First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Science
BIOTECHNOLOGY
Course unit
BIOCHEMISTRY I
SCP4068080, A.A. 2017/18

Information concerning the students who enrolled in A.Y. 2016/17

Information on the course unit
Degree course First cycle degree in
BIOTECHNOLOGY
IF1839, Degree course structure A.Y. 2011/12, A.Y. 2017/18
N0
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Number of ECTS credits allocated 6.0
Type of assessment Mark
Course unit English denomination BIOCHEMISTRY I
Website of the academic structure http://biotecnologie.scienze.unipd.it/2017/laurea
Department of reference Department of Biology
Mandatory attendance
Language of instruction Italian
Branch PADOVA
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge MARISA BRINI BIO/10

Integrated course for this unit
Course unit code Course unit name Teacher in charge
SCP4068079 BIOCHEMISTRY MARISA BRINI

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Basic courses BIO/10 Biochemistry 6.0

Mode of delivery (when and how)
Period Annual
Year 2nd Year
Teaching method frontal

Organisation of didactics
Type of hours Credits Hours of
teaching
Hours of
Individual study
Shifts
Laboratory 1.0 16 9.0 2
Lecture 5.0 40 85.0 No turn

Calendar
Start of activities 02/10/2017
End of activities 15/06/2018

Syllabus

Common characteristics of the Integrated Course unit

Prerequisites: Basic concepts of Physics, General and Organic Chemistry.
Elements of Chemical Thermodynamics,Kinetics and Electrochemistry
Target skills and knowledge: Knowledge of the essential molecules to the chemistry of living organisms.
Comprehension of the fundamental mechanisms at the basis of cellular function.
Learning of some biochemistry techniques.
Examination methods: Written examination with both multiple choice and open questions
Assessment criteria: The overall knowledge about the topics of Biochemistry 1 and 2 will be evaluated, considering positive the examinations which show an adequate assimilation of the topics necessary to the later classes

Specific characteristics of the Module

Course unit contents: Proteins. Amino acids: structure, properties and classification. Structures and properties of the peptide bond. Polypeptides chains. Levels of structure in protein architecture. Primary, secondary , tertiary and quaternary structures. Structural domains and structure/function relationship. Denaturation. Protein folding.

Oxygen-transporting proteins. Myoglobin and hemoglobin. Oxygen binding. Cooperative binding. Allosteric effects and allosteric factors.

Enzymes. Catalysis and enzyme kinetic. Michaelis–Menten model. Significance of vmax, Km and Kcat and their calculation. Energetic profile of an enzymatic reaction. Catalytic mechanisms. Regulation of enzymatic activity and mechanisms of inhibition.

Carbohydrates. Monosaccharides, disaccharides and polysaccharides. Glycoside bond. Glycoproteins, glycolipids and proteoglycans.

Lipids and Biological membranes. Structure and properties of lipids (fatty acids, triglycerides, waxes). Membrane lipids (glycerophospholipids, sphingolipids, glycolipids and cholesterol). Lipophilic vitamins. Structure and properties of the cellular membranes. Fluidity and asymmetry of the membranes. Membrane proteins and their structure. Transport across membranes: mechanisms and classification.

Cell Signalling. Examples of molecular mechanisms of signal transduction.

Practicals: Spectrophotometry theory and application. Absorbance spectrum of Flavin mononucleotide (FMN) in oxidized and reduced form. Determination of protein concentration by biuret method. Lactate dehydrogenase activity: experimental determination of vmax and Km values.
Planned learning activities and teaching methods: Lectures (40 hours)
Practicals (16 hours)
Additional notes about suggested reading: Slides are available on moodle (https://elearning.unipd.it/biologia/)

The study on the textbooks is strongly recommended
Textbooks (and optional supplementary readings)
  • David L. Nelson,Michael M. Cox, I principi di Biochimica di Lehninger. --: Zanichelli, --. Cerca nel catalogo
  • R.H. Garret, C.M. Grisham, Biochimica. --: Piccin, --.