First cycle
degree courses
Second cycle
degree courses
Single cycle
degree courses
School of Science
CHEMISTRY
Course unit
ANALYTICAL CHEMISTRY 2
SC09101131, A.A. 2017/18

Information concerning the students who enrolled in A.Y. 2015/16

Information on the course unit
Degree course First cycle degree in
CHEMISTRY
SC1156, Degree course structure A.Y. 2014/15, A.Y. 2017/18
N0
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Number of ECTS credits allocated 10.0
Type of assessment Mark
Course unit English denomination ANALYTICAL CHEMISTRY 2
Department of reference Department of Chemical Sciences
Mandatory attendance
Language of instruction Italian
Branch PADOVA
Single Course unit The Course unit can be attended under the option Single Course unit attendance
Optional Course unit The Course unit can be chosen as Optional Course unit

Lecturers
Teacher in charge PAOLO PASTORE CHIM/01
Other lecturers VALERIO DI MARCO CHIM/01
GABRIELLA FAVARO CHIM/01
MARCO FRASCONI CHIM/01

Mutuated
Course unit code Course unit name Teacher in charge Degree course code
SC09101131 ANALYTICAL CHEMISTRY 2 PAOLO PASTORE SC1157

ECTS: details
Type Scientific-Disciplinary Sector Credits allocated
Core courses CHIM/01 Analytical Chemistry 10.0

Mode of delivery (when and how)
Period First semester
Year 3rd Year
Teaching method frontal

Organisation of didactics
Type of hours Credits Hours of
teaching
Hours of
Individual study
Shifts
Laboratory 4.0 48 52.0 3
Lecture 6.0 48 102.0 No turn

Calendar
Start of activities 02/10/2017
End of activities 19/01/2018

Examination board
Board From To Members of the board
1 a.a. 2017/18 27/01/2014 30/11/2018 PASTORE PAOLO (Presidente)
BADOCCO DENIS (Membro Effettivo)
DI MARCO VALERIO (Membro Effettivo)
FAVARO GABRIELLA (Membro Effettivo)

Syllabus
Prerequisites: It is considered of extreme importance to have acquired significant knowlwdge in the field of General and inorganic Chemistry, organic chemistry and physical chemistry and have passed the examination of analytical chemistry I.
Target skills and knowledge: Aquisition of the basics of the most important instrumental analytical techniques and provide the basis for a proper use of instrumentation and analytical data.
The analytical chemistry II course offers the student the acquisition theoretical and manual skills related to the use of the main laboratory instrumental techniques by means of analytical determinations of both theoretical and practical interest in food and industrial environmental field. It also aims to provide a basic understanding of the main statistical methods for the treatment of analytical data.
Examination methods: The final examination consists of an oral test that will include an investigation on laboratory experiences.
Assessment criteria: The final score is expressed in thirtieths and is passed only with a score of at least 18/30.
Course unit contents: Methods of analysis used by the distribution between phases. Extraction. Distribution of one or more components between two phases. Sharing constant, distribution ratio, extracted fraction. Factors that govern the distribution and extraction. Gas chromatography. Theory of plates and kinetic, distribution profile, height equivalent to the theoretical plate (HETP). Influence of eluent flow rate and temperature on separation. Chromatographic resolution and efficiency. Qualitative and quantitative analysis. Instrumentation. Detectors, their efficiency, selectivity in the response, working conditions. Filled and and capillary columns. Chromatography on paper, TLC, HPLC. Chromatography in normal and reverse phase. Optimization of the eluent. Ion exchange and ion exchange chromatography with deletion. Characteristics of anion exchanger, selectivity. Size exclusion chromatography. Chromatography with supercritical fluids. Elettroanalysis. Electrochemical reactions: charge and mass transfer, flow equation. Voltammetrici methods. Polarographic methods, amperometry and bi-amperometry. Coulometry. Potentiometric, ISE methods. Conductometry. Spectrophotometric analysis methods. General information on electromagnetic radiation interaction on absorption. Spectrophotometric Analysis, UV-VIS, IR. Lambert-Beer's law and its deviations. Instrumentation. Spectrophotometric Titrations. Emission spectrometry, and atomic absorption. Atomizers and sources. Molecular fluorescence. Mass Spettromeria. Instrumentation. Production of ions, their separation and collection. Time-of-flight instrument quadrupole. Resolving power of a mass spectrometer. Gas-chromatograph coupled-MS: sample introduction systems. Ionisation methods and procedures. Electron ionization (EI), chemical ionization (CI), with laser (MALDI).

Statistical treatment of analytical data: a) basic definitions; b) distribution of the error and limit of detection; c) statistical tests; d) regression with the method of least squares; e) interference and effect matrix; f) quantitative analysis using calibration line (external calibration), the method of standard additions, use of internal standards. Laboratory experiences include the following techniques: gas chromatography, HPLC, ion chromatography, UV-VIS spectrophotometry, atomic absorption spectroscopy, potentiometry with ISE.
Planned learning activities and teaching methods: In the classroom, learning is facilitated by means of the view of the topics covered through the use of appropriate media. In laboratory, the learning is facilitated by the presence of numerous technical and professional figures alongside the teacher.
Additional notes about suggested reading: In addition to the recommended texts, handouts will be provided to the students, for the statistics part, as well as for laboratory experiences.
Textbooks (and optional supplementary readings)
  • Skoog, Douglas, Fondamenti di chimica analitica di Skoog e WestDouglas A. Skoog ... [et al.][edizione italiana a cura di Luigia Sabbatini]. Napoli: EdiSES, 2015. Cerca nel catalogo