First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Science
BIOTECHNOLOGY
Course unit
PHYSICAL METHODS IN BIOORGANIC CHEMISTRY
SCP3056653, A.A. 2018/19

Information concerning the students who enrolled in A.Y. 2016/17

Information on the course unit
Degree course First cycle degree in
BIOTECHNOLOGY
IF1839, Degree course structure A.Y. 2011/12, A.Y. 2018/19
N0
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Number of ECTS credits allocated 6.0
Type of assessment Mark
Course unit English denomination PHYSICAL METHODS IN BIOORGANIC CHEMISTRY
Website of the academic structure https://biotecnologie.biologia.unipd.it/index.php?id=113
Department of reference Department of Biology
Mandatory attendance
Language of instruction Italian
Branch PADOVA
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge GIACOMO SAIELLI

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Educational activities in elective or integrative disciplines CHIM/06 Organic Chemistry 6.0

Course unit organization
Period First semester
Year 3rd Year
Teaching method frontal

Type of hours Credits Teaching
hours
Hours of
Individual study
Shifts
Laboratory 1.0 16 9.0 No turn
Lecture 5.0 40 85.0 No turn

Calendar
Start of activities 01/10/2018
End of activities 18/01/2019
Show course schedule 2019/20 Reg.2011 course timetable

Examination board
Board From To Members of the board
6 METODI FISICI IN CHIMICA BIORGANICA 2018-2019 01/10/2018 30/11/2019 SAIELLI GIACOMO (Presidente)
GOBBO MARINA (Membro Effettivo)
ZOLEO ALFONSO (Supplente)
5 METODI FISICI IN CHIMICA BIORGANICA 2017/2018 01/10/2017 25/11/2018 SAIELLI GIACOMO (Presidente)
GOBBO MARINA (Membro Effettivo)
ZOLEO ALFONSO (Supplente)

Syllabus
Prerequisites: Knowledge and understanding of general organic chemistry and physical chemistry.
Target skills and knowledge: The course will provide students with the means to interpret spectra obtained with the most common techniques used for structural elucidation of molecules: mass spectrometry (MS), applied to small organic molecules and proteins; infrared (IR) spectroscopy, for organic molecules and proteins; nuclear magnetic resonance (NMR), applied to small organic molecules and natural substances.
Examination methods: Written exam: multiple choice tests and exercises of spectral interpretation
Assessment criteria: The student must demonstrate the ability to interpret MS, IR and NMR spectra and to be able to guess the correct molecular structure from the analysis of the spectra.
Course unit contents: Lectures:
Principles of organic chemistry: functional groups, nomenclature, inductive and resonance effects.
General principles about the spectroscopic techniques of structural elucidation.
Mass Spectrometry (MS): Ionization methods: electron impact, chemical ionization, MALDI, ESI. Ion separation methods: magnetic, quadrupolar and time-of-flight analyzers. Molecular ion, isotopic ions, metastable ions. Mass spectrometry to study proteins: peptide fingerprinting, peptide sequence tag.
Infrared spectroscopy (IR): IR resonances of the principal functional groups of organic chemistry and proteins.
Nuclear Magnetic resonance (NMR): chemical shift and shielding constant. Factors influencing the chemical shift: electronic structure and π electrons. Additivity rules. Coupling constant J. Spin multiplicity and its origin. Geminal, vicinal and long-range coupling. Factors influencing the coupling constant. Chemical shift and coupling in hydrogen atoms bonded to O, N and S atoms.
Chemical and magnetic equivalence, enantiotopic and diastereotopic protons. First order and higher order spectra. Dynamic NMR. Couplings with heteroatoms (C-13, F-19, P-31). Decoupling and NOE effect. 
Exercise:
Problem of interpretation of MS, IR and NMR spectra of unknown compounds
Laboratory:
Preparation of samples of unknown compounds for MS, IR and NMR spectra. Acquisition and analysis of the spectra. Extraction of natural substances and subsequent identification.
Planned learning activities and teaching methods: Lectures, exercise, laboratory experiments
Additional notes about suggested reading: Lecture notes, downloadable from the lecturer web site, suggested text
Textbooks (and optional supplementary readings)
  • Silverstein, Robert M.; Webster, Francis X.; Pinna, Luigi; Rassu, Gloria; Casiraghi, Giovanni; Zanardi, Franca, Identificazione spettroscopica di composti organiciRobert M. Silverstein, Francis X. Websteredizione italiana a cura di Giovanni Casiraghi, Luigi Pinnahanno collaborato alla traduzione Gloria Rassu, Franca Zanardi. Milano: CEA, 2006. Cerca nel catalogo

Innovative teaching methods: Teaching and learning strategies
  • Laboratory
  • Problem solving

Innovative teaching methods: Software or applications used
  • Moodle (files, quizzes, workshops, ...)

Sustainable Development Goals (SDGs)
Good Health and Well-Being Clean Water and Sanitation