PROTEIN ENGINEERING

Second cycle degree in PHARMACEUTICAL BIOTECHNOLOGIES

Campus: PADOVA

Language: English

Teaching period: Second Semester

Lecturer: DORIANNA SANDONA'

Number of ECTS credits allocated: 6


Syllabus
Prerequisites: Students must have a strong background in molecular biology, structural biochemistry and organic chemistry in order to understand the methods of production and modification of proteins and the rationale of the introduced mutations.
Examination methods: Assessment at the end of the course will be based on a written examination consisting of 6 questions (one question related on the practical part of the course) and an oral examination that will comprise also the critical exposition of a recent publication.
Course unit contents: The course deals with molecular biology and chemical methods that are essential to study the structure and function of proteins and to develop proteins with new properties.
The topics covered are as follows:
- chemical stability of proteins and strategies to improve it site
-methods of directed mutagenesis of proteins
-methods of random mutagenesis o proteins
-methodologies to create libraries of mutants (directed evolution) and methods of high-throughput screening
-methods for the production and modification of antibodies, antibody fragments and new binding proteins developed from non-immunoglobulin domains
-circular permutation of proteins
-inteins and their applications
-the chemical synthesis of peptides and proteins
-production of proteins and peptides containing non-natural amino acids.

Example of application in research and in biotechnology industry will be provided in order to better illustrate these techniques.