest. Identification of edible crustaceans through the use of dichotomous keys based on morphological and anatomical characters.
Bioinformatic Laboratory:
The program Genepop. The different commands of Genepop. Analysis of molecular data from the genotyping of microsatellite loci. Mechanisms of differentiation of populations. Analysis of various data sets with Genepop.
Using Excel for the calculation of Hardy-Weinberg equilibrium and the associated statistical test. Genalex: an additional tool of Excel for calculating descriptive statistics of population genetics.
Databases used in phylogenetic reconstructions. Genetic and genomic banks and their use.
Analysis of electropherograms and construction of the consensus sequences. Molecular identification of species of molluscs and crustaceans used as food. The DNA barcoding.
Phylogenetic reconstruction. Phylogeny of the species of mollusks and crustaceans based on data sets produced by the students. The use of phylogenetic programs.
Planned learning activities and teaching methods: The course includes:
lectures = 24 hours
laboratory = 8 hours
bioinformatics laboratory = 16 hours
Additional notes about suggested reading: The material used for lectures (slides) is made available on the Moodle platform (

Office hours: daily by appointment with the teacher.
Textbooks (and optional supplementary readings)
  • Brusca R.C., Brusca G.J., Invertebrati. Bologna: Zanichelli, 1996. Edizione italiana della prima edizione Inglese Cerca nel catalogo
  • Brusca R.C. ET AL., Invertebrates. Sunderland, MA: Sinauer, 2016. third edition Cerca nel catalogo
  • Ferraguti M., Castellacci C. (a cura di), Evoluzione. Modelli e processi. --: Pearson, 2011. Cerca nel catalogo