First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Science
INDUSTRIAL BIOTECHNOLOGY
Course unit
ADVANCED RECOMBINANT TECHNOLOGIES
SCN1037599, A.A. 2016/17

Information concerning the students who enrolled in A.Y. 2016/17

Information on the course unit
Degree course Second cycle degree in
INDUSTRIAL BIOTECHNOLOGY
SC1731, Degree course structure A.Y. 2014/15, A.Y. 2016/17
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Number of ECTS credits allocated 6.0
Type of assessment Mark
Course unit English denomination ADVANCED RECOMBINANT TECHNOLOGIES
Website of the academic structure http://biotecnologie.scienze.unipd.it/2016/laurea_magistrale
Department of reference Department of Biology
E-Learning website https://elearning.unipd.it/biologia/course/view.php?idnumber=2016-SC1731-000ZZ-2016-SCN1037599-N0
Mandatory attendance
Language of instruction Italian
Branch PADOVA
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge ELISABETTA BERGANTINO BIO/11
Other lecturers MARINA DE BERNARD MED/04

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Core courses BIO/11 Molecular Biology 3.0
Core courses MED/04 General Pathology 3.0

Course unit organization
Period Second semester
Year 1st Year
Teaching method frontal

Type of hours Credits Teaching
hours
Hours of
Individual study
Shifts
Lecture 6.0 48 102.0 No turn

Calendar
Start of activities 27/02/2017
End of activities 09/06/2017

Examination board
Board From To Members of the board
5 TECNOLOGIE RICOMBINANTI AVANZATE 2016-2017 01/10/2016 30/11/2017 BERGANTINO ELISABETTA (Presidente)
DE BERNARD MARINA (Membro Effettivo)
FILIPPINI FRANCESCO (Supplente)

Syllabus
Prerequisites: Molecular Biology (1 and 2, or Molecular Biology of prokaryotic and eukaryotic organisms); principles of Genetic Engineering.
Target skills and knowledge: Subjects of the course are principles and techniques of genetic manipulation of organisms, with particular regards to the expression of recombinant proteins in both well-established and innovative eukaryotic systems. Different host systems will be described, as used in the lab-scale but also in their extension to the industrial and medical fields.
Principles, techniques of production and applications of antibodies and antibody fragments.
Examination methods: Written tests with open questions.
Assessment criteria: Final mark will be the the average result from evaluation of each answer given in the text (the exam will be passed only in the case that both parts of the contents will result equal or higher than 18).
Course unit contents: First part (Recombinant technologies)
Protein expression in E.coli: analysis, design and modification of factors affecting expression of cloned genes.
Protein expression in yeast: Saccharomyces cerevisiae vs Pichia pastoris, similarities and peculiarities. The problem of protein glycosylation.
Protein expression in insect cells: elements of the molecular biology of baculovirus, engineering of its genome; bacmids; ‘humanized’ insect cells.
Protein expression in higher eukaryotic cells. (i) Animal cells: principles of experimental design and production; choice of the cell line and between transient or stable expression. Description of plasmid, viral and retroviral vectors. Engineering of the host cell. (ii) Algal cells: protein expression in Chlamydomonas rehinardtii, the ‘green yeast’.
Trangenic animals as bioreactors: transgenesis in sheep and outlines of transgenesis in other livestock species.
Transgenic plants as bioreactors: elements of molecular biology of useful plant viruses; trangenesis in the chloroplast genome.
Protein synthesis in cell-free systems: applications in the production of membrane proteins and in proteomics. Protein engineering.
For each described expression system, following aspects will be examined: different methods in industrial production and possible analytical, diagnostic and medical applications; paradigmatic applications, both approved for the market and innovative ones, the latter being taken from recent scientific literature.

Second part (Recombinant antibodies)
Production of monoclonal, chimeric, iperchimeric and human antibodies. Applications.
Production of antibody fragments.
Application of phage-display technique to the selection of antibody fragments.
Design of antibodies for therapeutic use (antibodies conjugated to toxins or anti-tumoral drugs).
Planned learning activities and teaching methods: Classroom lectures.
Additional notes about suggested reading: Contents of lessons, scientific articles and reviews will be accessible on the web at the address:
http://elearning.scienze.unipd.it.
Textbooks (and optional supplementary readings)
  • Glick, Pasternak, Patten, Molecular Biotecnology – principles and applications of recombinant DNA - 4th edition. --: ASM press, --. Cerca nel catalogo