First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Science
BIOTECHNOLOGY
Course unit
MOLECULAR BIOLOGY
SC04100929, A.A. 2018/19

Information concerning the students who enrolled in A.Y. 2017/18

Information on the course unit
Degree course First cycle degree in
BIOTECHNOLOGY
IF1839, Degree course structure A.Y. 2011/12, A.Y. 2018/19
N0
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Number of ECTS credits allocated 7.0
Type of assessment Mark
Course unit English denomination MOLECULAR BIOLOGY
Website of the academic structure https://biotecnologie.biologia.unipd.it/index.php?id=113
Department of reference Department of Biology
E-Learning website https://elearning.unipd.it/biologia/course/view.php?idnumber=2018-IF1839-000ZZ-2017-SC04100929-N0
Mandatory attendance
Language of instruction Italian
Branch PADOVA
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge ELISABETTA BERGANTINO BIO/11

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Core courses BIO/11 Molecular Biology 7.0

Course unit organization
Period First semester
Year 2nd Year
Teaching method frontal

Type of hours Credits Teaching
hours
Hours of
Individual study
Shifts
Laboratory 1.0 16 9.0 No turn
Lecture 6.0 48 102.0 No turn

Calendar
Start of activities 01/10/2018
End of activities 18/01/2019

Examination board
Board From To Members of the board
7 BIOLOGIA MOLECOLARE 2018-2019 01/10/2018 30/11/2019 BERGANTINO ELISABETTA (Presidente)
FILIPPINI FRANCESCO (Membro Effettivo)
CAMPANARO STEFANO (Supplente)
6 BIOLOGIA MOLECOLARE 2017/2018 01/10/2017 25/11/2018 BERGANTINO ELISABETTA (Presidente)
FILIPPINI FRANCESCO (Membro Effettivo)
CAMPANARO STEFANO (Supplente)

Syllabus
Prerequisites: Biochemistry, Cell Biology, Genetics.
Target skills and knowledge: The students should understand the relationship between organization and function of the molecules such as nucleic acids and protein inside the cell. The course will provide the basic elements to analyze genome expression using molecular genetics tools.
Examination methods: written with open and multiple-choice questions
Assessment criteria: Acquisition of a specific and appropriate language on the field of molecular biology
Understanding the levels of regulation of genome expression in bacterial and eukaryotic cells
Course unit contents: THE ORIGIN OF MOLECULAR BIOLOGY.
The nature of genetic material, the double helix, the central dogma
STRUCTURES OF DNA
DNA A, B, Z, phisycal parameters, local alternative conformations, cruciforms, curved DNA, DNA Topology and Topoisomerases.
STRUCTURES OF RNA
tRNA, rRNA, the genetic code and amber suppressors.
DNA REPLICATION
The replisome, DNA Polymerases,replication fidelity, proofreading activity, replication of circular and linear DNA, telomeres,
Models of replication, origins of replication in bacterial and eukaryotic cells.
RNA TRANSCRIPTION IN BACTERIA
RNA Polymerase, subunits, inhibitors, mutants, closed and open complex, promoters, sigma factor, phage lambda regulation, The operon, lac operon, trp operon.
TRANSCRIPTION IN EUKARYOTIC CELLS
RNA Polimerases I, II, III. Promoters, enhancers, general transcripion factors, the mediator, transcriptional activators. the combinatorial control of gene expression.
RNA PROCESSING
Spicing, alternative splicing, cathalytic RNA. RNA world, regulation of gene expression by RNA.
TRANSLATION
RNA of the ribosome, protein synthesis, the ribosome as a molecular machine. regulation of protein synthesis.
STRUCTURE OF CHROMATIN and EPIGENETICS
Nucleosemes, Chromatin organization and gene experssion. Chromatin modification and gene expression.
OTHER LEVELS OF REGULATION
RNA modification; RNA as a general regulator: siRNA, miRNA, ceRNA, lncRNA.
METHODS
DNA electrophoresis, agarose and polyachrilamide, radioactive label. restriction map, southern blotting, footprinting, PCR.
Planned learning activities and teaching methods: Classroom lectures and laboratory sessions.
Additional notes about suggested reading: Reference textbooks.
An e-learning platform is active where students, after subscription, can be part of a forum where questions and requests of clarification are answered. Power point presentations and some relevant scientific papers are presented.
Textbooks (and optional supplementary readings)
  • Amaldi, Francesco, Benedetti Piero, Pesole Graziano, Plevani Paolo, Biologia molecolare. Rozzano (MI): Casa Editrice Ambrosiana, 2017. 3 a edizione Cerca nel catalogo
  • Watson JD, Biologia molecolare del gene. Milano: Zanichelli, 2015. 7 a edizione Cerca nel catalogo

Innovative teaching methods: Teaching and learning strategies
  • Laboratory
  • Working in group
  • Questioning
  • Peer feedback
  • Peer assessment
  • Active quizzes for Concept Verification Tests and class discussions

Innovative teaching methods: Software or applications used
  • Moodle (files, quizzes, workshops, ...)
  • Kahoot / Poll Everywhere

Sustainable Development Goals (SDGs)
Good Health and Well-Being Quality Education Decent Work and Economic Growth Responsible Consumption and Production