First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Science
SANITARY BIOLOGY
Course unit
APPLIED BIOCHEMISTRY
SCN1032659, A.A. 2017/18

Information concerning the students who enrolled in A.Y. 2017/18

Information on the course unit
Degree course Second cycle degree in
SANITARY BIOLOGY
SC1177, Degree course structure A.Y. 2008/09, A.Y. 2017/18
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Number of ECTS credits allocated 6.0
Type of assessment Mark
Course unit English denomination APPLIED BIOCHEMISTRY
Website of the academic structure http://biologiamolecolare.scienze.unipd.it/2017/laurea_magistrale_biologiasanitaria
Department of reference Department of Biology
Mandatory attendance
Language of instruction Italian
Branch PADOVA
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge PAOLA COSTANTINI BIO/10

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Core courses BIO/10 Biochemistry 6.0

Mode of delivery (when and how)
Period Second semester
Year 1st Year
Teaching method frontal

Organisation of didactics
Type of hours Credits Hours of
teaching
Hours of
Individual study
Shifts
Laboratory 3.0 48 27.0 No turn
Lecture 3.0 24 51.0 No turn

Calendar
Start of activities 26/02/2018
End of activities 01/06/2018

Syllabus
Prerequisites: A strong knowledge of structural and metabolic biochemistry is an essential requisite to fully acquire the contents of both lessons and laboratory sessions.
Target skills and knowledge: The main goal of the course is focused on the applications of the biochemical principles in medicine, particularly related to the metabolism and to the enzymatic alterations causing its dysregulation/dysfunction in selected examples. Several diagnostic enzymatic tests widely used in clinical biochemistry are presented and discussed by means of both theoretical lessons and practical laboratory sessions
Examination methods: Written test with five open questions
Assessment criteria: Evaluation criteria will be 1) the degree of knowledge, learning and understanding of the discussed topics, 2) the capability to apply the biochemistry theoretical principles to practical clinical examples, and 3) the aptitude to present and discuss selected scientific papers by means of journal club.
Course unit contents: Lessons (24 hours)
Clinical biochemistry
- The goals. Diagnosis and prognosis of diseases. The main metabolic processes of biochemical-clinical interest.
- The main hereditary metabolic deseases, particularly related to diagnosis and therapies.
- Clinical enzymology (8 hours)
i) The enzymes as analytical reagents (the immunoenzymatic assays in clinics). ii) The enzymes as diagnosis markers (i.e.: use of asparaginase for the treatment of ALL, acute lymphoblastic leukemia).
- Production of proteins of medical interest (diagnosis, therapies, vaccines) by means of genetic engeneering.
Methods for the formulation of laboratory data (4 hours)
Causes of analytical variability in the diagnostic tests (pre-analytical, analytical and post-analytical factors). Operative features of the diagnostic tests.
The interpretation of the diagnostic test analytical data (sensitivity and specificity, the "problem" of false positive/negative, ROC curves, predictive value of a diagnostic test).
Theoretical introduction to the laboratory sessions (2 hours)
Laboratory sessions (36 hours)
- Quantification of urea nitrogen and lactic acid in the serum by means of coupled enzymatic assays (4 hours)
- Quantification of glucose and creatine-kinase in the serum by means of coupled enzymatic assays (4 hours)
- Analysis of lactate-dehydrogenase activity by activity stain on agarose gel (8 hours)
- Electrophoretic analysis of lactate-dehydrogenase by SDS-PAGE and western blot (8 hours)
- Bi-dimensional electrophoresis of a protein extract from E. coli (8 hours)
- Purification of a recombinant protein by NiNTA technology (8 hours)
Journal Clubs (10 hours)
Selection of a scientific article focused on topics of biochemical/clinical interest presented during the lessons, elaboration by small groups and oral presentation (10 hours)
Planned learning activities and teaching methods: The course combines theoretical lessons with laboratory and journal club sessions, with the main goal to promote the knowledge and the capability to learn, understand and apply this knowledge (by reading reviews, looking for bibliographic material, also on line, referring to text books), team works (laboratory activities), capability of evaluation (critical discussion of the obtained results) and ability to talk to other people (oral presentation of a paper).
Additional notes about suggested reading: No books are required. Slides, papers and files containing description/protocols of laboratory sessions are provided by the teacher.
Textbooks (and optional supplementary readings)