First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Medicine
PHARMACY
Course unit
CLINICAL BIOCHEMISTRY
MEP3051904, A.A. 2018/19

Information concerning the students who enrolled in A.Y. 2014/15

Information on the course unit
Degree course 5 years single cycle degree in
PHARMACY (Ord. 2009)
FA1732, Degree course structure A.Y. 2009/10, A.Y. 2018/19
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Number of ECTS credits allocated 6.0
Type of assessment Mark
Course unit English denomination CLINICAL BIOCHEMISTRY
Department of reference Department of Pharmaceutical and Pharmacological Sciences
Mandatory attendance
Language of instruction Italian
Branch PADOVA
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge MARCELLA CANTON BIO/10

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Educational activities in elective or integrative disciplines BIO/12 Clinical Biochemistry and Biology 6.0

Course unit organization
Period First semester
Year 5th Year
Teaching method frontal

Type of hours Credits Teaching
hours
Hours of
Individual study
Shifts
Lecture 6.0 48 102.0 No turn

Calendar
Start of activities 01/10/2018
End of activities 18/01/2019
Show course schedule 2019/20 Reg.2018 course timetable

Examination board
Board From To Members of the board
6 Commissione a.a. 2018/19 03/12/2018 30/09/2019 CANTON MARCELLA (Presidente)
POLVERINO DE LAURETO PATRIZIA (Membro Effettivo)
LOPREIATO RAFFAELE (Supplente)

Syllabus
Prerequisites: Biochemistry, Molecular Biology, physiology, general pathology
Target skills and knowledge: Main methodologies of body fluid analysis used in clinical practice and partly available also in pharmacy. Technical, instrumental and biochemical concepts useful to understand the significance and limitations of the analytical methods. Ability of interpreting differential diagnosis of common diseases
Examination methods: Written examination with open questions
Assessment criteria: About each answer it will be evaluated:
- completeness of information
- ability to connect different concepts and subjects
Course unit contents: Biological samples - Bias sources
Analytical variability: precision, accuracy, sensitivity, prediction
Blood - Analysis and evaluation of circulating components
Spectroscopic techniques - UV-VIS - Determination of total plasma protein concentration
Fluorimetry and applications in clinical chemistry - Amino acido diseases
Chemiluminescence, Bioluminescence and applications in clinical chemistry
Enzymes: enzyme assays - optical test
Isoenzymes: clinical significance (LDH) - Electrophoresis (principles)
SDS-PAGE - Western blot - Isoelectric focusing: principles and applications - Bidimensional electrophoresis
Nucleic acid electrophoresis - Capillary electrophoresis – Chromatographic techniques - HPLC and applications
Kreatinin kinase - Gamma glutamyl transferase - Acid and alkaline phosphatases - Pancreatic amylase and lipase
Plasma proteins (albumins and globulins) – Pathological changes in plasma protein profile
Plasma lipids – Plasma lipid transport and metabolism: lipoproieins
Role of chylomicrons, VLDL, LDL in cholesterol and triglyceride transport and metabolism
Dyslipidemia and risk factors – Cholesterol and triglyceride assays: colorimetric and enzymatic methods
Antigens and aptens – Antibodies: structure and functions – Immunological methods and applications
Direct and indirect Coombs test – Titrimetric Methods involving a precipitation: single and double immunodiffusion, immunoelectrophoresis
Competitive labeled immunoassay (RIA) - ELISA e EMIT
Non-competitive immunoassay – Monoclonal and polyclonal antibodies – Mab in therapy.
Molecular biology techniques. Recombinant DNA, seq DNA, hybridization (gene probes) – PCR – Clinical applications
Flow cytometry: principles and applications
Basic concepts on carbohydrate metabolism – Glycemia: significance and hormonal regulation
Insulin: synthesis, secretion, metabolism, receptors – Diabetes: definition and classifications – Blood glucose measurement and evaluation
- Blood glucose determination methods – Autoantibodies – Glycated hemoglobins – Ketonemia and ketonuria – Albuminuria
- Hepatic function evaluation – Bilirubine test – Heme catabolism – Conjugated and unconjugated bilirubin
Jaundice – Significance of hepatic enzyme level increase
Albumin and hepatic diseases. Liver and coagulation. Antitripsin
Liver and protein catabolism. Blood ammonia and urea levels
Kidney: structure and functions – Clearance – Kreatinin: synthesis, significance, analysis methods – Urea: synthesis, significance, analysis methods – Uric acid: synthesis, significance, analysis methods
Urine assay: biochemistry and microscopy – Acid-base balance – Role of liver and kidney
- Hydroelectrolitic balance and Na+ homeostasys - Ca2+ homeostasys: PTH, Vit. D, Calcitonin – Hyper and hypocalcemia
- Thyroid: hormone synthesis, release, transport and related effects – Methods for thyroid function evaluation – Hypo and hyperthyroidism
- Cardiovascular disease markers
- Inflammation markers
- Cancer biomarkers
- Immunology: autoantibodies, immunodeficiency, monoclonal gammopathy
- Therapeutic drug monitoring
Planned learning activities and teaching methods: Frontal lecturing
Additional notes about suggested reading: Slides will be made available for students
Textbooks (and optional supplementary readings)
  • Federici, Giorgio, Medicina di laboratorio[a cura di] Giorgio Federici. Milano: McGraw-Hill education, 2014. Cerca nel catalogo
  • Bishop, Michael L., Clinical Chemistry: Principles, Techniques and Correlations. --: --, --. da consultare Cerca nel catalogo
  • Bishop, Michael L.; Fody, Edward P.; Schoeff, Larry E., Clinical chemistryprinciples, techniques, and correlationsMichael L. Bishop, Edward P. Fody, Larry E. Schoeff. Philadelphia: Wolters Kluwer, 2018. Cerca nel catalogo
  • Walker, John; Wilson, Keith, Principles and techniques of biochemistry and molecular biologyedited by Keith Wilson and John Walker. Cambridge: Cambridge University Press, 2010, --. Cerca nel catalogo

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