First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Medicine
PHARMACY
Course unit
MOLECULAR BIOLOGY
FAO2043752, A.A. 2018/19

Information concerning the students who enrolled in A.Y. 2017/18

Information on the course unit
Degree course 5 years single cycle degree in
PHARMACY (Ord. 2009)
FA1732, Degree course structure A.Y. 2009/10, A.Y. 2018/19
N0
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Number of ECTS credits allocated 6.0
Type of assessment Mark
Course unit English denomination MOLECULAR BIOLOGY
Department of reference Department of Pharmaceutical and Pharmacological Sciences
E-Learning website https://elearning.unipd.it/dsf/course/view.php?idnumber=2018-FA1732-000ZZ-2017-FAO2043752-N0
Mandatory attendance No
Language of instruction Italian
Branch PADOVA
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge GEPPO SARTORI BIO/11

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Core courses BIO/11 Molecular Biology 6.0

Course unit organization
Period Second semester
Year 2nd Year
Teaching method frontal

Type of hours Credits Teaching
hours
Hours of
Individual study
Shifts
Lecture 6.0 48 102.0 No turn

Calendar
Start of activities 25/02/2019
End of activities 14/06/2019
Show course schedule 2019/20 Reg.2018 course timetable

Examination board
Board From To Members of the board
6 Commissione a.a. 2018/19 03/12/2018 30/09/2019 SARTORI GEPPO (Presidente)
SANDONA' DORIANNA (Membro Effettivo)

Syllabus
Prerequisites: Basic knowledge on prokariotic and eukariotic cell structure. Basic biochemistry concepts, mostly concerning protein and nucleic acid structure.
Target skills and knowledge: The student will learn the molecular mechanisms of some basic cellular functions, like DNA replication, transcription and translation along with some important regulatory mechanism of these mechanisms. For some selected topics, the student will appreciate the important differences, but also the similarities, that characterize these processes in prokaryotes and eukaryotes. Moreover, the student will acquire a deeper knowledge of certain signal pathways, involved in the onset of serious diseases. Finally, the student will acquire a basic knowledge of the main techniques allowing in vitro and in vivo DNA manipulation, as well as the genome, transcriptome and proteome analysis.
Examination methods: The final exam will consist in a written test with both open-ended and around 30 multiple-choice questions.
Assessment criteria: The evaluation of the student will take into account not only the knowledge in the field of the main thematic areas addressed, but also the capacity to evaluate and solve various problems related to the study of specifics molecular mechanisms. For this puprose, in particular, the student will be asked to correctly analyze and interpret the results of one or more standard experiments.
Course unit contents: Introduction to the macromolecules: DNA, RNA and protein structure, examples of macromolecule interactions and structure-function correlations.
DNA replication: the DNA replication chemistry, DNA polymerase mechanism of action, replicative fork, replication start and ending.
DNA transcription: transcription in prokaryotes, transcription regulation in prokaryotes (lac and trp operons), transcription in eukaryotes (initiation-termination), mRNA maturation, some examples of transcription regulation in eukaryotes, miRNAs and interfering RNAs.
Translation: mRNA, tRNA, ribosomes, translation factors, amynoacyl-tRNA-synthetases.
Recombinant DNA technology: bacterial enzymes used as in vitro tools, cloning vectors and strategies, PCR and its applications, mutagenesis and strategies in recombinant protein expression, new fronteers in the “omic” technologies.
Planned learning activities and teaching methods: The course is organized in lectures. During the lectures students will be periodically involved and stimulated to actively join discussions about in depth study of specific topics.
Additional notes about suggested reading: Slides and other materials used during the course will be available directly on the PC present in the classroom and online on the Moodle platform.
Textbooks (and optional supplementary readings)
  • Robert F. Weaver, Biologia Molecolare. --: McGraw-Hill, 2009. Ottimo testo, sia dal punto di vista introduttivo sia per diversi specifici approfondimenti. Da utilizzare come testo di studio Cerca nel catalogo
  • Lewin B. et al., Il gene X. --: Zanichelli, 2012. Consigliato per approfondimenti Cerca nel catalogo

Innovative teaching methods: Teaching and learning strategies
  • Problem based learning
  • Questioning
  • Problem solving
  • Use of online videos

Innovative teaching methods: Software or applications used
  • Moodle (files, quizzes, workshops, ...)

Sustainable Development Goals (SDGs)
Quality Education