First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Science
MOLECULAR BIOLOGY
Course unit
MOLECULAR AND CELL BIOLOGY OF PLANTS
SCN1028797, A.A. 2016/17

Information concerning the students who enrolled in A.Y. 2016/17

Information on the course unit
Degree course Second cycle degree in
MOLECULAR BIOLOGY
SC1175, Degree course structure A.Y. 2008/09, A.Y. 2016/17
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Degree course track Common track
Number of ECTS credits allocated 9.0
Type of assessment Mark
Course unit English denomination MOLECULAR AND CELL BIOLOGY OF PLANTS
Website of the academic structure http://biologiamolecolare.scienze.unipd.it/2016/laurea_magistrale_biologiamolecolare
Department of reference Department of Biology
E-Learning website https://elearning.unipd.it/biologia/course/view.php?idnumber=2016-SC1175-000ZZ-2016-SCN1028797-N0
Mandatory attendance
Language of instruction Italian
Branch PADOVA
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge BARBARA BALDAN BIO/01
Other lecturers LORELLA NAVAZIO BIO/01

Mutuated
Course unit code Course unit name Teacher in charge Degree course code
SC02119822 PLANT FUNCTIONAL MORPHOLOGY BARBARA BALDAN SC1179

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Core courses BIO/01 General Botanics 7.0
Core courses BIO/04 Vegetal Physiology 2.0

Mode of delivery (when and how)
Period First semester
Year 1st Year
Teaching method frontal

Organisation of didactics
Type of hours Credits Hours of
teaching
Hours of
Individual study
Shifts
Laboratory 1.0 16 9.0 No turn
Lecture 8.0 64 136.0 No turn

Calendar
Start of activities 01/10/2016
End of activities 20/01/2017

Examination board
Board From To Members of the board
6 BIOLOGIA MOLECOLARE E CELLULARE DELLE PIANTE 2017/2018 01/10/2017 25/11/2018 BALDAN BARBARA (Presidente)
NAVAZIO LORELLA (Membro Effettivo)
DALLA VECCHIA FRANCESCA (Supplente)
5 BIOLOGIA MOLECOLARE E CELLULARE DELLE PIANTE 2016-2017 01/10/2016 30/11/2017 BALDAN BARBARA (Presidente)
NAVAZIO LORELLA (Membro Effettivo)
DALLA VECCHIA FRANCESCA (Supplente)

Syllabus
Prerequisites: Students should have already acquired a basic knowledge of Cell Biology, Plant Biology, Biochemistry and Molecular Biology.
Target skills and knowledge: The aim of this course is, on the basis of the knowledge acquired during the first level degree, to study in depth some known subjects and to tackle new plant biology topics from a molecular and cellular point of view.
Examination methods: To verify the acquired knowledges the exam will be in written form, with open questions on theoretical arguments treated during the course; the active partecipation to the discussions proposed during teaching classrooms will also be considered.
Assessment criteria: Evaluation criteria will be:
- the degree of knowledge, learning and understanding of topics;
- the capability to answer to the proposed questions with critical aptitude and correct scientific language;
- the aptitude to present and discuss topics during the teaching classrooms.
Course unit contents: Growth and development: Shoot and root apical meristems: their establishment and maintenance. Determination of the developmental axes and the involved genes. Molecular aspects of lateral organ formation (6h).
Plant hormones (auxins, gibberellins, cytokinins, ethylene, abscissic acid): biosynthesis, actions, transport and developmental effects; signal transduction pathways (16h).
Blue light and red light responses: light perception, signal transduction and plant responses to light environmental conditions (5h).
Plant reproductive development: floral meristem development, floral organ identity genes, ABCDE model to explain the flower development; the control of flowering (5h).
Molecular aspects in micro and macro-gametogenesis; self-incompatibility during the pollen-pistil interactions; genes involved in control of double fertilization; embryo, seed and fruit development.
Protein secretion in plant cells: protein translocation and maturation in endoplasmic reticulum, ER quality control, anterograde and retrograde vesicular transport, protein sorting to protein-storing an lytic vacuoles; exocytosis ed endocytosis (8h).
Ca2+-mediated signal transduction in response to biotic and abiotic stresses in plants: Ca2+, an intracellular second messenger; methods of measuring intracellular Ca2+ concentration; calcium transients and calcium signatures (3h).
Programmed cell death in plants: cell death pathways in responses to biotic and abiotic inducers; morphological and biochemical markers; some examples of PCD during development and to cope environmental stresses (5h).
Plant–microorganism interactions: cellular and molecular surveys about mycorrhiza, Rhizobium-Leguminosae symbiosis and plant-Agrobacterium interaction (8h).

16h (1 CFU) of practical work are planned on the following topics:
1) RNA extraction from Arabidopsis thaliana and quantification
2) Analysis of gene expression by RT-PCR
3) Isolation of protoplasts from plant cell suspension cultures
4) Somatic embryogenesis in the model system Daucus carota, tobacco micropropagation
Planned learning activities and teaching methods: The course combines theoretical lessons with laboratory. The lab experience has some main goals: to understand and apply the acquired knowledge to practical activities (comprehension and application of experimental protocols) and to gain ability of evaluation (critical discussion of the obtained results).
Additional notes about suggested reading: Concerning some subjects, teachers will provide specific papers from specialized literature.
Selected slides, concerning topics not dealt with in the suggested textbook, will be provided on the e-learning platform.
Textbooks (and optional supplementary readings)
  • Taiz L, Zeiger E, Fisiologia Vegetale. Padova: Piccin Nuova Libraria, 2013. IV edizione italiana Cerca nel catalogo