First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Science
Course unit
SCO2044105, A.A. 2017/18

Information concerning the students who enrolled in A.Y. 2016/17

Information on the course unit
Degree course Second cycle degree in
SC1731, Degree course structure A.Y. 2014/15, A.Y. 2017/18
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Number of ECTS credits allocated 8.0
Type of assessment Mark
Course unit English denomination IMMUNOLOGICAL BIOTECHNOLOGY
Website of the academic structure
Department of reference Department of Biology
Mandatory attendance
Language of instruction English
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Teacher in charge EMANUELE PAPINI MED/04
Other lecturers REGINA TAVANO MED/04

Course unit code Course unit name Teacher in charge Degree course code

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Educational activities in elective or integrative disciplines MED/04 General Pathology 4.0
Core courses MED/04 General Pathology 4.0

Mode of delivery (when and how)
Period First semester
Year 2nd Year
Teaching method frontal

Organisation of didactics
Type of hours Credits Hours of
Hours of
Individual study
Laboratory 3.0 48 27.0 No turn
Lecture 5.0 40 85.0 No turn

Start of activities 02/10/2017
End of activities 19/01/2018

Prerequisites: The student must have a good preparation in general Immunolgy
Target skills and knowledge: Frontal lectures: To understand vaccinology in its basic medical terms, to have a view on the microbiological, molecular biology and chemical approach to design vaccine nowaday. To understand adjuvancy: empirically and rational design. To understand the connections with nanomedicine of vaccine design.

Laboratory: To be able to handle primary human immune cells in sterility conditions and to evaluate their responses in vitro. To perform complex immunology experimental protocols, to analyse data and draw proper conclusions from them.
Examination methods: Oral examination plus evaluation of a laboratory activity written report
Assessment criteria: The evaluation of the student will be done considering his/her skill in the arguments object of the course. The student ability to understand the experimental procedure perfomed and to draw conclusion independently will be appreciated.
Course unit contents: - Classic Vaccinology
- Main problems in the development of a vaccine.
- production of recombinant vaccines
- Microbial, animal and vegetal models for vaccine production.
- Reverse vaccinology: genome based antigen individuation (in silico). Production, quality control
Main vaccines in the pediatric prevention in Italy
ADjuvants - Mucosal adjuvant- micro-nanosized new generation adjuvants.
- Use of dendritic cells in therapy: prespectives.

Practical part:

Evaluation in vitro of adjuvancy in human dendritic cells. Isolation of monocytes fromm blood, thier differentiation into Dendritic Cells (DCs). Stimulation of DCs with various adjuvants and analysis of cell activation by Elisa (TNFa) and flow cytometry (CD86, CD11), RT-PCR (tnfa gene transcription). Aultologous/heterologous T lymphocytes proliferation and characterisation of their immunological competence by FACS.
Planned learning activities and teaching methods: Frontal lectures and laboratory activity (on dendritic cells)
Additional notes about suggested reading: A basic immunology test plus the material and slides provided by the teacher.
The protocols as provided.
lecture notes.
Textbooks (and optional supplementary readings)