First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Medicine
CHEMISTRY AND PHARMACEUTICAL TECHNIQUES
Course unit
ANALYTICAL METHODS IN BIOCHEMISTRY
MEP3052619, A.A. 2018/19

Information concerning the students who enrolled in A.Y. 2014/15

Information on the course unit
Degree course 5 years single cycle degree in
CHEMISTRY AND PHARMACEUTICAL TECHNIQUES
FA1733, Degree course structure A.Y. 2009/10, A.Y. 2018/19
N0
bring this page
with you
Number of ECTS credits allocated 6.0
Type of assessment Mark
Course unit English denomination ANALYTICAL METHODS IN BIOCHEMISTRY
Department of reference Department of Pharmaceutical and Pharmacological Sciences
E-Learning website https://elearning.unipd.it/dsf/course/view.php?idnumber=2018-FA1733-000ZZ-2014-MEP3052619-N0
Mandatory attendance
Language of instruction English
Branch PADOVA
Single Course unit The Course unit CANNOT be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge BARBARA SPOLAORE BIO/10

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Core courses BIO/10 Biochemistry 6.0

Course unit organization
Period First semester
Year 5th Year
Teaching method frontal

Type of hours Credits Teaching
hours
Hours of
Individual study
Shifts
Laboratory 1.0 15 10.0 No turn
Lecture 5.0 40 85.0 No turn

Calendar
Start of activities 01/10/2018
End of activities 18/01/2019

Examination board
Board From To Members of the board
6 Commissione a.a. 2018/19 01/12/2018 30/09/2019 SPOLAORE BARBARA (Presidente)
DE FILIPPIS VINCENZO (Membro Effettivo)
POLVERINO DE LAURETO PATRIZIA (Supplente)

Syllabus
Prerequisites: Students are expected to have a background in Biochemistry and Applied Biochemistry in order to better understand the analytical techniques decribed during the course and their applications.
Target skills and knowledge: At the end of the course, the student will known techniques used for the analysis of biomolecules and their use for diagnostic and pharmaceutical applications. The course also introduces the students to the interpretation of the results of these analyses, in particular of those obtained by mass spectrometry.
Examination methods: Assessment at the end of the course will be based on a written examination in English on the topics of the lessons. The written test lasts two hours and includes 1 exercise, a multiple choice test with 6 questions and 5 open questions.
The evaluation of the laboratory activity report also contributes to the final mark. The teacher will provide during the course a scheme of the report that has to be written in English.
Assessment criteria: Assessment at the end of the course aims to test the student's ability to critically describe the analytical techniques studied during the course and their applications. The final mark is determined for about 60% by the answers to the open questions and for 40% by the exercise, by the answers to the quiz and by the evaluation of the laboratory report.
Course unit contents: The course of Analytical Biochemistry deals with analytical techniques used for the analysis of biomolecoles, in particular proteins. The course will illustrate the developments of mass spectrometry and its applications in biochemical analyses with practical examples of interpretation of mass spectra. The course will also describe immunological techniques and radioisotope techniques. The final part of the course will show applications of these techniques in the clinics and in the pharmaceutical industry. The laboratory part will concern the use of immunological assays and the identification of proteins by mass spectrometry.
Planned learning activities and teaching methods: The course consists in class lectures and in one credit of laboratory. Some hours will be dedicated also to the interpretation of results obtained with the techniques described during the course.
Additional notes about suggested reading: Slides shown during the lectures are made available in Moodle. Articles and reviews will be provided to students for home study. Students will find some information also in the reference books of the course.
Textbooks (and optional supplementary readings)
  • Edmond de Hoffmann e Vincent Stroobant, Mass Spectrometry Principles and Applications. --: John Wiley & Sons Ltd., 2007. Testo di consultazione Cerca nel catalogo
  • Keith Wilson e John Walker, Principles and Techniques of Biochemistry and Molecular Biology. --: Cambridge University Press, 2010. Testo di consultazione Cerca nel catalogo
  • Susan R. Mikkelsen e Eduardo Cortòn, Bioanalytical Chemistry. --: John Wiley & Sons, Inc., 2004. Testo di consultazione Cerca nel catalogo
  • Jürgen H. Gross, Mass Spectrometry A Textbook. --: Springer-Verlag Berlin Heidelberg, 2011. Testo di consultazione Cerca nel catalogo

Innovative teaching methods: Teaching and learning strategies
  • Lecturing
  • Laboratory
  • Problem based learning
  • Case study
  • Working in group
  • Problem solving
  • Use of online videos
  • Loading of files and pages (web pages, Moodle, ...)

Innovative teaching methods: Software or applications used
  • Moodle (files, quizzes, workshops, ...)

Sustainable Development Goals (SDGs)
Quality Education