First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Medicine
PHARMACY
Course unit
MICROBIOLOGY AND INFECTIOUS DISEASE PROPHYLAXIS
MEP8083117, A.A. 2019/20

Information concerning the students who enrolled in A.Y. 2018/19

Information on the course unit
Degree course 5 years single cycle degree in
PHARMACY (Ord. 2018)
FA1732, Degree course structure A.Y. 2018/19, A.Y. 2019/20
N0
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Number of ECTS credits allocated 8.0
Type of assessment Mark
Course unit English denomination MICROBIOLOGY AND INFECTIOUS DISEASE PROPHYLAXIS
Department of reference Department of Pharmaceutical and Pharmacological Sciences
Mandatory attendance No
Language of instruction Italian
Branch PADOVA
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge IGNAZIO CASTAGLIUOLO MED/07

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Basic courses MED/07 Microbiology and Clinical Microbiology 8.0

Course unit organization
Period Second semester
Year 2nd Year
Teaching method frontal

Type of hours Credits Teaching
hours
Hours of
Individual study
Shifts
Lecture 8.0 64 136.0 No turn

Calendar
Start of activities 02/03/2020
End of activities 12/06/2020
Show course schedule 2019/20 Reg.2018 course timetable

Examination board
Board From To Members of the board
1 Commissione a.a. 2019/2020 02/12/2019 30/09/2020 CASTAGLIUOLO IGNAZIO (Presidente)
BRUN PAOLA (Membro Effettivo)

Syllabus
Prerequisites: To properly attend to microbiology course students should have basic knowledge of biochemistry, cellular biology, anatomy and physiology mainly of the immune system and cells involved in inflammatatory processes.
Target skills and knowledge: Students will acquire the following knowledges:
- structure and replicative cycle of viruses, bacteria, protozoa and fungi;
- laboratory techniques used to culture and characterize viruses, bacteria, protozoa and fungi;
- principles of bacterial genetic;
- the kind of interaction between the microbe and pluricelluar host; the mechanisms of microbe-induced diseases; mechanisms of anti-microbial defense active in the human body;
- molecular targets of anti-bacteral, anti-viral, anti-protzoari and anti-fungal grugs; moecular mechanisms of onset and evolution of resistance to antimicrobial drugs;

- meaning of prophylaxis of infectious diseases and its application to the population;
- characteristics of vaccines in use;
- procedures of disinfection and sterilization;
- microbiological controls required in the pharmaceutical industry (sterility test, LPS quantification);

- main bacteria, viruses, fungi, protozoa associated to human diseases of skin, respiratory tract, urinary tract and reproductive organs, nervous system, gastrointestinal system, cardiovascular and immune system;
Examination methods: The final test will be administered as open questions: 6 for microbiology and 3 for prophylaxis of infectious diseases.

It will be possible to discuss the test to examine specific issues that have not been properly afddressed by the students.
Assessment criteria: The final exam will aim to determine whether the student has acquired knowledge of microbial structure and physiology as well regarding the mechanisms causing diseases and the molecular targets of antimicrobial drugs. In addition, the test will assess whether the student has acquired the competences regarding the onset of resistance to antimicrobial drugs and associated undesidered effects due to dismicrobisms to properly support the costumers in their correct use. Moreover, the test will investigate whether the student has acquired the knowledge of the correct procedures to prevent infectious diseases spread in the population.
Course unit contents: - Structure of procariotic cell. Grow curve of bacterial culture. Bacterial methabolism and O2/CO2 requirement.
- Criteria to classify bacteria.
- Structure and biological relevance of bacterial spores.
- Techniques to study bacteria: fixatives, simple - differential
- special staining procedures; bacterial growth media (broth and agar medium) and selective/differential/enrichment medium. Strategies to isolate, quantify and identify bacteria in a sample.
- Organizzation of genetic material of bacteria, mechanisms of gene expression control. Mechanisms responsible for gnetic plasticity of bacteria. Meccanisms of genetic material exchange amning bacteria. Plasmids: structure, biological relevance.
- Host-microbe interaction: commensals, parassites.
- Normal microbial population in human body, role in physiological conditions (microbiota as a new organ) and role of dysbiosis in onset of human diseases.
- Natural defences of the organism against infective agents (innate immunity). Adapive immune responses to infectioous agents.
- Virulence
- Mechanisms of bacterial pathogenicity. Bacterial esotoxins: classification, strucutre, mechanism of action, therapeutic application.
- Bacterial endotoxins: structure, mechanism of action, cellular receptors, biological effects, quantification tests and procedures to remove endotoxins contamination.
- Relevant pathogens: H. pylori, N. meningitidis, M. tubercolosis, Streptococchi, Stafilococchi, Enterobatteriacee, Pseudomonas aeruginosa, C. difficile, C. tetani, C. botulinum, Ricketsiae, clamidie.
-Antibiotics: classification, molecular target, antibiotic sensitivity assessment. Antibiotic resistance: clinical relevance, molecular mechanisms, mechanisms of spread.
- Probiotics

-Virus: classification, structure, infection of host cell, replication, effects of viral infections at cellular level and host level
-Human pathogens: influenza virus, herpes viruses, hepatitis viruses, HIV, HPV, West Nile virus, Ebola virus.
-Antiviral drugs: classification, molecular target, resistance development.

-Fungi: classification, cellular structure, cell cycle. Drugs for fungal infections. Fungal infections of the skin and systemic.
-Protozoa: classification, cellular strucuture, cell cycle of protozoa relevant to human diseases: plasmodium malariae, toxoplasma gondii, amebiasi.
-Pediculus humanus.

Epidemiologic chain: reservoirs, source of infections; vectors andvehicles of infective agents. Modality of infectious agents transmission. Modality of entry and exit of pathogens from the human host.

Sterilization: definition. By mean of physical or chemical agents. Disinfection: definition. By mean of physical and chemical agents. Disinfection using chemical agents. Pasteurization and tyndallitation. Pest control.

Active immunoprofilaxis: inactivated vaccines, attenuated vaccines; nucleic acids vaccines. Routes of vaccines administration. Adverse effects of vaccines. Compulsory vaccines in Italy.
Passive immunoprofilaxis: human serum and immunoglobulins. Eterologous serum.
Planned learning activities and teaching methods: The teacher will give class lections having as support slides and videos.
Additional notes about suggested reading: Students will use one of the textbook suggested by the teacher. They will also study in deep specific topics taking advantage of materials provided by the teacher (i.e. Review from international journals). The teacher will provide the slides used for the lectures and links to relevant video available on line.
Textbooks (and optional supplementary readings)
  • Carlone, Pompei, Microbiologia farmaceutica. --: EdiSES, 2012. per studio Cerca nel catalogo
  • Madigan, Martinko, Stahl, Clark, Brock - Biologia dei microrganismi. --: Pearson, 2012. primo volume - per studio Cerca nel catalogo
  • Murray PR, Rosenthal KS, Pfaller MA, Microbiologia Medica settima edizione. --: Masson, 2013. per consultazione Cerca nel catalogo
  • C. Meloni, G. Pelissero, IGIENE.. --: Casa Editrice Ambrosiana, --. per studio Cerca nel catalogo
  • S. Barbuti, G.M. Fara, G. Giammanco, A. Carducci, M.A. Coniglio, D. D'Alessandro, M.T. Montagna, M.L, Igiene. --: Monduzzi Editore, --. per studio Cerca nel catalogo
  • F. Auxilia, M. Pontello, Igiene e Sanità Pubblica. I° e II° volume. --: Piccin, Padova, --. per consultazione Cerca nel catalogo

Innovative teaching methods: Teaching and learning strategies
  • Lecturing
  • Interactive lecturing
  • Questioning

Innovative teaching methods: Software or applications used
  • Moodle (files, quizzes, workshops, ...)
  • Kaltura (desktop video shooting, file loading on MyMedia Unipd)
  • Camtasia (video editing)

Sustainable Development Goals (SDGs)
Good Health and Well-Being