First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Agricultural Sciences and Veterinary Medicine
Course unit
AVP7077602, A.A. 2019/20

Information concerning the students who enrolled in A.Y. 2017/18

Information on the course unit
Degree course First cycle degree in
AV2378, Degree course structure A.Y. 2017/18, A.Y. 2019/20
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Degree course track LAB ANIMAL [001LE]
Number of ECTS credits allocated 10.0
Type of assessment Mark
Website of the academic structure
Department of reference Department of Comparative Biomedicine and Food Science
E-Learning website
Mandatory attendance
Language of instruction English
Single Course unit The Course unit CANNOT be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Other lecturers BARBARA CAROBBI VET/09

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Educational activities in elective or integrative disciplines VET/07 Veterinary Pharmacology and Toxicology 2.0
Core courses VET/09 Clinical Veterinary Surgery 8.0

Course unit organization
Period First semester
Year 3rd Year
Teaching method frontal

Type of hours Credits Teaching
Hours of
Individual study
Practice 3.0 24 51.0 No turn
Lecture 7.0 56 119.0 No turn

Start of activities 30/09/2019
End of activities 18/01/2020
Show course schedule 2019/20 Reg.2017 course timetable

Examination board
Board From To Members of the board
1  Commissione a.a. 2019/20 01/12/2019 30/11/2020 DE BENEDICTIS GIULIA MARIA (Presidente)
CAROBBI BARBARA (Membro Effettivo)
GIANTIN MERY (Supplente)

Prerequisites: Students are required to have basic knowledge of pharmaco-toxicology, physiological basis of pain, suffering and distress and general ethology, animal husbandry and welfare.
Required foundation courses: Applied mathematics and physics, General pathology and laboratory techniques. Admission of students to practical activities (which is mandatory for at least 75% of the laboratory hours) is conditional depending on compliance of appropriate educational background.
Target skills and knowledge: The course aims to adequately educate and train the student to take care of animals involved in scientific research. The student will be prepared to the following skills, according to the principles of 3R: recognizing and reducing animal suffering; carrying out minor procedures; understanding the experimental protocol; taking care of the animal during the experimental period; understanding the humane endpoint; acquiring the different methods used for euthanasia.
Examination methods: The oral comprehensive exam will consist in an interview based on questions referring to the course syllabus.
Assessment criteria: The criteria for student evaluation include the degree of knowledge understanding and assimilation of the topics covered in class, the capability of making intra- and inter-disciplinary connections, and the use of appropriate technical language.
Course unit contents: General part:
Animal care, health and management in clinical settings. Methods for marking individual animals.
Basic concept of laboratory animal health status assessment and introduction to diseases of laboratory animals.
Humane animal handling and restraint. Clinical examination and welfare assessment. Drugs and fluids administration. Blood sampling (blood volume, techniques, equipment preparation, anatomical landmarks).
Elements of biomedical imaging (X-Ray, ultrasound, MRI, CT, PET).
Concepts of laboratory animals’ anaesthesia and emergencies.
Anaesthesia for minor procedures. Anaesthesia for prolonged procedures (grater than 15 minutes).
Record-keeping: data sheet, score sheet design, observation sheet, clinical score, severity assessment sheet, practical use of score sheet.
Severity of procedures, severity classification system and action to take.
Humane endpoints and humane methods of euthanasia.
Notes of occupational health and safety when working with laboratory animals.

The course will focus on basic surgical principles and procedures.
Principles of surgical asepsis; sterilization of surgical equipment; surgical facilities and equipment; care and maintenance of the surgical environment; preparation of the surgical patient and the operating team; surgical instruments; suture materials; removal of suture or wound clips. Basic operative techniques. Principles of wound and tissue healing. Common post-surgical complications and their causes. Planning of surgical procedures. Principles of post-surgical care, nursing and monitoring. Examples of surgical models.

Pain assessment:
Minimizing pain and distress in clinical settings. Specie specific recognition and management of pain, suffering and distress : laboratory rodents, rabbit, small ruminants, pigs and other animals used in scientific research. Clinical application of the pain scales. Pain management in laboratory animals. Practical assessment of pain/suffering in lab animals (by the use of pictures, videos and interactive activities).

In vivo preclinical studies and in vitro alternative methods:
General concepts on in vivo methodologies used for pharmacokinetic and pharmacodynamics studies; drug discovery and development; preclinical and clinical investigations, particularly referring to acute and chronic toxicity studies, mutagenesis, carcinogenesis, and reproductive toxicology; basic concepts on alternative in vitro methods and their use in pharmaco-toxicological investigations.

Practical activities:
- demonstration of procedures/techniques and interactive discussion of clinical cases, in-class session of pain assessment in different species, meeting with experts in laboratory animal medicine. Practical skills will be developed on simulators and cadavers (8 hours);
- lab session on surgical instruments, basic surgical techniques, and preparation of the surgical patient and the surgical team (8 hours);
- genotyping nuclear receptors (nucleic acid extraction from blood/tissue; execution qPCR assay; result evaluation and phenotypic consequences); draft of a pharmaco-toxicological experimental protocol; critical reading of an article focusing on experimental pharmaco-toxicology (8 hours).
Planned learning activities and teaching methods: The course includes 56 hours of lectures and 24 hour of practicals and hands on teaching, to strengthen concepts and principles discussed in lecture sessions.
Practical activities include:
- demonstration of procedures/techniques
- interactive discussion of clinical cases
- practicals on simulators and cadavers
- laboratory activities
- draft of experimental protocols
- critical reading of scientific papers
Additional notes about suggested reading: - Karen Hrapkiewicz, Lesley Colby, Patricia Denison. Clinical laboratory animal medicine (4th edition) 2013 Wiley Blackwell, ISBN: 978-1-118-34510-8
- Victoria Aspinall: Clinical Procedures in Veterinary Nursing (3rd edition) 2014; Elsevier, ISBN: 9780702051104
- Elizabeth Mullineaux, Marie Jones (ed): BSAVA Manual of Practical Veterinary; Wiley, ISBN: 978-0-905214-91-7
- Dennis F. Kohn, Sally K. Wixson, William J. White and G. John Benson (ed): Anesthesia and Analgesia in Laboratory Animals; Elsevier, ISBN: 978-0-12-417570-9

Microsoft Power Point slide presentations (PDF format) will be available on Moodle platform (
Textbooks (and optional supplementary readings)
  • Karen Hrapkiewicz, Lesley Colby, Patricia Denison., Clinical laboratory animal medicine. --: Wiley Blackwell, 2013. ISBN: 978-1-118-34510-8 Cerca nel catalogo
  • Victoria Aspinall, Clinical Procedures in Veterinary Nursing. --: Elsevier, 2014. ISBN: 9780702051104 Cerca nel catalogo
  • Dennis F. Kohn, Sally K. Wixson, William J., Anesthesia and Analgesia in Laboratory Animals. --: Elsevier, 2008. ISBN: 978-0-12-417570-9 Cerca nel catalogo

Innovative teaching methods: Teaching and learning strategies
  • Lecturing
  • Laboratory
  • Problem based learning
  • Case study
  • Interactive lecturing
  • Problem solving
  • Use of online videos
  • Loading of files and pages (web pages, Moodle, ...)
  • Learning journal

Innovative teaching methods: Software or applications used
  • Moodle (files, quizzes, workshops, ...)

Sustainable Development Goals (SDGs)
Quality Education Life Below Water Life on Land