LABORATORY MEDICINE TECHNOLOGIES AND MOLECULAR DIAGNOSTICS

Second cycle degree in MEDICAL BIOTECHNOLOGIES

Campus: PADOVA

Language: English

Teaching period: First Semester

Lecturer: ARIANNA LOREGIAN

Number of ECTS credits allocated: 14


Syllabus
Prerequisites: The course requires good knowledge of biochemistry, cellular biology, microbiology, genetics, and molecular biology.
Examination methods: The exam will consist in a written test.
Course unit contents: Molecular biology techniques based on nucleic acid amplification or hybridization. Techniques to study DNA methylation. Techniques for diagnosis of pathogenic microbes not based on molecular biology (isolation and identification of pathogenic bacteria, techniques for virological diagnosis not based on molecular biology). Therapeutic Drug Monitoring, TDM (pharmacokinetic parameters usually determined for TDM, methods for dosing drugs in biological samples, HPLC methods for dosing drugs, applications of TDM in diagnosis, practical lessons in a laboratory diagnostic unit performing TDM). Techniques to test the sensibility in vitro of microbial pathogens to drugs. Pharmacogenetics (basic principles and applications for diagnostic purposes, enzymes involved in drug metabolism, most common single nucleotide polymorphisms (SNPs) in the enzymes involved in drug metabolism and their pathogenetic relevance; microchips for diagnosis of these SNPs). Next-generation sequencing technologies, whole genome sequencing, metagenomics, paleogenomics, transcriptomics, metatranscriptomics, epigenomics, ChIP-sequencing, microbiome analysis. Validation and verification of diagnostic methods. Quality assurance in the clinical laboratory. Presentation and discussion of scientific articles describing recent and novel diagnostic techniques and their application.