First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Science
INDUSTRIAL BIOTECHNOLOGY
Course unit
LARGE-SCALE CELL CULTURES AND BIOMOLECULES PRODUCTION
SCN1037574, A.A. 2017/18

Information concerning the students who enrolled in A.Y. 2017/18

Information on the course unit
Degree course Second cycle degree in
INDUSTRIAL BIOTECHNOLOGY
SC1731, Degree course structure A.Y. 2014/15, A.Y. 2017/18
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Number of ECTS credits allocated 8.0
Type of assessment Mark
Course unit English denomination LARGE-SCALE CELL CULTURES AND BIOMOLECULES PRODUCTION
Website of the academic structure http://biotecnologie.scienze.unipd.it/2017/laurea_magistrale
Department of reference Department of Biology
E-Learning website https://elearning.unipd.it/biologia/course/view.php?idnumber=2017-SC1731-000ZZ-2017-SCN1037574-N0
Mandatory attendance
Language of instruction English
Branch PADOVA
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge CHIARA RAMPAZZO BIO/06
Other lecturers VALENTINA GANDIN CHIM/08

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Educational activities in elective or integrative disciplines CHIM/08 Pharmaceutical Chemistry 3.0
Core courses BIO/06 Comparative Anatomy and Citology 5.0

Mode of delivery (when and how)
Period Second semester
Year 1st Year
Teaching method frontal

Organisation of didactics
Type of hours Credits Hours of
teaching
Hours of
Individual study
Shifts
Lecture 8.0 64 136.0 No turn

Calendar
Start of activities 26/02/2018
End of activities 01/06/2018

Examination board
Board From To Members of the board
6 PRODUZIONI INDUSTRIALI DI CELLULE E BIOMOLECOLE 2017/2018 01/10/2017 25/11/2018 RAMPAZZO CHIARA (Presidente)
GANDIN VALENTINA (Membro Effettivo)
MOGNATO MADDALENA (Supplente)
5 PRODUZIONI INDUSTRIALI DI CELLULE E BIOMOLECOLE 2016-2017 01/10/2016 30/11/2017 RAMPAZZO CHIARA (Presidente)
GANDIN VALENTINA (Membro Effettivo)
PONTARIN GIOVANNA (Supplente)

Syllabus
Prerequisites: Students are expected to have knowledge and competence of cellular and molecular biology and of biochemistry to be able to understand the fundamental aspects of mammalian large scale cell culture in upstream and downstream processes.
Target skills and knowledge: The course is designed to give students the necessary theoretical tools and information to be able to: - isolate a clonal mammalian cell line for the overproduction of a specific biomolecule, - choose the right bioreactor based on the properties of the biomolecule to be produced, - how to improve cellular vitality in a bioreactor, - how to improve the process productivity by optimizing medium composition and cellular metabolism, - which are the current biomolecules of pharmaceutical interest and how can they be produced, - how to expand in large scale embryonal or adult stem cell cultures for cell therapy applications.
Examination methods: Final exam will be oral. Students will be evaluated collegially by both professors.
Assessment criteria: The purpose of the oral examination is to assess the student knowledge on all the topics illustrated in the programme of the lecture course.
Course unit contents: Overview of the biopharmaceutical industry. Upstream and downstream processes. GMP/GLP regulatory requirements for processing biopharmaceuticals. Lab/pilot scale process to implement full manufacturing scale. Consistency and robustness in a fermentation process. Large scale mammalian cell culture. Cell line engineering techniques and common host cell lines used. Bioreactor operation mode: batch, fed batch, continuous and perfusion culture. Selection of bioreactor type (spinner flask, stirred tank). Attachment systems for cell cultivation in adhesion (plates, roller bottle, and stacked plate system) packed bed bioreactor, microcarriers, fluidized bed bioreactor, hollow fiber and wave bioreactor. Perfusion systems for cell cultivation (hollow fiber, spin filter, acoustic cell separation, alternating tangential flow (ATF) system). Design of cell culture medium without serum and with low content of proteins. Scaffold and matrix in bioreactors. How to calibrate oxygen, pH, nutrients and metabolites, cell density and viability in the bioreactor. Design of large scale cell culture process for mammalian cell culture. How to improve cell viability in a process. Expression of cloned proteins in mammalian cells, e.g. interferon and insulin. Large scale production of monoclonal antibodies and their use. Vaccine process development in mammalian cells and manufacturing of vaccins. Embryonic and adult stem cell cultures and their application in cell therapy. Biomolecules of pharmaceutical interest. Cytokines: interleukins and interferons. Hormons: insulin and growth hormone. Enzymes: tissue plasminogen activator and DNase. Erithropoietin. Heparin. Monoclonal antibodies: pharmaceutical and therapeutic properties. Monoclonal antibody-based drugs for antitumor, immunosuppressive, antithrombotic, antiviral, antiasthma and antiangiogenic therapies. Antisense oligonucleotides: approved agent and clinical trials. Aptamers. Ribozymes.
Planned learning activities and teaching methods: Frontal lectures and seminars by non accademic specialists of Industrial technologies for production and application of cells and biomolecules
Additional notes about suggested reading: As supplementary materials, power point slides of all lectures will available on https://elearning.unipd.it/biologia/,. Recent scientific publication on international journal useful for the understanding of specific topics will be suggested. All materials will be available once the student has registered on the web site.
Textbooks (and optional supplementary readings)
  • Butler M, CELL CULTURE AND UPSTREAM PROCESSING. --: TAYLOR AND FRANCIS, 2007. Cerca nel catalogo
  • G.Walsh, BIOPHARMACEUTICALS-BIOCHEMISTRY AND BIOTECHNOLOGY. --: WILEY, --. Cerca nel catalogo
  • Ozturk S.S. AND Hu W.S., CELL CULTURE TECHNOLOGY FOR PHARMACEUTICAL AND CELL-BASED THERAPIES. --: Taylor and Francis, 2006. Cerca nel catalogo
  • D.J.A. Crommelin, R.D. Sindelar, B. Meibohm,, PHARMACEUTICAL BIOTECHNOLOGY FUNDAMENTAL AND APPLICATIONS. --: INFORMA HEALTHCARE, --. Cerca nel catalogo