First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Science
BIOLOGY
Course unit
MOLECULAR BIOLOGY (MOD. B)
SCP4068096, A.A. 2018/19

Information concerning the students who enrolled in A.Y. 2017/18

Information on the course unit
Degree course First cycle degree in
BIOLOGY
SC1165, Degree course structure A.Y. 2008/09, A.Y. 2018/19
N0
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Number of ECTS credits allocated 7.0
Type of assessment Mark
Course unit English denomination MOLECULAR BIOLOGY (MOD. B)
Website of the academic structure http://biologia.scienze.unipd.it/2018/laurea
Department of reference Department of Biology
E-Learning website https://elearning.unipd.it/biologia/course/view.php?idnumber=2018-SC1165-000ZZ-2017-SCP4068096-N0
Mandatory attendance
Language of instruction Italian
Branch PADOVA

Lecturers
Teacher in charge MARIA EUGENIA SORIANO GARCIA - CUERVA BIO/11

Integrated course for this unit
Course unit code Course unit name Teacher in charge
SCP4068094 MOLECULAR BIOLOGY AND GENETICS (I.C.) MAURO AGOSTINO ZORDAN

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Basic courses BIO/11 Molecular Biology 7.0

Course unit organization
Period First semester
Year 2nd Year
Teaching method frontal

Type of hours Credits Teaching
hours
Hours of
Individual study
Shifts
Laboratory 1.0 16 9.0 No turn
Lecture 6.0 48 102.0 No turn

Calendar
Start of activities 01/10/2018
End of activities 18/01/2019
Show course schedule 2019/20 Reg.2008 course timetable

Examination board
Examination board not defined

Syllabus

Common characteristics of the Integrated Course unit

Prerequisites: Biochemistry Cell Biology
Target skills and knowledge: The students should understand the relationship between organization and function of the molecules such as nucleic acids and protein inside the cell. The course will provide the basic elements to analyze genome expression using molecular genetics tools.
Examination methods: oral or written with open questions and or multiple choice
Assessment criteria: Acquisition of a specific and appropriate language on the proposed field.
Understanding the levels of regulation of genome expression in bacterial and eukaryotic cells

Specific characteristics of the Module

Course unit contents: The origin of molecular biology.
The nature of genetic material, the double helix, the central dogma
Structures of DNA
DNA A, B, Z, phisycal parameters, local alternative conformations, cruciforms, curved DNA, DNA Topology and Topoisomerases.
Structures of RNA
tRNA, rRNA, the genetic code and amber suppressors.
DNA Replication
The replisome, DNA Polymerases,replication fidelity, proofreading activity, replication of circular and linear DNA, telomeres,
Models of replication, origins of replication in bacterial and eukaryotic cells.
RNA Transcription in Bacteria
RNA Polymerase, subunits, inhibitors, mutants, closed and open complex, promoters, sigma factor, phage lambda regulation, The operon, lac operon, trp operon.
Trascription in eukaryotic cells. RNA Polimerases I, II, III. Promoters, enhancers, general transcripion factors, the mediator, transcriptional activators. the combinatorial control of gene expression.
RNA Processing
Spicing, alternative splicing, cathalytic RNA. RNA world, regulation of gene expression by RNA.
Translation
RNA of the ribosome, protein synthesis, the ribosome as a molecular machine. regulation of protein synthesis.
Structure of Chromatin and epigenetics.
Nucleosemes, Chromatin organization and gene experssion. Chromatin modification and gene expression.
Other levels of regulation. RNA modification; RNA as a general regulator: siRNA, miRNA, ceRNA, lncRNA.
Methods.
DNA electrophoresis, agarose and polyachrilamide, radioactive label. restriction map, southern blotting, footprinting, PCR:
Laboratory.
Analysis of a transposon in the human genome. extraction of DNA from saliva and amplification of the locus PV 92 and study of the polymorfism in the class.
Planned learning activities and teaching methods: frontal lessons and laboratory sessions
Additional notes about suggested reading: An e-learning platform is active where several papers in english are present.
power point presentations are mostly in english.
Textbooks (and optional supplementary readings)

Innovative teaching methods: Teaching and learning strategies
  • Laboratory
  • Working in group
  • Work-integrated learning
  • Use of online videos
  • Loading of files and pages (web pages, Moodle, ...)