First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Science
BIOTECHNOLOGY
Course unit
MOLECULAR VIROLOGY
SCP3056622, A.A. 2019/20

Information concerning the students who enrolled in A.Y. 2017/18

Information on the course unit
Degree course First cycle degree in
BIOTECHNOLOGY
IF1839, Degree course structure A.Y. 2011/12, A.Y. 2019/20
N0
bring this page
with you
Number of ECTS credits allocated 7.0
Type of assessment Mark
Course unit English denomination MOLECULAR VIROLOGY
Website of the academic structure https://biotecnologie.biologia.unipd.it/lt-biotecnologie/
Department of reference Department of Biology
Mandatory attendance
Language of instruction Italian
Branch PADOVA
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge CRISTIANO SALATA MED/07

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Educational activities in elective or integrative disciplines MED/07 Microbiology and Clinical Microbiology 7.0

Course unit organization
Period Second semester
Year 3rd Year
Teaching method frontal

Type of hours Credits Teaching
hours
Hours of
Individual study
Shifts
Lecture 7.0 56 119.0 No turn

Calendar
Start of activities 02/03/2020
End of activities 12/06/2020
Show course schedule 2019/20 Reg.2011 course timetable

Examination board
Board From To Members of the board
8 VIROLOGIA MOLECOLARE 2019-2020 01/10/2019 27/11/2020 SALATA CRISTIANO (Presidente)
DEL VECCHIO CLAUDIA (Membro Effettivo)
CALISTRI ARIANNA (Supplente)
7 VIROLOGIA MOLECOLARE 2018-2019 01/10/2018 30/11/2019 MERCORELLI BEATRICE (Presidente)
CALISTRI ARIANNA (Membro Effettivo)
DEL VECCHIO CLAUDIA (Membro Effettivo)
PROVVEDI ROBERTA (Supplente)

Syllabus
Prerequisites: To follow this course the rerequisites are basic knowledge of Genetics, Molecular Biology, Biochemistry, Immunology and Microbiology. Moreover, also some basic notions of structural biology are appreciated.
Target skills and knowledge: This couse promts the student to acquire deep insights into the molecular biology of the replication cycle of viruses belonging to different families, by analyzing the different steps of virus replication cycle in a general vision.
Moreover, the student will acquire expertise in virus titer determination and measurement of virus particles with different, both cultural and indirect methods. Furthermore, the student will acquire knowledge of virus genomes manipulation for biotecnology applications such as transgene expression, gene editing, gene therapy, oncolytic virotherapy, and vaccine production. Finally, the student will acquire competences concerning the comprehension of the molecular basis of viral pathogenesis and of virus-host interactions, particularly of viral human pathogens.
Examination methods: The final examination will be both written with a test with multiple choice questions; for the oral part, the student will held a brief presentation on a topic chosen by the student itself that will allow the student to gain up to 2 additional points on the final mark.
Assessment criteria: For the final evaluation the following aspects will be taken into account:
1) knowledge of the topics covered in class;
2) insights into the topics and completeness of answers, clarity, scientific language and appropriate capacity of synthesis;
3) ability to link the subjects covered in the course to knowledge previously acquired in related courses.
Course unit contents: Brief introduction on viruses and the history of the study of viruses. General methods to measure virus particles (cultural methods and indirect methods based on biochemical methods and structural biology methods).
Structure of virions: symmetry of capsids and architecture of virions. Viral capsid interactions with the host cell
Genetics of viruses and description of the structures of the viral genomes belonging to the seven classes of viruses.
Mechanisms of viral replication: the viral cycle steps. Comparison between the different classes of viruses.
Expression of genetic information and control: comparison of the strategies developed by the various classes of virus and analysis of some specific mechanisms of expression control.
Introduction to the manipulation of viral genomes to advanced biotechologies: reverse genetics, rescue of viral recombinant genomes, vaccine development, oncolytic virotherapy, use of viral vectors for gene transfer and gene therapy, CAR-T therapy and combined immunotherapy.
The mechanisms of pathogenesis at the molecular level: mechanisms of cell damage and cell transformation by DNA and RNA viruses.
Subviral agents: satellites, viroids, and prions.
Insights on human viruses of clinical interest.
Planned learning activities and teaching methods: During the course, the classic lecture will be alternated to innovative teaching methods that aim to obtaining the active partecipation of the students to the learning process (active learning). This will be achieved by continuatively taking the students to reflection on the topics of the course and by asking questions on previous handled topics. Morevoer, the active learning will be pursued with the use of e-learning technologies that involve the use of smartphones to answer to quiz and to actively participate to the lessons. Moreover, also group activities will be envisaged to favor peer education. During the second part of the course the students will have the oportunity to present some research material produced by themselves that will aid both the review of the acquired knowledge and the polishing of soft skills.
Additional notes about suggested reading: Students will be provided with copies of the slides used in class.
Textbooks (and optional supplementary readings)
  • Flint, S.J., Principles of virology, molecular biology, pathogenesis and control of animal viruses S.J. Flint ... [et al.]. Washington: ASM, 2015. Cerca nel catalogo
  • Prescott, Lansing M.; Willey, Joanne M.; Sherwood, Linda M.; Woolverton, Christopher, Prescott's microbiologyJoanne M. Willey, Linda M. Sherwood, Christopher J. Woolverton. New York: McGraw-Hill, 2011. Cerca nel catalogo
  • Cann, Alan J; Campadelli-Fiume, Gabriella, Elementi di virologia molecolare Alan J. Cann edizione italiana a cura di Gabriella Campadelli-Fiume. Milano: CEA, 2006. Cerca nel catalogo

Innovative teaching methods: Teaching and learning strategies
  • Interactive lecturing
  • Working in group
  • Flipped classroom
  • Peer feedback
  • Auto correcting quizzes or tests for periodic feedback or exams
  • Active quizzes for Concept Verification Tests and class discussions
  • Use of online videos

Innovative teaching methods: Software or applications used
  • Moodle (files, quizzes, workshops, ...)

Sustainable Development Goals (SDGs)
Good Health and Well-Being Quality Education