IMMUNOLOGICAL BIOTECHNOLOGY

Second cycle degree in INDUSTRIAL BIOTECHNOLOGY

Campus: PADOVA

Language: English

Teaching period: First Semester

Lecturer: EMANUELE PAPINI

Number of ECTS credits allocated: 8


Syllabus
Prerequisites: The student must have a good preparation in general Immunolgy
Examination methods: Oral examination plus evaluation of a laboratory activity written report
Course unit contents: - Classic Vaccinology
- Main problems in the development of a vaccine.
- production of recombinant vaccines
- Microbial, animal and vegetal models for vaccine production.
- Reverse vaccinology: genome based antigen individuation (in silico). Production, quality control
Main vaccines in the pediatric prevention in Italy
ADjuvants - Mucosal adjuvant- micro-nanosized new generation adjuvants.
- Use of dendritic cells in therapy: prespectives.

Practical part:

Evaluation in vitro of adjuvancy in human dendritic cells. Isolation of monocytes fromm blood, thier differentiation into Dendritic Cells (DCs). Stimulation of DCs with various adjuvants and analysis of cell activation by Elisa (TNFa) and flow cytometry (CD86, CD11), RT-PCR (tnfa gene transcription). Aultologous/heterologous T lymphocytes proliferation and characterisation of their immunological competence by FACS.