SOIL MICROBIOLOGY

Second cycle degree in SUSTAINABLE AGRICULTURE

Campus: LEGNARO (PD)

Language: English

Teaching period: Second Semester

Lecturer: ANDREA SQUARTINI

Number of ECTS credits allocated: 6


Syllabus
Prerequisites: Having already attended courses covering the topics of: general microbiology, agricultural microbiology, environmental microbiology, during the previous bachelor’s degree.
Examination methods: Oral examination after the end of the course
Course unit contents: • Soil microbial diversity as a function of environmantal variables
• DNA as the catalogue of all soil bacteria and fungi
• Automated DNA extraction from soil
• DNA-based strain fingerprinting in soil bacteria
• Soil typing by microbial DNA polymorphism for regional and local origin guarantee and sample traceability
• Soil species diversity analysis at strain level
• Bacteria distribution across the soil profile
• Nitrogen dynamics in cropped soils
• Probes for soil fertility assessment by in-farm measurements
• Soil respiration measurements with laboratory and field methodologies
• Effects of soil agricultural treatments on microbiota
• Analysis of bacterial diversity in rhizosphere and rhizoplane
• Plant-Microbe interactions – from soil to leaf
• Computer-assisted analysis of plant colonization by microorganisms
• Bacterial endophytes detection by digital PCR
• Microbial involvement in organic and biodynamic farming practices
• Comparison of conventional vs. organic farming by soil biotic indicators
• Risk assessment for genetically modified microorganisms release in agricultural fields
• Focus on techniques for soil microbiology
• Milestones/highly cited scientific papers