First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Medicine
Course unit
MEP3052619, A.A. 2019/20

Information concerning the students who enrolled in A.Y. 2015/16

Information on the course unit
Degree course 5 years single cycle degree in
FA1733, Degree course structure A.Y. 2009/10, A.Y. 2019/20
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Number of ECTS credits allocated 6.0
Type of assessment Mark
Course unit English denomination ANALYTICAL METHODS IN BIOCHEMISTRY
Department of reference Department of Pharmaceutical and Pharmacological Sciences
E-Learning website
Mandatory attendance
Language of instruction English
Single Course unit The Course unit CANNOT be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Teacher in charge BARBARA SPOLAORE BIO/10

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Core courses BIO/10 Biochemistry 6.0

Course unit organization
Period First semester
Year 5th Year
Teaching method frontal

Type of hours Credits Teaching
Hours of
Individual study
Laboratory 1.0 15 10.0 No turn
Lecture 5.0 40 85.0 No turn

Start of activities 30/09/2019
End of activities 18/01/2020
Show course schedule 2019/20 Reg.2009 course timetable

Examination board
Board From To Members of the board
7 Commissione a.a. 2019/20 01/12/2019 30/11/2020 SPOLAORE BARBARA (Presidente)
6 Commissione a.a. 2018/19 01/12/2018 30/11/2019 SPOLAORE BARBARA (Presidente)

Prerequisites: Students are expected to have a background in Biochemistry and Applied Biochemistry in order to better understand the analytical techniques decribed during the course and their applications.
Target skills and knowledge: At the end of the course, the student will known techniques used for the analysis of biomolecules and their use for diagnostic and pharmaceutical applications. The course also introduces the students to the interpretation of the results of these analyses, in particular of those obtained by mass spectrometry.
Examination methods: Assessment at the end of the course will be in English and it will be based on a written examination on the topics of the lessons. The written test lasts two hours and includes 1 exercise, a multiple choice test with 6 questions and 5 open questions.
The evaluation of the report of laboratory activity also contributes to the final mark. During the course, the teacher will provide a scheme of the report that has to be written in English.
Assessment criteria: Assessment at the end of the course aims to test the student's ability to critically describe the analytical techniques studied during the course and their applications. The final mark is determined for about 60% by the answers to the open questions and for 40% by the exercise, by the answers to the quiz and by the evaluation of the laboratory report.
Course unit contents: The course of Analytical Biochemistry deals with analytical techniques used for the analysis of biomolecoles, in particular proteins. The course will illustrate the developments of mass spectrometry and its applications in biochemical analyses with practical examples of interpretation of mass spectra. The course will also describe immunological techniques and radioisotope techniques. The final part of the course will show applications of these techniques in the clinics and in the pharmaceutical industry. The laboratory part will concern the use of immunological assays and the identification of proteins by mass spectrometry.
Planned learning activities and teaching methods: The course consists in class lectures and in one credit of laboratory. Some hours will be dedicated also to the interpretation of results obtained with the techniques described during the course.
Additional notes about suggested reading: Slides shown during the lectures are made available in Moodle. Articles and reviews are provided to students for home study. Students can find the topics of the course also in the reference books.
Textbooks (and optional supplementary readings)
  • Edmond de Hoffmann e Vincent Stroobant, Mass Spectrometry Principles and Applications. --: John Wiley & Sons Ltd., 2007. Testo di consultazione Cerca nel catalogo
  • Keith Wilson e John Walker, Principles and Techniques of Biochemistry and Molecular Biology. --: Cambridge University Press, 2010. Testo di consultazione Cerca nel catalogo
  • Susan R. Mikkelsen e Eduardo Cortòn, Bioanalytical Chemistry. --: John Wiley & Sons, Inc., 2004. Testo di consultazione Cerca nel catalogo
  • Jürgen H. Gross, Mass Spectrometry A Textbook. --: Springer-Verlag Berlin Heidelberg, 2011. Testo di consultazione Cerca nel catalogo

Innovative teaching methods: Teaching and learning strategies
  • Laboratory
  • Problem based learning
  • Case study
  • Interactive lecturing
  • Working in group
  • Use of online videos
  • Loading of files and pages (web pages, Moodle, ...)

Innovative teaching methods: Software or applications used
  • Moodle (files, quizzes, workshops, ...)