First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Agricultural Sciences and Veterinary Medicine
Course unit
AVP7077579, A.A. 2019/20

Information concerning the students who enrolled in A.Y. 2017/18

Information on the course unit
Degree course First cycle degree in
AV2378, Degree course structure A.Y. 2017/18, A.Y. 2019/20
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Degree course track LAB ANIMAL [001LE]
Number of ECTS credits allocated 8.0
Type of assessment Mark
Course unit English denomination COMPARATIVE PATHOLOGY
Website of the academic structure
Department of reference Department of Comparative Biomedicine and Food Science
E-Learning website
Mandatory attendance
Language of instruction English
Single Course unit The Course unit CANNOT be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Teacher in charge RANIERI VERIN VET/03
Other lecturers LUCA BARGELLONI BIO/13

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Educational activities in elective or integrative disciplines BIO/13 Applied Biology 2.0
Core courses VET/03 General Pathology and Veterinary Pathological Anatomy 6.0

Course unit organization
Period First semester
Year 3rd Year
Teaching method frontal

Type of hours Credits Teaching
Hours of
Individual study
Laboratory 3.0 24 51.0 No turn
Lecture 5.0 40 85.0 No turn

Start of activities 30/09/2019
End of activities 18/01/2020
Show course schedule 2019/20 Reg.2017 course timetable

Examination board
Board From To Members of the board
1  Commissione a.a. 2019/20 01/12/2019 30/11/2020 VERIN RANIERI (Presidente)
BARGELLONI LUCA (Membro Effettivo)

Prerequisites: Students are required to have solid knowledge of Comparative Animal Anatomy and Physiology, General pathology and principles of Infectious and Parasitic diseases.
Required foundation courses: Applied mathematics and physics, General pathology and laboratory techniques. Admission of students to practical activities (which is mandatory for at least 75% of the laboratory hours) is conditional depending on compliance of appropriate educational background.
Target skills and knowledge: The aims of the course are:
-to briefly describe the main animal models used for research purposes and how to get them (spontaneous vs induced models, transgenic models)
to outline:
-procedures for attending and performing a necropsy in laboratory animal species. Specific theoretical knowledge and practical skills will be provided;
-effective collection of specimens performed avoiding the occurrence of artifacts or post mortem change;
-appropriate collection of samples for the intended purpose (i.e. histology, cytology, microbiology, virology, cell cultures, banked tissue, RNA and DNA samples) and adequate in number and amount to provide valid results
- basic knowledge on the main genetic manipulations (knock-out by homologous recombination, knock-down by RNAi, genome editing) with special focus on mouse, rat, and zebrafish
Examination methods: In class tests: active participation during necropsies sessions and microscope sessions with in class tests (30% of the final score).
Final exam (70% of the final score) will cover both theoretical and practical components of the whole course with written open questions and/or multiple choice questions, possibly including post mortem procedure.
Assessment criteria: The examination will cover both practical and theoretical learning objectives. The student is expected to know the post mortem techniques in laboratory animals and to adequately perform the collection procedures.
Assessment criteria include the degree of knowledge of acquired information and the capability of performing the more appropriate procedure and tissue collection.
Course unit contents: The course lectures cover:
-the specific terminology, the aims, the procedures and the instruments for performing necropsies in main animal models. Special efforts will be addressed in data collection and anamnesis.
- Overview ad revision of main anatomical and physiological features of principal lab animal species (mouse, rat, zebrafish, avian species etc) in regard to specific pathological processes.
- the understanding of the main gross and microscopic post mortem changes and the distinguishing features of main pathological processes in principal lab animal species including common background lesions.
- death confirmation and how cadavers should be processed or otherwise disposed with specific legislation for carcasses disposal (i.e. 1069/09 e 142/11)

-techniques and procedures involved in sample collection, submission, labeling and storage. Samples may be taken from animals and/or environment for diagnostic purposes, disease surveillance, health certification, or monitoring the response to a specific treatment or research, avoiding the occurrence of artifacts or post mortem change in the collected tissues. Minor techniques for tissue sampling, appropriate sample volumes and sampling frequencies for the relevant species will be taught. Rigour and consistency for correct recording and handling of samples.

-the safety procedures of collecting samples to avoid danger to the operator or tissue damage.
-the sampling for cytology and histology: collection procedures, staining methods, fixation.
The course lab sessions and practical activities cover:
-virtual lab to understand the main post mortem techniques. Necropsies will be performed in post mortem room on laboratory animal species (mouse, rat, fish, avian, pigs etc)
-Cytology smears will be performed on carcasses, stained and observed on light microscopy.
-Histology will be performed by sampling animals of post mortem room. Samples will be processed in anatomic pathology lab and observed on light microscopy.
-spontaneous and induced animal models
-animal cloning through somatic nuclear gene transfer
-how to obtain transgenic animals with special attention to mouse, rat, and zebrafish
-gene targeting via homologous recombination: Gene knock-out, knock-in, conditional models
-genome editing
Planned learning activities and teaching methods: The course includes 40 hours of lectures and 24 hours of lab sessions. Lectures will be performed with ppt slides projection Seminars and workshops with practitioners and pathologists of laboratory animals will be organized.
Lab sessions and practical activities on animals and tissues will be performed in post mortem room, pathology lab, microscopy room. Active learning will be implemented using online quiz to check student progress and stimulate discussion in the classroom. Topics will be analysed using a problem-solving approach trhough the evaluation of case studies and practical examples.
Additional notes about suggested reading: Detailed learning objectives and pdf slides and schedule are available on Moodle (
Textbooks (and optional supplementary readings)
  • L.A Anderson, G. Otto, K. R. Pritchett-Corning, M.T. Whary and J. G. Fox, Laboratory Animal Medicine. --: --, --. ISBN: 978-0-12-409527-4 Cerca nel catalogo
  • James G. Fox, Lynn C. Anderson, Franklin M. Loew and Fred W. Quimby, Laboratory Animal Medicine (2nd Edition) a volume in American colleg of laboratory animal medicine. --: --, --. ISBN: 978-0-12-263951-7 Cerca nel catalogo