First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Science
Course unit
SCP3056653, A.A. 2019/20

Information concerning the students who enrolled in A.Y. 2017/18

Information on the course unit
Degree course First cycle degree in
IF1839, Degree course structure A.Y. 2011/12, A.Y. 2019/20
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Number of ECTS credits allocated 6.0
Type of assessment Mark
Website of the academic structure
Department of reference Department of Biology
Mandatory attendance
Language of instruction Italian
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Teacher in charge GIACOMO SAIELLI

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Educational activities in elective or integrative disciplines CHIM/06 Organic Chemistry 6.0

Course unit organization
Period First semester
Year 3rd Year
Teaching method frontal

Type of hours Credits Teaching
Hours of
Individual study
Laboratory 1.0 16 9.0 No turn
Lecture 5.0 40 85.0 No turn

Start of activities 30/09/2019
End of activities 18/01/2020
Show course schedule 2019/20 Reg.2011 course timetable

Examination board
Board From To Members of the board
6 METODI FISICI IN CHIMICA BIORGANICA 2018-2019 01/10/2018 30/11/2019 SAIELLI GIACOMO (Presidente)
GOBBO MARINA (Membro Effettivo)

Prerequisites: Knowledge and understanding of general organic chemistry and physical chemistry.
Target skills and knowledge: The course will provide students with the means to interpret spectra obtained with the most common techniques used for structural elucidation of molecules: mass spectrometry (MS), applied to small organic molecules and proteins; infrared (IR) spectroscopy, for organic molecules and proteins; nuclear magnetic resonance (NMR), applied to small organic molecules and natural substances.
Examination methods: Written test: multiple choice tests and interpretation of spectra of unknown compounds
Assessment criteria: The student must demonstrate the ability to interpret MS, IR and NMR spectra and to be able to guess the correct molecular structure from the analysis of the spectra.
Course unit contents: Lectures:
Principles of organic chemistry: functional groups, nomenclature, inductive and resonance effects.
General principles about the spectroscopic techniques of structural elucidation.
Mass Spectrometry (MS): Ionization methods: electron impact, chemical ionization, MALDI, ESI. Ion separation methods: magnetic, quadrupolar and time-of-flight analyzers. Molecular ion, isotopic ions, metastable ions. Mass spectrometry to study proteins: peptide fingerprinting, peptide sequence tag.
Infrared spectroscopy (IR): IR resonances of the principal functional groups of organic chemistry and proteins.
Nuclear Magnetic resonance (NMR): chemical shift and shielding constant. Factors influencing the chemical shift: electronic structure and π electrons. Additivity rules. Coupling constant J. Spin multiplicity and its origin. Geminal, vicinal and long-range coupling. Factors influencing the coupling constant. Chemical shift and coupling in hydrogen atoms bonded to O, N and S atoms.
Chemical and magnetic equivalence, enantiotopic and diastereotopic protons. First order and higher order spectra. Dynamic NMR. Couplings with heteroatoms (C-13, F-19, P-31). Decoupling and NOE effect. 
Problem of interpretation of MS, IR and NMR spectra of unknown compounds
Preparation of samples of unknown compounds for MS, IR and NMR spectra. Acquisition and analysis of the spectra. Extraction of natural substances and subsequent identification.
Planned learning activities and teaching methods: Lectures, exercise, laboratory experiments
Additional notes about suggested reading: Lecture notes, downloadable from the lecturer web site, suggested text
Textbooks (and optional supplementary readings)
  • Silverstein, Robert M.; Webster, Francis X.; Pinna, Luigi; Rassu, Gloria; Casiraghi, Giovanni; Zanardi, Franca, Identificazione spettroscopica di composti organiciRobert M. Silverstein, Francis X. Websteredizione italiana a cura di Giovanni Casiraghi, Luigi Pinnahanno collaborato alla traduzione Gloria Rassu, Franca Zanardi. Milano: CEA, 1999. Cerca nel catalogo