NANOBIOTECHNOLOGY

Second cycle degree in MEDICAL BIOTECHNOLOGIES

Campus: PADOVA

Language: English

Teaching period: Second Semester

Lecturer: RAFFAELLA SIGNORINI

Number of ECTS credits allocated: 6


Syllabus
Prerequisites: No prerequisite
Examination methods: Two written exams with open question for each units, and an oral exam, when asked by the student, only for unit (i).
Course unit contents: Unit (i) Physical Chemistry of the Nanosystems:
Physical and chemical properties of bulk and nanomaterials: highlights on the electronic structure of atoms, molecules, metallic, semiconductors and insulating materials. Intermolecular forces and molecular recognition, surface interactions, molecular self-assembly. Structure and properties of metal, semiconductor and oxide nanoparticles.
Highlights on the techniques used to characterize nanomaterials:
X-ray diffraction, Optical and electronic microscopy, Scanning Probe Microscopy: STM and AFM, Absorption and emission spectroscopy, IR and Raman spectroscopy, Micro-raman and SERS.
Highlights on the use of nanoparticles and macromolecular compounds in the bio-medical field:


Unit (ii) Bio-Imaging

Electromagnetic waves
Refractive index
Propagation laws
Optical systems and image formation
Fluorescence: molecular apspects
Conventional fluorescence microscope
Point Spread Function (PSF) and resolving power of the microscope
Confocal Microscopy
Multiphoton Microscopy
STED Microscopy
Ca2+ Imaging
FRET
FRAP