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degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Medicine
MEDICINE AND SURGERY
Course unit
ELECTIVE DIDACTIC ACTIVITY IN CLINICAL ANATOMY
MEP4069738, A.A. 2015/16

Information concerning the students who enrolled in A.Y. 2015/16

Information on the course unit
Degree course 6 years single cycle degree in
MEDICINE AND SURGERY (Ord. 2015)
ME1726, Degree course structure A.Y. 2015/16, A.Y. 2015/16
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Number of ECTS credits allocated 4.0
Type of assessment Mark
Course unit English denomination ELECTIVE DIDACTIC ACTIVITY IN CLINICAL ANATOMY
Department of reference Department of Medicine
Mandatory attendance
Language of instruction Italian
Branch PADOVA
Single Course unit The Course unit CANNOT be attended under the option Single Course unit attendance
Optional Course unit The Course unit is available ONLY for students enrolled in MEDICINE AND SURGERY (Ord. 2015)

Lecturers
Teacher in charge ANDREA PORZIONATO BIO/16

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Educational activities in elective or integrative disciplines BIO/16 Human Anatomy 4.0

Course unit organization
Period First semester
Year 1st Year
Teaching method frontal

Type of hours Credits Teaching
hours
Hours of
Individual study
Shifts
Lecture 4.0 32 68.0 No turn

Calendar
Start of activities 19/10/2015
End of activities 29/01/2016
Show course schedule 2019/20 Reg.2015 course timetable

Examination board
Board From To Members of the board
4 COMMISSIONE D'ESAME DI ATTVITIA' DIDATTICA ELETTIVA DI ANATOMIA CLINICA A.A. 2018/2019 01/10/2018 31/12/2019 PORZIONATO ANDREA (Presidente)
DE CARO RAFFAELE (Membro Effettivo)
MACCHI VERONICA (Membro Effettivo)
2 COMMISSIONE D'ESAME DI ATTVITIA' DIDATTICA ELETTIVA DI ANATOMIA CLINICA A.A. 2016/2017 01/10/2016 31/12/2018 PORZIONATO ANDREA (Presidente)
DE CARO RAFFAELE (Membro Effettivo)
MACCHI VERONICA (Membro Effettivo)
1 COMMISSIONE D'ESAME DI ATTVITIA' DIDATTICA ELETTIVA DI ANATOMIA CLINICA A.A. 2015/2016 01/10/2015 31/12/2018 PORZIONATO ANDREA (Presidente)
DE CARO RAFFAELE (Membro Effettivo)
MACCHI VERONICA (Membro Effettivo)

Syllabus
Prerequisites: Knowledge of biology, molecular biology, anatomy, physiology and pathology.
Target skills and knowledge: - Methods of development of in vitro experimental models (primary cell cultures, established cell lines, stem cells).
- Knowledge of the biology, anatomy and physiology of the main animal species involved in in vivo experimental models (C. elegans, D. melanogaster, D. rerio, M. musculus, R. norvegicus, ...)
- Experimental models of diseases of the nervous, cardiovascular, respiratory, muscular, and metabolic systems.
Examination methods: Written examination
Course unit contents: - History of cell cultures. Types of cell cultures: primary cell cultures; established cell lines; stem cells. Methods to isolate primary cells: explant culture; dissociated cell cultures. Mechanical and enzymatic methods of disaggregation. Some examples of primary cell cultures: human umbilical vein endothelial cells (HUVEC), human umbilical artery smooth muscle cells (HUASMC), neuronal cell cultures, etc.. Hayflick limit. Some examples of established cell lines (HEK293, HeLa, CHO, COS7, HL60, Jurkat, …). Types of embryonic and adult stem cells. Somatic cell nuclear transfer. Single cell embryo biopsy. Arrested embryos. Altered nuclear transfer. Reprogramming. Amniotic fluid-derived stem cells.
- Features of a cell culture lab; optimal condition for cell culture: laminar flow cabinet, cell scaffold, growth media, balanced saline solutions, serum, growth media selection, other supplements, sterilization methods, cell adhesion. Preparation, propagation and conservation of suspension and adherent cell cultures: initial phases, trypsinization of monolayers, cell line evolution, clonal isolation, cryopreservation, senescence;
- Aseptic technique. Sterility assurance reduction. Cell culture contaminations: toxic chemicals, other cell lines, fungi, yeasts, viruses, bacteria (including mycoplasms). Antibiotics and antifungal drugs. Most commonly used citotoxicity parameters. Synchronized cell cultures: chemical and physical cell synchronization methods. Testing synchrony by autoradiography.
- Exogenous DNA introduction in mammalian cells: transfection methods, transient transfections and isolation of stable transfected cell lines. GFP and its applications in cell cultures;
- Animal model organisms: invertebrates (Caenorhabditis elegans, Drosophila melanogaster), fishes (Danio rerio), amphibians (Xenopus laevis), rodents (Rattus norvegicus, Mus musculus, Mesocricetus auratus, Meriones unguiculatus, Cavia porcellus), lagomorphs (Oryctolagus cuniculus), dogs, cats, and primates.
- The mouse as a model organism. Benefits of mouse for functional genomic studies in mammalians. General features of mouse (genome, life cycle, embryonic development, strains, etc.). Anatomy of the mouse and rat. Transgenesis in mouse: gene transfer technologies and main fields of application of transgenesis in medicine. Use and applications of transgenesis through DNA microinjection in the ovocyte.
- Zebrafish. Embryonic development. Comparative biology for modeling human diseases. Forward- and reverse-genetics approaches. Examples of the main zebrafish disease models.
- Drosophila melanogaster. General features and morphology. Life cycles. Disease models of neurodegenerative pathologies.
- C. elegans. General biology. Organization of the nervous system. Models of addiction and neurodegenerative diseases.
- Morphogenesis of the central nervous system. Adult neurogenesis in the subventricular zone and subgranular zone of the dentate gyrus. Neural stem cells and their use in in vivo experimental models of neurological diseases.
- Presentations about experimental models of nervous system diseases (Alzheimer, Parkinson, depression, ALS, Huntington’s disease, fragile X syndrome, spina bifida), cardiovascular diseases (myocardial infarction, atherosclerosis, hypertension), respiratory diseases (asthma), muscular diseases (Duchenne muscular dystrophy, Friedreich’s ataxia), hematopoietic disorders (leukemia), metabolic diseases (diabetes, metabolic syndrome), infective diseases (HIV, HHV6). Molecular mechanisms regulating drug addiction.
Planned learning activities and teaching methods: Frontal lessons and practical activities
Textbooks (and optional supplementary readings)