First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Agricultural Sciences and Veterinary Medicine
BIOTECHNOLOGIES FOR FOOD SCIENCE
Course unit
TOXICOGENOMICS AND TOXICOLOGIC PATHOLOGY
AVP6075240, A.A. 2018/19

Information concerning the students who enrolled in A.Y. 2017/18

Information on the course unit
Degree course Second cycle degree in
BIOTECHNOLOGIES FOR FOOD SCIENCE
IF0362, Degree course structure A.Y. 2017/18, A.Y. 2018/19
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Degree course track BIOTECNOLOGIE PER L'ALIMENTAZIONE [001LE]
Number of ECTS credits allocated 12.0
Type of assessment Mark
Course unit English denomination TOXICOGENOMICS AND TOXICOLOGIC PATHOLOGY
Department of reference Department of Comparative Biomedicine and Food Science
Mandatory attendance No
Language of instruction Italian
Branch LEGNARO (PD)
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge MAURO DACASTO VET/07
Other lecturers MARIA ELENA GELAIN VET/03
MERY GIANTIN VET/07

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Core courses VET/03 General Pathology and Veterinary Pathological Anatomy 3.0
Core courses VET/07 Veterinary Pharmacology and Toxicology 6.0
Core courses MED/43 Legal Medicine 3.0

Course unit organization
Period First semester
Year 2nd Year
Teaching method frontal

Type of hours Credits Teaching
hours
Hours of
Individual study
Shifts
Practice 2.0 16 34.0 2
Lecture 10.0 80 170.0 No turn

Calendar
Start of activities 01/10/2018
End of activities 18/01/2019
Show course schedule 2018/19 Reg.2017 course timetable

Examination board
Board From To Members of the board
2  Commissione a.a. 2018/19 01/12/2018 30/11/2019 DACASTO MAURO (Presidente)
GELAIN MARIA ELENA (Membro Effettivo)
GIANTIN MERY (Supplente)
1  Commissione a.a. 2017/18 01/12/2017 30/11/2018 GIANTIN MERY (Presidente)
DACASTO MAURO (Membro Effettivo)
GELAIN MARIA ELENA (Supplente)

Syllabus
Prerequisites: There are no specific requirements. Helpful the knowledge about form, structure and functions of simplified or complex living organisms and related bio-molecular mechanisms
Required foundation courses: none
Target skills and knowledge: Knowledge about toxicity (including toxicokinetics and toxicodynamics) of foremost classes of xenobiotics, either of natural or anthropogenic origin, likely found in foodstuffs to be used in animals and humans.

Knowledge about foremost pharmacogenetic and toxicogenomic general principles and methodologies and specific toxicologic biomarkers; basic information about in vitro methods (i.e., cell cultures) used in pharmaco-toxicological bio-molecular studies as well as analytical techniques used for the detection of residues in food from animal producing species.

Principles of methods validation and method comparison; immunological techniques and their application; knowledge and interpretation of clinico-pathological alterations due to toxic injury against the main systems and/or organs.
Examination methods: Oral exam
The examination will be performed the same day for the 3 modules.
For each module, two open questions and one short question will be asked.
In details, open questions will concern:
- a molecular mechanism of toxicity; a class of toxic compounds;
- in vitro toxicology; toxicogenomics/pharmacogenetics;
- analitical methods; tissue-specific biomarkers.
Assessment criteria: Evaluation of the acquired knowledge about specific arguments; the student’s capability to discuss and solve specific problems and make by itself intra- and inter-disciplinary connections; the use of a specific scientific language
Course unit contents: FOOD TOXICOLOGY (3 UFC)
Fundamental toxicology: toxicokinetics, toxicodynamics, bioactivation, dose-response curve, acute, subacute and chronic toxicity, chemical carcinogenesis, mutagenesis, reproductive toxicology.
Fundamental molecular toxicology: oxidative stress, altered calcium homeostasis, impairment of cell proliferation, covalent binding, enzymes and other proteins inactivation, interaction with ion transporters, nuclear receptor-mediated toxicity, altered cellular signal transduction, mitochondrial toxicity
Toxic phytochemicals: favism, goitrogens, cyanogenetic glycosides, phytoalexin.
Toxin from fungi: mycotoxins, amanita phalloides
Natural toxins in marine foodstuffs
Anthropogenic contaminants: plychlorinated biphenyls (PCBs), polychlorinated dibenzo-dioxins (PCDDs), heavy metals.
Pesticides: insecticides, herbicides.
Toxicants formed during food processing: polycyclic aromatic hydrocarbons (PAHs), polycyclic aromatic amines (PAAs), N-nitrosamines, acrylamide.

PHARMACOGENETICS, TOXICOGENOMICS AND IN VITRO TOXICOLOGY (6 UFC)
Basic knowledge about in vitro methods; the cellular biology laboratory, medium and reagents; cell cultures and validation of alternative in vitro methods. Cytotoxicity studies. In vitro methods for the study of metabolism. In vitro studies on expression and regulation of drug metabolizing enzymes. Use of transfection, infection and gene silencing in toxicology.
Biomarkers, definition, classification and examples. Pharmacogenetics: principles and techniques. Toxicogenomics: principles and techniques. Surrogate tissue analysis. Examples of the application of recent methodologies in residues toxicology, environmental toxicology, food toxicology.
Lab sessions: spectrophotometric determination of acetyilcholinesterase activity in bovine serum; cells thawing and seeding; medium changing, trypsinization and freezing; incubation with xenobiotics and cytotoxicity testing; enzyme immunoassay (ELISA kit) for the detection of algal toxins.

TOXICOLOGIC PATHOLOGY (3 UFC)
In vivo methods: principles of method validation and method comparison. Principles of immunological techniques. Biomarkers of tissue-specific toxic damages: main clinic-pathological alterations in renal and hepatic injury and in damages against hematopoietic and immune, central nervous and cardiovascular system. Case discussion.
Planned learning activities and teaching methods: Altogether, the course includes:
80 hours of frontal teaching
16 hours of practicals and hands on teaching
1 round of supplementary practicals and hands on teaching
Additional notes about suggested reading: Microsoft Power Point slide presentations are available on Moodle platform (https://elearning.unipd.it/scuolaamv/).

Consulting hour: by appointment with the lecturer
Textbooks (and optional supplementary readings)
  • Shibamoto T.& Bjeldanes L.F. (Eds.), Introduction to Food Toxicology, 2nd Edition. --: Academic Press (Elsevier), 2009. ISBN: 978-0-12-374286-5 Cerca nel catalogo
  • Dolara, P., TOX – Lezioni di Tossicologia. --: Firenze University Press, 2006. ISBN 88-8453-412-7 Cerca nel catalogo
  • Boelsterli U.A. (Ed.), Mechanistic toxicology, 2nd edition. --: Taylor & Francis, 2009. ISBN: 0-8493-7272-0
  • G.O. Evans, Animal Clinical Chemistry: A Practical Handbook for Toxicologists and Biomedical Researchers. --: CRC Press, --. ISBN 978-1420080117
  • A. Wallace Hayes, Claire L. Kruger, Hayes' Principles and Methods of Toxicology. --: CRC Press, --. ISBN 9781842145364

Innovative teaching methods: Teaching and learning strategies
  • Lecturing
  • Laboratory
  • Case study
  • Working in group
  • Peer assessment
  • Active quizzes for Concept Verification Tests and class discussions
  • Loading of files and pages (web pages, Moodle, ...)
  • Learning journal

Innovative teaching methods: Software or applications used
  • Moodle (files, quizzes, workshops, ...)
  • Kahoot

Sustainable Development Goals (SDGs)
Good Health and Well-Being